Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,202,863 G→T 9.8% R229L (CGG→CTG)  xcpY → type II secretion pathway protein XcpY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,202,8630GT 9.8% 123.7 / 3.5 41R229L (CGG→CTG) xcpYtype II secretion pathway protein XcpY
Reads supporting (aligned to +/- strand):  ref base G (20/17);  new base T (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

CGCAGCCACCGTGGGCAGTGCTGCACAGGACGTCGCTGAGCGGCAATTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCC  >  NC_002947/1202719‑1203005
                                                                                                                                                |                                                                                                                                              
cGCAGCCACCGTGGGCAGTGCTGCACAGGACGTCGCTGAGCGGCAATTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGgctgc                                                                                                                                           <  1:849482/150‑1 (MQ=255)
 gCAGCCACCGTGGGCAGTGCTGCACAGGACGTCGCTGAGCGGCAATTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCTCGCCAGCAGCAGCGgctgct                                                                                                                                          >  1:142744/1‑150 (MQ=255)
       aCCGTGGGCAGTGCTGCACAGGACGTCGCTGAGCGGCAATTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGTTTTGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGc                                                                                                                                    >  1:270082/1‑150 (MQ=255)
         cGTGGGCAGTGCTGCACAGGACGTCGCTGAGCGGCAATTGGCCAACCGTATTTGCGCCGAACGACCCACCATGCGCCGTGTTCGACCGCTCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCTGCCAGCAGCCGCTGCTGCTGGcgggag                                                                                                                                  <  1:104351/150‑6 (MQ=255)
              gCAGTGCTGCACAGGACGTCGCTGAGCGGCAATTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTg                                                                                                                             <  1:303675/150‑1 (MQ=255)
                               gTCGCTGAGCGGCAATTGGCCAACCGTATTTGCGCCGCTCGACACACTTGCCGGCCTGTTCGACCGCGCCCGCAAAGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGttgg                                                                                                            >  1:394293/1‑148 (MQ=255)
                                    tGAGCGGCAATTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTg                                                                                                       <  1:334428/150‑1 (MQ=255)
                                         ggCAATTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCtg                                                                                                  <  2:448139/150‑1 (MQ=255)
                                              ttGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGtt                                                                                             <  2:351586/150‑1 (MQ=255)
                                                  ccAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGt                                                                                         >  1:153483/1‑150 (MQ=255)
                                                      ccGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGGGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGc                                                                                     >  2:618381/1‑150 (MQ=255)
                                                                   gcccGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGGCTGTGGTTGAGTCAGCAATGGCGTCAGgc                                                                        <  1:301834/147‑1 (MQ=255)
                                                                           cacTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAAttct                                                                <  2:504777/150‑1 (MQ=255)
                                                                            acTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTg                                                                                                       >  2:273113/1‑110 (MQ=255)
                                                                            acTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTg                                                                                                       <  1:273113/110‑1 (MQ=255)
                                                                            acTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTg                                                               >  1:308590/1‑150 (MQ=255)
                                                                            acTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTg                                                               >  1:494738/1‑150 (MQ=255)
                                                                              tgcctcgCCTGTTTGACCGCGCCCGCTAGGTGCGCTCGTTGCCGGCAGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTCCCGTCTGGGGTGGCCTCTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGgc                                                             <  2:393384/144‑1 (MQ=255)
                                                                              tGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCTTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGgc                                                             <  1:109565/150‑1 (MQ=255)
                                                                                             aCCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTg                                              >  1:504862/1‑150 (MQ=255)
                                                                                               cgcgCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGcc                                            <  2:308590/150‑1 (MQ=255)
                                                                                                 cgcCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCTCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTCGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGt                                          <  2:142744/150‑1 (MQ=255)
                                                                                                    ccGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGAc                                       >  1:507138/1‑150 (MQ=255)
                                                                                                        aaGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCggg                                   <  2:153483/150‑1 (MQ=255)
                                                                                                          ggTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGa                                 >  1:442515/1‑150 (MQ=255)
                                                                                                                cTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGc                           >  1:335863/1‑150 (MQ=255)
                                                                                                                 tCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGcc                          >  1:687346/1‑150 (MQ=255)
                                                                                                                 tCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGcc                          >  1:311792/1‑150 (MQ=255)
                                                                                                                  cGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCa                         <  2:443758/150‑1 (MQ=255)
                                                                                                                     tGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGcc                      <  2:442515/150‑1 (MQ=255)
                                                                                                                          gccgccTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCgg                 >  1:536542/1‑150 (MQ=255)
                                                                                                                            cgccTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCggg                                   >  2:548214/1‑130 (MQ=255)
                                                                                                                            cgccTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCggg                                   <  1:548214/130‑1 (MQ=255)
                                                                                                                              ccTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCAcg             >  1:460222/1‑150 (MQ=255)
                                                                                                                               cTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCAcgc            >  2:565018/1‑150 (MQ=255)
                                                                                                                                tCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGAc                                       >  2:421570/1‑122 (MQ=255)
                                                                                                                                tCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGAc                                       <  1:421570/122‑1 (MQ=255)
                                                                                                                                  ccGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCGCGGCACGCGGc         >  1:770811/1‑150 (MQ=255)
                                                                                                                                   tgCCAGCAGCCGCTGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGca        >  1:73281/2‑150 (MQ=255)
                                                                                                                                     ccAGCAACCGCTGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGcaca      >  2:363170/1‑150 (MQ=255)
                                                                                                                                         cagccgcTGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGcc  <  2:73281/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CGCAGCCACCGTGGGCAGTGCTGCACAGGACGTCGCTGAGCGGCAATTGGCCAACCGTATTTGCGCCGCTCGACACACTGCCCGGCCTGTTCGACCGCGCCCGCAAGGTGCGCTCGTTGCCGGCCGCCTCCCGCCAGCAGCAGCGGCTGCTGGCAGCTTGCCTGGTGCTGGCTACCGTTTGGGGTGGCCTGTGGTTGAGTCAGCAATGGCGTCAGGCGCAACTCTGGCGCAGCCAGGTCATTGCCGTGACCGGGGAGCAGGCCAGCCCTCGGCACGCGGCACAGGCC  >  NC_002947/1202719‑1203005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑