| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 972,121 | T→A | 9.3% | L137Q (CTG→CAG) | secD → | protein translocase subunit SecD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 972,121 | 0 | T | A | 9.3% | 124.8 / 4.5 | 43 | L137Q (CTG→CAG) | secD | protein translocase subunit SecD |
| Reads supporting (aligned to +/- strand): ref base T (18/21); new base A (2/2); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
GCCAGCCAAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGAC > NC_002947/971972‑972260 | gccagccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCctgct < 2:306888/150‑1 (MQ=255) gccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGaa > 2:470500/1‑150 (MQ=255) caAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGGTGGAAGt > 1:330575/1‑150 (MQ=255) caAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGt > 2:352465/1‑150 (MQ=255) aaGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtg < 2:407083/150‑1 (MQ=255) aaGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtg > 2:702761/1‑150 (MQ=255) aGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGCGGCCCTGAACCTGGCCCAGAGTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgg < 2:824043/150‑1 (MQ=255) ggATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTCCACTTCCAGCAGGAAGtgca < 2:506048/150‑3 (MQ=255) ggATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCACGATGAAACTGGGCCTGGACCTGTTCGGTGGTGTGCACTTCCTGCTGGAATtgga > 2:192779/1‑150 (MQ=255) gATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggac < 2:518804/150‑1 (MQ=255) aTGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtggaca < 1:229126/150‑1 (MQ=255) cAAGGCACTGGGTGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGc < 2:388791/150‑1 (MQ=255) tGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAg > 1:491372/1‑140 (MQ=255) tGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAg < 2:491372/140‑1 (MQ=255) gCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGccc > 2:686200/1‑150 (MQ=255) tACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTACTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGaaa < 1:504469/150‑1 (MQ=255) ccTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCAt > 1:508944/1‑123 (MQ=255) ccTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCAt < 2:508944/123‑1 (MQ=255) tGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTACGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTAAGGAGGCg > 1:273384/1‑150 (MQ=255) aaCCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTCGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAg < 1:742512/150‑1 (MQ=255) aaCCTGGCCCAGACGACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAg < 2:394953/150‑1 (MQ=255) gCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgga < 1:68950/98‑1 (MQ=255) gCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGtgga > 2:68950/1‑98 (MQ=255) cccAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAg < 1:146883/138‑1 (MQ=255) cccAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGCCTACGAAg > 2:146883/1‑138 (MQ=255) ccAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGAc < 1:686200/150‑1 (MQ=255) cAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGAcc > 1:412287/1‑150 (MQ=255) cGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTCCACTTCCAGCAGGAAGtgcac > 1:733753/1‑83 (MQ=38) cGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTCCACTTCCAGCAGGAAGtgcac < 2:733753/86‑1 (MQ=38) cAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCaaa < 2:447381/150‑1 (MQ=255) cAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTAAGAAGGCGAGGTCAAGACCTTGCTGCGCaaa < 1:470500/150‑1 (MQ=255) aaCTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAgg > 1:180641/1‑150 (MQ=255) aaCTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAgg > 1:682568/1‑150 (MQ=255) tGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCa > 1:81188/1‑132 (MQ=255) tGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCa < 2:81188/132‑1 (MQ=255) tGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATg > 2:208532/1‑150 (MQ=255) ggACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGc < 2:497164/60‑1 (MQ=255) ggACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGc > 1:497164/1‑60 (MQ=255) cGGTGGTGTGCACATCCGGATGGAAATGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTggg < 2:689639/150‑1 (MQ=255) tgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAgcctgc > 2:557989/1‑114 (MQ=255) tgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAgcctgc < 1:557989/114‑1 (MQ=255) gCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGa < 1:702761/150‑1 (MQ=255) gCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGa < 1:208532/150‑1 (MQ=255) cACTTCCAGCAGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGAc > 2:540013/1‑150 (MQ=255) tgGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTcag < 2:249207/107‑1 (MQ=255) tgGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTcag > 1:249207/1‑107 (MQ=255) | GCCAGCCAAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGAC > NC_002947/971972‑972260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |