| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,039,269 | A→T | 11.1% | A43A (GCT→GCA) | PP_0897 ← | class 1 fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,039,269 | 0 | A | T | 11.1% | 92.2 / 4.3 | 36 | A43A (GCT→GCA) | PP_0897 | class 1 fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (18/14); new base T (2/2); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01 | |||||||||||
GCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCT > NC_002947/1039128‑1039412 | gCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGGGTTGATCAGGTTCTGGGCGATGGAATCGCGTGCAG‑CAGGCGa < 1:339862/150‑1 (MQ=255) tCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGAtt > 2:346918/1‑150 (MQ=255) aTGGTGGCGCCATCCCAGCGCACGTCAATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGGAG‑CAGGCGATTcg > 2:429650/1‑149 (MQ=255) tggtggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCt < 1:840823/150‑1 (MQ=255) gtggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCCTGCTG‑CACGCGATTccatc < 2:680934/150‑4 (MQ=255) gcgcCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGGGCGCCCGTCACGATAGCGGCTAGTTAAGCTCGTGCAT‑CAGGCGATTCCTCACGc > 2:437857/1‑150 (MQ=255) gcCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGCCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAg > 1:460583/1‑150 (MQ=255) cccAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCCTGCTG‑CACGCGAtt > 2:592309/1‑135 (MQ=255) cccAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCCTGCTG‑CACGCGAtt < 1:592309/135‑1 (MQ=255) cAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGcc > 1:356424/1‑150 (MQ=255) cAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATCACCGGTGGCGCAAATGCGAGAGTGGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCGCGCGGAGAGGcc > 1:278011/1‑150 (MQ=255) aCGGCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTAGACGAGGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGccatgcac < 1:8913/150‑7 (MQ=255) ccATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATgg > 2:172668/1‑150 (MQ=255) aTGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGcc > 1:383749/1‑150 (MQ=255) acacGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACgg > 2:434599/1‑150 (MQ=255) aCCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑AAGGCGATTCCTCAGCCAGGTAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACga < 1:233691/150‑1 (MQ=255) tGGCAGATTGGACGATGGCCTGTGGCGCACATGCGCGAGTTGATCAGAATCGGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATGGc > 1:411009/1‑150 (MQ=255) tGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGaaa < 2:518133/120‑1 (MQ=255) tGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGaaa > 1:518133/1‑120 (MQ=255) tGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAAtt > 1:765545/1‑148 (MQ=255) tGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAAtt < 2:765545/148‑1 (MQ=255) gCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACgg > 2:631331/1‑125 (MQ=255) gCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACgg < 1:631331/125‑1 (MQ=255) gACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGc > 2:301880/1‑95 (MQ=255) gACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGc < 1:301880/95‑1 (MQ=255) caTGCGCGAGTTGATCAGGATCTGGGCGATGGAATCG‑GCGAATTCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGgtc < 1:437857/150‑1 (MQ=255) gcgAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTctt > 2:103619/1‑150 (MQ=255) aGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTcttgct < 2:356424/150‑1 (MQ=255) gTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTcttgctt > 2:693227/1‑150 (MQ=255) tGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGa < 1:346918/150‑1 (MQ=255) aTCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCa > 2:179404/1‑133 (MQ=255) aTCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCa < 1:179404/133‑1 (MQ=255) tGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAg > 1:240988/1‑125 (MQ=255) tGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAg < 2:240988/125‑1 (MQ=255) aTCGCCTGCTG‑CACGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGctcc > 1:75752/1‑150 (MQ=255) gcgTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCctct < 2:383749/150‑1 (MQ=255) cag‑cagGCGATTCCTCACGCAGATAGGACTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACg > 1:340508/1‑125 (MQ=255) cag‑cagGCGATTCCTCACGCAGATAGGACTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACg < 2:340508/125‑1 (MQ=255) | GCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAG‑CAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCT > NC_002947/1039128‑1039412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |