Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 94,007 G→A 8.9% G102S (GGC→AGC)  trpI → HTH‑type transcriptional regulator TrpI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294794,0070GA 8.9% 118.6 / 3.0 45G102S (GGC→AGC) trpIHTH‑type transcriptional regulator TrpI
Reads supporting (aligned to +/- strand):  ref base G (24/17);  new base A (2/2);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

GTTCGTCAAGGACGGGCGTGGCATCAAACTCACAGATGCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGGTGTATGCCGAGCCACC  >  NC_002947/93859‑94155
                                                                                                                                                    |                                                                                                                                                    
gTTCGTCAAGGACGGGCGTGGCATCAAACTCACAGATGCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCggg                                                                                                                                                     >  2:404905/1‑150 (MQ=255)
        aGGACGGGCGTGGCATCAAACTCACAGATGCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCctgc                                                                                                                                             <  1:777701/150‑1 (MQ=255)
                       tCAAACTCACAGATGCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTa                                                                                                                              >  2:446275/1‑150 (MQ=255)
                       tCAAACTCACAGATGCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTa                                                                                                                              <  2:569048/150‑1 (MQ=255)
                          aagtggcaGATGCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCGGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTc                                                                                                                           >  2:384531/7‑150 (MQ=255)
                             tCACAGATGCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCcgc                                                                                                                        >  2:65933/1‑150 (MQ=255)
                                   aTGCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTgg                                                                                                                  >  2:91414/1‑150 (MQ=255)
                                     gCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTgggg                                                                                                                <  2:103665/150‑1 (MQ=255)
                                               gTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGTGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAgg                                                                                                      >  1:104555/1‑150 (MQ=255)
                                               gTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAgg                                                                                                      >  2:342307/1‑150 (MQ=255)
                                                tCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGc                                                                                                     >  1:283757/1‑150 (MQ=255)
                                                   gcgcGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGa                                                                                                  >  2:270728/1‑150 (MQ=255)
                                                     gcgATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCACTCGGGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATCCACCGCCTGGGACGGTTGCAGGCGGAtt                                                                                                >  1:232498/1‑150 (MQ=255)
                                                            cAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGtt                                                                                                                                >  1:168254/1‑111 (MQ=255)
                                                            cAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGtt                                                                                                                                <  2:168254/111‑1 (MQ=255)
                                                                    aGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTAtt                                                                                                                            >  1:636594/1‑107 (MQ=255)
                                                                    aGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTAtt                                                                                                                            <  2:636594/107‑1 (MQ=255)
                                                                         ttCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGNGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCAc                                                                            >  1:425962/1‑150 (MQ=255)
                                                                            gACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTg                                                                         >  2:326625/1‑150 (MQ=255)
                                                                             aCCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGt                                                                        <  1:326625/150‑1 (MQ=255)
                                                                             aCCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGt                                                                        >  2:559801/1‑150 (MQ=255)
                                                                                     cgcAGCGTGTGTTCCGAGCTCCGCCGTAATATAAGCGAGGCGCCATTCGTGCTTGGCTGCTCGGGCAGCCTGCTGTCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGt                                                                <  1:404905/150‑1 (MQ=255)
                                                                                     cgcAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGt                                                                <  1:65933/150‑1 (MQ=255)
                                                                                              tgtTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGAc                                                       <  2:478561/150‑1 (MQ=255)
                                                                                                ttCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCt                                                     >  1:847441/1‑150 (MQ=255)
                                                                                                ttCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCCCGAGCAGccca                                                                                                                                              >  1:454703/1‑59 (MQ=255)
                                                                                                ttCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCCCGAGCAGccca                                                                                                                                              <  2:454703/61‑3 (MQ=38)
                                                                                                            ccGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCgcggcg                                         >  2:490074/1‑150 (MQ=255)
                                                                                                             cGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGcggcgg                                        >  2:614107/1‑150 (MQ=255)
                                                                                                                   gtgAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCggg                                  >  2:798314/1‑150 (MQ=255)
                                                                                                                    tgAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGTTCTGCGGCGGCCCGGGc                                 <  2:847441/150‑1 (MQ=255)
                                                                                                                       gCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGTGTGCGTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTgg                              <  1:270728/150‑1 (MQ=255)
                                                                                                                         gAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGAt                            <  2:283757/150‑1 (MQ=255)
                                                                                                                           ggCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGc                          >  1:201244/1‑150 (MQ=255)
                                                                                                                            gcgcCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGACAGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCg                         >  1:433867/1‑150 (MQ=255)
                                                                                                                              gcCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGtt                       >  2:655367/1‑150 (MQ=255)
                                                                                                                                 aTTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCt                    >  1:117061/1‑150 (MQ=255)
                                                                                                                                   tCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTgg                  <  2:479042/150‑1 (MQ=255)
                                                                                                                                    cGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTTCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGgt                 <  2:117061/150‑1 (MQ=255)
                                                                                                                                    cGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGgt                 <  2:232498/150‑1 (MQ=255)
                                                                                                                                            gctgccCGAGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGGTGTAt             <  2:257442/146‑1 (MQ=255)
                                                                                                                                            gctgccCGAGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGGTGTAt             >  1:257442/1‑146 (MQ=255)
                                                                                                                                             ctgctCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGGTGTATGCCGa        <  1:446275/150‑1 (MQ=255)
                                                                                                                                              tgctCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGGTGTATGCCGAg       <  1:614107/150‑1 (MQ=255)
                                                                                                                                                 tCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGGTGTATGCCGAGCCa    >  2:714635/1‑150 (MQ=255)
                                                                                                                                                 tCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGGTGTATGCCGAGCCa    >  1:227698/1‑150 (MQ=255)
                                                                                                                                                   gggCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGGTGTATGCCGAGCCAcc  <  2:227698/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTCGTCAAGGACGGGCGTGGCATCAAACTCACAGATGCCGGTATGCGTCTGCGCGATGCCAGCGGTGAGGCCTTCGACCGGCTGCGCAGCGTGTGTTCCGAGCTCAGCCGTGATGTGAGCGAGGCGCCATTCGTGCTGGGCTGCTCGGGCAGCCTGCTGGCGCGGTGGTTTATTCCGCGGCTGGGGCGGTTGCAGGCGGATTTGCCTGAGCTGCGCTTGCACCTGTCGGCGGGTGAGGGCGACCTTGACCCGCGGCGGCCCGGGCTGGATGCGTTGCTGGTGTATGCCGAGCCACC  >  NC_002947/93859‑94155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑