Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,148,799 G→T 16.3% T151K (ACG→AAG)  PP_1007 ← transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,148,7990GT16.3% 107.0 / 11.9 43T151K (ACG→AAG) PP_1007transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base G (21/15);  new base T (3/4);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCC  >  NC_002947/1148654‑1148945
                                                                                                                                                 |                                                                                                                                                  
cTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAg                                                                                                                                                <  1:38311/150‑1 (MQ=255)
    gggACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAggtgg                                                                                                                                            <  1:224582/150‑1 (MQ=255)
      gACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTGGTc                                                                                                                                          <  2:534007/150‑1 (MQ=255)
           ggAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCt                                                                                                                                     <  2:337821/150‑1 (MQ=255)
             aaGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCAGGCCGACCACCTTACGGTGGTCGGcgagc                                                                                                                                   <  2:300510/150‑5 (MQ=255)
             aaGGAAGCGTCGCCCTGTCGCAGCAGGGCGACCTGGCGGTCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTGGTCGGcgagc                                                                                                                                   <  2:157749/150‑5 (MQ=255)
                    ccTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCAGAATTCGTCTTGCGCAGCACTTTTGCGCCGTCTTCCAGGTGTAGTCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCa                                                                                                                            >  2:312793/1‑150 (MQ=255)
                      tCGCCCTGTAGCAAGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGGGTTGAGCAGCACTTTTGCGACGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCCACCACCGTAAGGTGGTCCGCCTGAAGCCTCATg                                                                                                                          >  2:108427/1‑150 (MQ=255)
                       cGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATgg                                                                                                                         <  1:128124/150‑1 (MQ=255)
                       cGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATgg                                                                                                                         <  2:507893/150‑1 (MQ=255)
                         cccTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGc                                                                                                                       <  2:13948/150‑1 (MQ=255)
                                gCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTAc                                                                                                                >  1:617004/1‑150 (MQ=255)
                                      gggcgACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACgg                                                                                                          >  1:67870/1‑150 (MQ=255)
                                            ccTGGCGGCCGTACTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATa                                                                                                    >  1:173535/1‑150 (MQ=255)
                                               ggcggcCGTCCTGGCGTTAGCTGGCGAATGCAGAATTGGTGTTGAGCAGCACGTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGcc                                                                                                 >  2:226688/1‑150 (MQ=255)
                                                   gcCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGAc                                                                                             >  1:280388/1‑150 (MQ=255)
                                                         cTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACcagcag                                                                                       >  2:640061/1‑150 (MQ=255)
                                                                 cGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTg                                                                               >  1:559173/1‑150 (MQ=255)
                                                                  gCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGc                                                                              >  1:630151/1‑150 (MQ=255)
                                                                     ggCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGt                                                                           <  2:559173/150‑1 (MQ=255)
                                                                             ccGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAggg                                                                   <  2:617004/150‑1 (MQ=255)
                                                                                     gtgtTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAg                                                           >  1:231262/1‑150 (MQ=255)
                                                                                      tgtTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGt                                                          >  2:620208/1‑150 (MQ=255)
                                                                                           agcagcACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTg                                                     >  2:495482/1‑150 (MQ=255)
                                                                                             cagcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgcg                                                   >  1:806577/1‑150 (MQ=255)
                                                                                             cagcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgcg                                                   >  1:611451/1‑150 (MQ=255)
                                                                                             cagcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgcg                                                   >  2:289433/1‑150 (MQ=255)
                                                                                             cagcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgca                                                   >  1:79403/1‑149 (MQ=255)
                                                                                              agcaCTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTgcgc                                                  <  2:806577/150‑1 (MQ=255)
                                                                                                   ttttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGgcg                                             >  1:350051/1‑150 (MQ=255)
                                                                                                     ttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTGGTc                                                                                                                                          >  2:666885/1‑55 (MQ=37)
                                                                                                     ttGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCTTACGGTGGTc                                                                                                                                          <  1:666885/55‑1 (MQ=37)
                                                                                                                         aGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTtgc                       <  1:620208/150‑1 (MQ=255)
                                                                                                                             gctggcgctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTgctgct                   <  1:495482/150‑1 (MQ=255)
                                                                                                                                   gctCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCa             >  1:712744/1‑150 (MQ=255)
                                                                                                                                      ggCCGACCACCTTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCAtgt          >  2:342814/2‑150 (MQ=255)
                                                                                                                                      cGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCAtgt          <  2:280388/150‑1 (MQ=255)
                                                                                                                                          gACCACCTTACGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCAGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGt      >  2:349209/1‑150 (MQ=255)
                                                                                                                                          gACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGTTTTCCAGTGa                                                        >  1:674665/1‑100 (MQ=255)
                                                                                                                                          gACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGTTTTCCAGTGa                                                        <  2:674665/100‑1 (MQ=255)
                                                                                                                                          gACCACCGTAAGGTGGGCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCAGCGCCATGACCTGCGTTGCTGCTGGTGCCtgtcagt      <  2:214597/150‑5 (MQ=255)
                                                                                                                                           accaccGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTg     >  2:215803/1‑150 (MQ=255)
                                                                                                                                              accGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGcc  <  1:640061/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTCGGGGACCTGGAAGTAAGCCTCGCCCTGTAGCAGGCGGGCGACCTGGCGGCCGTCCTGGCGTTCGCTGGCGAATGCCGAATTGGTGTTGAGCAGCACTTTTGCGCCGTCTTCCAGTTGCAGGCGCTGGCGCTCGCCGACCACCGTAAGGTGGTCGGCCTGCAGGCGCATGGGCAGGTTACCGACGGTGAATAGCCCGACCAGCAGCAGCGCTGCGGTGGCCAGGGGTTTCCAGTGACTGCGCAGGCGCCCGCGCCATGACCTGCGTTGCTGCTGGTGCATGTGCGTGGCC  >  NC_002947/1148654‑1148945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑