Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 122,485 C→G 12.1% L158F (TTG→TTC)  PP_0116 ← putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947122,4850CG12.1% 82.8 / 3.0 31L158F (TTG→TTC) PP_0116putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (11/16);  new base G (2/2);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCGCTTTTCCAGCAAAGTCTTCAGCCAAGGCGCATCCTCGGTGCGCGGTACCTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCCGCCAGTGACCGCTGGCGCCTGCG  >  NC_002947/122345‑122633
                                                                                                                                            |                                                                                                                                                    
aCGCGCTTTTCCAGCAAAGTCTTCAGCCAAGGCGCATCCTCGGTGCGCGGTACCTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGCCTTGAAGAccact                                                                                                                                             <  2:748652/150‑2 (MQ=255)
          ccAGCAAAGTCTTCAGCCAAGGCGCATCCTCGGTGCGCGGTACCTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGCCTTGAAGAccact                                                                                                                                             >  1:335931/1‑139 (MQ=255)
          ccAGCAAAGTCTTCAGCCAAGGCGCATCCTCGGTGCGCGGTACCTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGCCTTGAAGAccact                                                                                                                                             <  2:335931/140‑2 (MQ=255)
                 aGTCTTCAGCCAAGGCGCATCCTCGGTGCGCGGTACCTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGt                                                                                                                            <  1:308771/150‑1 (MQ=255)
                  gTCTTCAGCCAAGGCGCATCCTCGGTGCGCGGTACCTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTc                                                                                                                           <  2:660651/150‑1 (MQ=255)
                         gCCAAGGCGCATCCTCGGTGCGCGGTAACTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCga                                                                                                                    <  1:827913/150‑1 (MQ=255)
                                 gcATCCTCGGTGCGCGGTACCTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGt                                                                                                            >  1:143654/1‑150 (MQ=255)
                                 gcATCCTCGGTGCGCGGTACCTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGt                                                                                                            <  2:360725/150‑1 (MQ=255)
                                            gcccgGTACCTGGTATGCCACCTCACACTGGTAACTCACCACATCGGAGGTTATTTCGTTCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGACGCTGTCGGGTCGATGATGCGTGCCTCGggctt                                                                                                 <  2:415474/147‑1 (MQ=255)
                                                          aTACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCtt                                                                                   >  1:227218/1‑150 (MQ=255)
                                                              caccTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGggctt                                                                                                 >  2:586411/1‑132 (MQ=255)
                                                              caccTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGggctt                                                                                                 <  1:586411/132‑1 (MQ=255)
                                                               cccTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTTGCTTCGGCGGCTTTCAgc                                                                              <  2:602202/149‑1 (MQ=255)
                                                                    acacTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGcgccgc                                                                         >  2:802317/1‑150 (MQ=255)
                                                                      acTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTc                                                                                                                                                      >  1:398285/1‑71 (MQ=255)
                                                                      acTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTc                                                                                                                                                      <  2:398285/71‑1 (MQ=255)
                                                                         ggTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCg                                                                                                             <  1:494915/109‑1 (MQ=255)
                                                                         ggTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCg                                                                                                             >  2:494915/1‑109 (MQ=255)
                                                                          gTAACTCACCACATCGGCGGCAATGTGGTCCGGTTGGCGGCGCGGCGCACGGCAGTCCGTGTTCTTGAAGGCCACCACGGGACTTGGCGATGTAGGGTGGATTATGCGTGCCTTGGGCTTGGGTTCGGGGGCTATCATCGCCACTTCAGc                                                                   >  2:729277/1‑150 (MQ=255)
                                                                           tAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGcc                                                                  <  2:227218/150‑1 (MQ=255)
                                                                                      aTCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAACCTTTTCCAGCTc                                                       <  2:243443/150‑1 (MQ=255)
                                                                                             gcAATGTAGTACTGTTGGCGGCGCAGCGCACGGCTGTAAATGGTCTTCAAGGACATCACGGTACTTGGCGCTGTCGGTTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTg                                                <  2:151659/150‑1 (MQ=255)
                                                                                                      tcCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTgg                                       <  1:802317/150‑1 (MQ=255)
                                                                                                                   gcagcgcaCGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGggcttg                                                                                                <  2:367664/80‑1 (MQ=255)
                                                                                                                   gcagcgcaCGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGggcttg                                                                                                >  1:367664/1‑80 (MQ=255)
                                                                                                                     agcgcaCGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCCg                        >  1:114579/1‑150 (MQ=255)
                                                                                                                        gcaCGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCCCGGCCTTGCGAGCCTGGGTCACGGCATCCCCGCCa                     <  1:245120/150‑1 (MQ=255)
                                                                                                                            ggCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCCGCCAGTGa                 >  2:54324/1‑150 (MQ=255)
                                                                                                                                   aGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCCGCCAGTGACCGCTgg          >  2:431410/1‑150 (MQ=255)
                                                                                                                                          ttCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCCGCCa                     <  1:696208/132‑1 (MQ=255)
                                                                                                                                          ttCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCCGCCa                     >  2:696208/1‑132 (MQ=255)
                                                                                                                                          ttCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCCGCCAGTGACCGCTGGCGCCTGc   >  2:531605/1‑150 (MQ=255)
                                                                                                                                          tccAAGCCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGGGGCTTTCAGCGCCGATTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCGGCTAGTGACCGCTGGCGCCTGc   <  2:130649/148‑1 (MQ=255)
                                                                                                                                           tCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCCGCCAGTGACCGCTGGCGCCTGCg  <  2:114579/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
ACGCGCTTTTCCAGCAAAGTCTTCAGCCAAGGCGCATCCTCGGTGCGCGGTACCTGGAATACCACCTCACACTGGTAACTCACCACATCGGCGGCAATGTCGTCCAGTTGGCGGCGCAGCGCACGGCTGTCAGTGGTCTTCAAGGCCACCACGGTACTTGGCGCTGTCGGGTCGATGATGCGTGCCTCGGGCTTGGCTTCGGCGGCTTTCAGCGCCGCTTCAGCCTTTTCCAGCTCGGCCTTGCGAGCCTGGGTCACGGCATCCCCGCCAGTGACCGCTGGCGCCTGCG  >  NC_002947/122345‑122633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑