Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,049,192 C→A 11.4% L137L (CTG→CTT)  PP_0907 ← RND transporter membrane fusion protein‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,049,1920CA11.4% 91.1 / 2.6 36L137L (CTG→CTT) PP_0907RND transporter membrane fusion protein‑like protein
Reads supporting (aligned to +/- strand):  ref base C (14/17);  new base A (2/2);  total (16/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

TTTCTTGATCTTCTGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCAGATCCACGCCCTGCA  >  NC_002947/1049048‑1049340
                                                                                                                                                |                                                                                                                                                    
tttCTTGATCTTCTGCGCCTCAAGCGGCTCGACCCCCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTGGGCCTGGCCAAGGTc                                                                                                                                                 <  1:84115/150‑1 (MQ=255)
        tcttctGCTCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGTAAAGGCGGTCGAACTAGCCGCCGGAGATGGCCGAATCGCCCACCAGCCAGCTGCCTCGTCCGAGGTCGACCTTGGCTCGTCCCAGGTGGGCTAggg                                                                                                                                         >  2:159564/1‑150 (MQ=255)
        tcttctGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAggg                                                                                                                                         <  2:690529/150‑1 (MQ=255)
              gcgcCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTAc                                                                                                                                   >  2:600418/1‑150 (MQ=255)
                  cTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCAGCCAGGGCGGTACCGAg                                                                                                                               <  2:628611/150‑1 (MQ=255)
                    agccctgctCGACCACCGCCTGGTTGCGCAGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGGCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCa                                                                                                                             >  2:248856/5‑150 (MQ=255)
                     aaGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAg                                                                                                                            <  1:251910/150‑1 (MQ=255)
                         tgctCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCTAATTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCt                                                                                                                        <  1:248856/150‑1 (MQ=255)
                            tCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGtt                                                                                                                     <  1:73361/150‑1 (MQ=255)
                              gACCACCGCCTGGTTGCGCCGGTACTGGTAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACACGCTCGCTGCCACGGCCACAATCAGCACAAGGCTGTCCGATGTCGTCCAGGGCGGTACCGAGCAGGGCTGTTTc                                                                                                                   <  1:149556/150‑1 (MQ=255)
                                 caccGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCATCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAg                                                                                                                                           >  1:105250/1‑123 (MQ=255)
                                 caccGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAg                                                                                                                                           <  2:105250/123‑1 (MQ=255)
                                    cGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGAtc                                                                                                             >  2:671798/1‑150 (MQ=255)
                                                 cGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGc                                                                                                >  2:131380/1‑150 (MQ=255)
                                                   gTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGt                                                                                                                                                  >  1:566855/1‑98 (MQ=255)
                                                   gTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGt                                                                                                                                                  <  2:566855/98‑1 (MQ=255)
                                                          gaggaAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGt                                                                                       >  1:9787/1‑150 (MQ=255)
                                                           aggaAAGGCGGTCGAACTCGCCGCAGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGtt                                                                                      <  1:293/150‑1 (MQ=255)
                                                             gaAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCGCGAGCAGTTGc                                                                                    >  1:177174/1‑150 (MQ=255)
                                                                        gAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGAc                                                                         <  2:289065/150‑1 (MQ=255)
                                                                              gccgcGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCCAATGGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTg                                                                   <  1:199065/150‑1 (MQ=255)
                                                                                 gcgGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGcc                                                                <  2:209296/150‑1 (MQ=255)
                                                                                      agaTGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGt                                                           <  1:131380/150‑1 (MQ=255)
                                                                                          ggCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGaa                                                       <  1:403132/150‑1 (MQ=255)
                                                                                           gCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAg                                                      >  2:221048/1‑150 (MQ=255)
                                                                                            ccGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGt                                                     <  1:600418/150‑1 (MQ=255)
                                                                                                aTCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAg                                                 <  1:635586/150‑1 (MQ=255)
                                                                                                      caccaGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCATGAGTCGAAGTGCAGGGAttt                                           >  2:362947/1‑150 (MQ=255)
                                                                                                                         ccGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTcc                        >  2:144580/1‑150 (MQ=255)
                                                                                                                          cGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCa                       >  2:497029/1‑150 (MQ=255)
                                                                                                                            aaGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGAAGTTGCCCGGCATTGACCTGTTGGCCTGAGTAGAAGTGAAGAGATTTGACGATGCCTGCCACCTCCAgg                     <  2:9787/150‑1 (MQ=255)
                                                                                                                                cGACCTGGGCCTGGCCAAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCa                 >  2:486771/1‑150 (MQ=255)
                                                                                                                                 gACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGAt                                     >  1:811787/1‑129 (MQ=255)
                                                                                                                                 gACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGAt                                     <  2:811787/129‑1 (MQ=255)
                                                                                                                                          ctggCCAAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGc                                                                               <  2:594575/76‑1 (MQ=255)
                                                                                                                                          ctggCCAAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGc                                                                               >  1:594575/3‑78 (MQ=255)
                                                                                                                                               ccAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCAGATCCACGCCCTGCa  >  1:603549/1‑150 (MQ=255)
                                                                                                                                               acAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTGGACGATGCCTTCCACCTCCAGGCTCAGATCCACGCCCTGCa  >  2:585812/2‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TTTCTTGATCTTCTGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCAGATCCACGCCCTGCA  >  NC_002947/1049048‑1049340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑