Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,173,361 G→A 9.4% A224V (GCG→GTG)  PP_1028 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,173,3610GA 9.4% 113.5 / 2.0 42A224V (GCG→GTG) PP_1028LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (17/21);  new base A (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CTGTGCTCTGGCAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGCACGTGCTGAGTACCCGCCAAGGGGTG  >  NC_002947/1173221‑1173503
                                                                                                                                            |                                                                                                                                              
cTGTGCTCTGGCAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGCACAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCg                                                                                                                                       <  2:451294/150‑1 (MQ=255)
    gCTCTGGCAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGgaa                                                                                                                                   <  1:196584/150‑1 (MQ=255)
    gCTCTGGCAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGgaa                                                                                                                                   >  1:575337/1‑150 (MQ=255)
     ctctGGCAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTACGCGACAGTACCTGCGCCGCCTGCGGgaag                                                                                                                                  >  1:351909/1‑150 (MQ=255)
          gCAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAAccc                                                                                                                             >  1:501328/1‑150 (MQ=255)
           cAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCt                                                                                                                            <  2:575337/150‑1 (MQ=255)
           cAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCt                                                                                                                            <  1:292995/150‑1 (MQ=255)
           cAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCt                                                                                                                            <  1:11473/150‑1 (MQ=255)
            aaTCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTg                                                                                                                           >  1:162897/1‑150 (MQ=255)
             aTCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCACTCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGc                                                                                                                          <  1:433244/150‑1 (MQ=255)
             aTCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTTCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGc                                                                                                                          <  1:767897/150‑1 (MQ=255)
                  ccTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCa                                                                                                                     >  1:252455/1‑150 (MQ=255)
                        gcgcTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGcaca                                                                                                               >  1:57453/1‑150 (MQ=255)
                            tACGAAGCTGACTGAAGCCGGGGAAATCTAGCACAAGCACGGCTGCAACAAGTCGGCTCCAAACCCCGCCTCTATACATAGGAACAGCGGATGCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGa                                                                                                           >  1:831885/1‑150 (MQ=255)
                                          aaGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGc                                                                                             <  1:672481/150‑1 (MQ=255)
                                                        tcGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCCACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCCGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAACTAGCCCAACCCACCACGa                                                                               <  2:39212/150‑1 (MQ=255)
                                                        tcGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGa                                                                               >  1:330098/1‑150 (MQ=255)
                                                                     gcgGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGa                                                                  <  1:409352/150‑1 (MQ=255)
                                                                       ggCAACAACGCCACCCCAAACCCCGCCTGTATCGGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGc                                                                                                                          >  2:442892/1‑92 (MQ=255)
                                                                       ggCAACAACGCCACCCCAAACCCCGCCTGTATCGGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGc                                                                                                                          <  1:442892/92‑1 (MQ=255)
                                                                       ggCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGc                                                                                                                          <  1:442880/92‑1 (MQ=255)
                                                                       ggCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGc                                                                                                                          >  2:442880/1‑92 (MQ=255)
                                                                             aacGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATg                                                          >  2:517651/1‑150 (MQ=255)
                                                                               cGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGaa                                                        <  2:133694/150‑1 (MQ=255)
                                                                                     cccAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGctc                                                  >  1:720562/1‑150 (MQ=255)
                                                                                              cGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCaa                                         <  2:252455/150‑1 (MQ=255)
                                                                                              cGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCaa                                         <  1:585396/150‑1 (MQ=255)
                                                                                                   gTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCt                                    <  2:330098/150‑1 (MQ=255)
                                                                                                   gTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCt                                    >  2:732168/1‑150 (MQ=255)
                                                                                                      tCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGTAACTGATGAAGCTCAAACCACCACGATCCCGGTGCCGGACGGCAATGAAGCTCTCGTCCTTCAATTGCTCCa                                 >  1:363228/1‑150 (MQ=255)
                                                                                                         gCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGGAATGAAGCGCTCTTCCTTCAATTGCTCCAGtt                              <  2:57453/150‑1 (MQ=255)
                                                                                                           tGCAACTGCGTAGTCTTGCGCGACAGTACCTGCACCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGc                            >  2:25747/1‑150 (MQ=255)
                                                                                                           tGCAACTGCGTAGTCTTGCGCGACAGTACCTGCACCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGc                            >  2:26048/1‑150 (MQ=255)
                                                                                                                    gTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGCACGTGCTGa                   >  1:30978/1‑150 (MQ=255)
                                                                                                                    gTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGCACGTGCTGa                   <  1:506969/150‑1 (MQ=255)
                                                                                                                    gTAGTCTTGCGCGACAGTACCTGCACCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGCACGTGCTGa                   <  1:26048/150‑1 (MQ=255)
                                                                                                                    gTAGTCTTGCGCGACAGTACCTGCACCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGCACGTGCTGa                   <  1:25747/150‑1 (MQ=255)
                                                                                                                        tCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGaa                                                        <  1:286900/109‑1 (MQ=255)
                                                                                                                        tCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGaa                                                        >  2:286900/1‑109 (MQ=255)
                                                                                                                            gcgcGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGCACATGAAGCGCTCTTCCTTCCATTCCTCCAGTTGCACGTGCTGAGTACCCGc           <  2:162897/150‑1 (MQ=255)
                                                                                                                                gACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGCACGTGCTGAGTACCCGCCAAg       >  1:710285/1‑150 (MQ=255)
                                                                                                                                     tACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGCACGTGCTGAGTACCCGCCAAGGGgtg  <  2:501328/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                              
CTGTGCTCTGGCAATCACCCTCCAGCGCTACGAAGCTGACTGAAGCCGGGGCAATCTCGCACATGCACCGCGGCAACAACGCCACCCCAAACCCCGCCTGTATCAGCTGCAACTGCGTAGTCTTGCGCGACAGTACCTGCGCCGCCTGCGGGAAGAACCCTGCCTCCATGCACAACGAGGCAGACAAGTAGCTCAAACCACCACGATCCCGGTGCGGGATGGAAATGAAGCGCTCTTCCTTCAATTGCTCCAGTTGCACGTGCTGAGTACCCGCCAAGGGGTG  >  NC_002947/1173221‑1173503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑