Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,143,873 T→C 7.8% W335R (TGG→CGG)  arcD‑II → arginine/ornithine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,143,8730TC 7.8% 146.3 / 2.1 52W335R (TGG→CGG) arcD‑IIarginine/ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base C (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GCCTCGGCCTGGCGGTCTCGCTGCTTGGCGCCTTGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTATGCCGTGCTGCTGGCGATGCGCG  >  NC_002947/1143727‑1144020
                                                                                                                                                  |                                                                                                                                                   
gCCTCGGCCTGGCGGTCTCGCTGCTTGGCGCCTTGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGc                                                                                                                                                  <  2:834162/150‑1 (MQ=255)
 ccTCGGCCTGGCGGTCTCGCTGCTTGGCGCCTTGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCt                                                                                                                                                 >  1:679876/1‑150 (MQ=255)
   tCGGCCTGGCGGTCTCGCTGCTTGGCGCCTTGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGa                                                                                                                                               >  2:781102/1‑150 (MQ=255)
            cGGTCTCGCTGCTTGGCGCCTTGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCa                                                                                                                                      <  2:679876/150‑1 (MQ=255)
             ggTCTCGCTGCTTGGCGCCTTGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCAt                                                                                                                                     <  1:367037/150‑1 (MQ=255)
                 tcGCTGCTTGGCGCCTTGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGAtt                                                                                                                                 >  1:635952/1‑150 (MQ=255)
                    ctgctTGGCGCCTTGCTTTCATGGGCAATTCTGCGAGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCACGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAg                                                                                                                              <  2:534773/150‑1 (MQ=255)
                             gcCTTGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCctg                                                                                                                     <  1:455851/150‑1 (MQ=255)
                                ttGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCctgctg                                                                                                                  >  2:631247/1‑150 (MQ=255)
                                   cTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAg                                                                                                                              <  1:62409/135‑1 (MQ=255)
                                   cTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAg                                                                                                                              >  2:62409/1‑135 (MQ=255)
                                      tCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACg                                                                                                            >  1:845157/1‑150 (MQ=255)
                                        ttGGGTGCTGTTGTTTGCCGAAATCCTTTACGCCATGGCGCGGTACAAGACCATGCCGCGTTTTCTGGCCAAGGAAAACGCTAATCATGTGCCGGCTAACGCCTTGTGGTTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCt                                                                                                          <  2:211331/149‑1 (MQ=255)
                                        aTGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACGAGACCATGCCGCGCTTTCTGGACAAGGACAACGCCCACCATGGGCTGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCt                                                                                                          >  2:844183/1‑150 (MQ=255)
                                              gctgctGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCGGGGTTTCCTGCTGATTACGCTGTtctc                                                                                                    <  1:781102/150‑1 (MQ=255)
                                                tgctgTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAAc                                                                                                                                          >  2:693663/1‑110 (MQ=255)
                                                tgctgTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAAc                                                                                                                                          <  1:693663/110‑1 (MQ=255)
                                                tgctgTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTc                                                                                                                        <  2:729781/128‑1 (MQ=255)
                                                tgctgTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTc                                                                                                                        >  1:729781/1‑128 (MQ=255)
                                                          cGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGCGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCt                                                                                                          <  1:343329/132‑1 (MQ=255)
                                                          cGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGCGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCt                                                                                                          >  2:343329/1‑132 (MQ=255)
                                                          cGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGCGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCt                                                                                                          <  1:343290/132‑1 (MQ=255)
                                                          cGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGCGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCt                                                                                                          >  2:343290/1‑132 (MQ=255)
                                                              aTCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGa                                                                                                                 >  1:97948/1‑121 (MQ=255)
                                                              aTCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGa                                                                                                                 <  2:97948/121‑1 (MQ=255)
                                                                 cTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGt                                                                                 >  1:702502/1‑150 (MQ=255)
                                                                          aCGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCt                                                                                                          >  1:840776/1‑116 (MQ=255)
                                                                          aCGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCt                                                                                                          <  2:840776/116‑1 (MQ=255)
                                                                          aCGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTAc                                                                        <  2:845157/150‑1 (MQ=255)
                                                                              cgcgGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTgg                                                                    <  1:308124/150‑1 (MQ=255)
                                                                                   gACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGAAACAGTGCATGAGTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCg                                                               >  1:530946/1‑150 (MQ=255)
                                                                                    aCAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACg                                                                                                            >  1:350576/1‑104 (MQ=255)
                                                                                    aCAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACg                                                                                                            <  2:350576/104‑1 (MQ=255)
                                                                                    aCAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGt                                                              <  1:36264/150‑1 (MQ=255)
                                                                                      aaGACCATGCCGCGCTTTCTGGCCAAGGACAACGCCAACCATGTGCCGGCTAACGCCCTGGGGCTGACCAACTGCAGGATTCAGGGTTTCCTGCTGATTACGCTGTTCAATATCAGGACGTACACCAGTCTGATCTCCCTGGCCTCGTCa                                                            >  1:225482/1‑150 (MQ=255)
                                                                                         aCCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATg                                                         >  1:355782/1‑150 (MQ=255)
                                                                                           cATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGAt                                                       >  1:628154/1‑150 (MQ=255)
                                                                                           cATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGAt                                                       >  2:706340/1‑150 (MQ=255)
                                                                                               ccGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGa                                                                                                                                   >  2:4707/1‑70 (MQ=255)
                                                                                               ccGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGa                                                                                                                                   <  1:4707/70‑1 (MQ=255)
                                                                                                              aaGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTgg                                    <  1:161548/150‑1 (MQ=255)
                                                                                                              aaGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGATTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTgg                                    >  2:658187/1‑150 (MQ=255)
                                                                                                                       gCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGcc                           >  2:352665/1‑150 (MQ=255)
                                                                                                                          aaCCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTAt                        >  1:768629/1‑150 (MQ=255)
                                                                                                                               tgtgCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTATGCCGt                   <  2:702502/150‑1 (MQ=255)
                                                                                                                                      gCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTATGCCGTGctgctg            >  2:302255/1‑150 (MQ=255)
                                                                                                                                       cTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTATGCCGTGCTGCTgg           <  1:352665/150‑1 (MQ=255)
                                                                                                                                       cTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTATGCCGTGCTGCTgg           <  2:530946/150‑1 (MQ=255)
                                                                                                                                          aCGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATc                                                                           <  2:177234/83‑1 (MQ=255)
                                                                                                                                          aCGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATc                                                                           >  1:177234/1‑83 (MQ=255)
                                                                                                                                              ccTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTATGCCGTGCTGCTGGCGATgcg    >  2:96283/1‑150 (MQ=255)
                                                                                                                                               cTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCTGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTATGCCGTGCTGCTGGCGATgcgc   <  1:844183/150‑1 (MQ=255)
                                                                                                                                                tgtgGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTATGCCGTGCTGCTGGCGATgcgcg  >  1:815584/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCCTCGGCCTGGCGGTCTCGCTGCTTGGCGCCTTGCTTTCATGGGCGCTGCTGTGTGCCGAAATCCTTTACGCCACGGCGCGGGACAAGACCATGCCGCGCTTTCTGGCCAAGGAAAACGCCAACCATGTGCCGGCTAACGCCCTGTGGCTGACCAACTGCATGATTCAGGGTTTCCTGCTGATTACGCTGTTCTCCGCCGGTACGTACACCAGTCTGATCTACCTGGCCTCGTCAATGATCCTGGTGCCCTACCTGTGGTCTGCGGCCTATGCCGTGCTGCTGGCGATGCGCG  >  NC_002947/1143727‑1144020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑