Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,195,101 G→C 10.8% A412P (GCA→CCA)  uxpA → lipoprotein UxpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,195,1010GC10.8% 119.1 / 8.5 49A412P (GCA→CCA) uxpAlipoprotein UxpA
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base C (2/3);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.07e-01

CTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCGCTGCTGCTGG  >  NC_002947/1194954‑1195245
                                                                                                                                                   |                                                                                                                                                
cTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCa                                                                                                                                                >  2:771778/1‑150 (MQ=255)
 tCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAg                                                                                                                                               >  1:113168/1‑150 (MQ=255)
    aCCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTTGCGGTAGCTGGGACTGCGTGCTGAATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCgg                                                                                                                                            <  1:104661/150‑1 (MQ=255)
    aCCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCgg                                                                                                                                            <  1:797191/150‑1 (MQ=255)
     ccAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCggg                                                                                                                                           <  1:836002/150‑1 (MQ=255)
       aGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGCCAGCgggta                                                                                                                                         <  2:155614/150‑3 (MQ=255)
       aGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGCCAGCgggta                                                                                                                                         <  1:732921/150‑3 (MQ=255)
         ccGCTGGCTCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAACAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGggcg                                                                                                                                       >  1:168870/1‑150 (MQ=255)
             tGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCa                                                                                                                                   >  1:467914/1‑150 (MQ=255)
              ggCCCGCGCGCCCCAGGCCTTATGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTAGCAGCGGGGGCGGCCAt                                                                                                                                  <  2:439923/150‑1 (MQ=255)
                        ccccAGGCCTTGTGGCGCCGCCATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGaa                                                                                                                        <  1:211570/150‑1 (MQ=255)
                           cAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGCGAGGTCATTACCCGCGAACAt                                                                                                                     <  2:545038/150‑1 (MQ=255)
                            aGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATc                                                                                                                    >  2:39171/1‑150 (MQ=255)
                            aGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATc                                                                                                                    <  2:12858/150‑1 (MQ=255)
                                cTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGAt                                                                                                                >  2:394291/1‑150 (MQ=255)
                                    tgGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGc                                                                                                            <  2:467914/150‑1 (MQ=255)
                                              aTACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAg                                                                                                  <  2:113168/150‑1 (MQ=255)
                                                               tGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTg                                                                                 >  1:598702/1‑150 (MQ=255)
                                                                  gcctgcctgcAGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTc                                                                              >  2:612966/3‑150 (MQ=255)
                                                                         tgctgcATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCAc                                                                       <  2:510079/150‑1 (MQ=255)
                                                                           ctgcATCAAGCCTTTGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGCGCTTGCGTTATGACTACCCGCTGGCAGCGCGGGCGGACATTACCCGCGAACATCTGATCAGCCTTCCCGGAGGCTTCCCCGCGCCAGTGGTCGAGGCAccg                                                                     <  2:352758/150‑1 (MQ=255)
                                                                            tgcATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCAccgg                                                                    >  1:823856/1‑150 (MQ=255)
                                                                               aTCAAGCCTTGGCCGACGATAGCAGCATGCCCGCGTTGATCGAAGCACTGCGTTCGGGCTTCCCGCTGACAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCAccggccc                                                                 >  1:233535/1‑150 (MQ=255)
                                                                                tCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGggcgg                                                                                                                                      <  1:364759/80‑1 (MQ=255)
                                                                                tCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGggcgg                                                                                                                                      >  2:364759/1‑80 (MQ=255)
                                                                                tCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCAccggcccg                                                                >  1:544308/1‑150 (MQ=255)
                                                                                      cTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGt                                                          >  2:202653/1‑150 (MQ=255)
                                                                                      cTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGt                                                          <  2:598702/150‑1 (MQ=255)
                                                                                                       gcaTGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCCAGTGCTGGaaa                                         >  1:268769/1‑150 (MQ=255)
                                                                                                        caTGCCCGTGTTGTTGCCGGGCTGTCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCAGAATTCGTCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGaaaa                                        <  1:612966/150‑1 (MQ=255)
                                                                                                                     ctgcCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCaa                           >  1:196968/1‑150 (MQ=255)
                                                                                                                     ctgcCGGGCTTGCGTGATGACTACCCGCTGACAGCGGGGGCGGCCATTACCCGCAAACATCTGGTCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCTGGGCAGGTTGAGCAAGCGCTGGAAAACGCCGCTGAGCag                           >  2:139712/1‑149 (MQ=255)
                                                                                                                      tgcCGGGCTTGCGTTATGACTACCCGCTGGCATCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAAt                          >  1:295371/1‑150 (MQ=255)
                                                                                                                       gcCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAAtt                         <  2:233535/150‑1 (MQ=255)
                                                                                                                       gcCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAAtt                         >  1:805202/1‑150 (MQ=255)
                                                                                                                           ggCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTc                     <  1:771778/150‑1 (MQ=255)
                                                                                                                            gCTTGCGTTATGCCTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTAATCAGCCTTACCGGAGGCTCCCGCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCg                    <  2:106989/150‑1 (MQ=255)
                                                                                                                            gCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCg                    >  1:49545/1‑150 (MQ=255)
                                                                                                                            gCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCg                    <  1:394291/150‑1 (MQ=255)
                                                                                                                               tGCTTTATGACTCCCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCggcc                 <  2:474537/150‑3 (MQ=255)
                                                                                                                                    tATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATc                                                                                                                    <  2:639825/46‑1 (MQ=255)
                                                                                                                                    tATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATc                                                                                                                    >  1:639825/1‑46 (MQ=255)
                                                                                                                                     aTGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACAGCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCgc           >  2:617996/1‑150 (MQ=255)
                                                                                                                                       ccgcccccGCTGCCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCgctg         >  2:235979/6‑150 (MQ=255)
                                                                                                                                         gcccccGCTGCCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTc                     <  1:698098/133‑1 (MQ=255)
                                                                                                                                         gcccccGCTGCCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTc                     >  2:698098/4‑136 (MQ=255)
                                                                                                                                            cccGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCgctgctgct    >  2:324618/1‑150 (MQ=255)
                                                                                                                                             ccGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCGctgctgctg   >  1:317107/1‑150 (MQ=255)
                                                                                                                                              cGCTGGCAGCGGTGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCGCTGCTGCTgg  <  1:229477/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCGCTGCTGCTGG  >  NC_002947/1194954‑1195245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑