| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,293,270 | C→A | 19.2% | A289A (GCC→GCA) | PP_1128 → | OmpA family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,293,270 | 0 | C | A | 19.2% | 54.6 / 5.0 | 26 | A289A (GCC→GCA) | PP_1128 | OmpA family protein |
| Reads supporting (aligned to +/- strand): ref base C (10/11); new base A (3/2); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
CCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGC > NC_002947/1293129‑1293413 | ccTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtgg > 1:633172/1‑150 (MQ=255) ggAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCaa < 2:233088/150‑1 (MQ=255) tGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCGGCCTTGGCAGCtgg > 2:81713/1‑127 (MQ=255) cATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt > 2:383014/1‑142 (MQ=255) cATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt < 1:383014/142‑1 (MQ=255) aTGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAg > 2:283218/1‑150 (MQ=255) tGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCCCCCCCGCCTCGATGGTGGGGGTGCAGGATCCTTACGCACCAGCGGCCAAGGCCGCTGGTGGCAAACGTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAgg > 2:524241/1‑150 (MQ=255) ggTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGc < 2:60594/150‑1 (MQ=255) ggTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGc < 1:592540/150‑1 (MQ=255) gctgctGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAAc > 1:70553/1‑150 (MQ=255) cAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGCCACCAGCGGCCTTGGCAGCtggtgcctt > 2:210967/1‑89 (MQ=25) cAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGCCACCAGCGGCCTTGGCAGCtggtgcctt < 1:210967/93‑5 (MQ=25) aaTACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGa < 1:315096/150‑1 (MQ=255) ggCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCaa < 2:70553/150‑1 (MQ=255) gccgccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCa > 1:485316/1‑144 (MQ=255) gccgccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCa < 2:485316/144‑1 (MQ=255) cGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCa > 1:156625/1‑150 (MQ=255) ggCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCaa < 2:156625/150‑1 (MQ=255) cAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGa < 1:636211/92‑1 (MQ=255) cAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGa > 2:636211/1‑92 (MQ=255) cAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGa < 1:283218/150‑1 (MQ=255) gccACCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACaa > 1:343586/3‑150 (MQ=255) aGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAgg < 1:411462/65‑1 (MQ=255) aGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAgg > 2:411462/1‑65 (MQ=255) cggccttggCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAg < 2:343586/143‑1 (MQ=255) cAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGc > 2:275290/1‑150 (MQ=255) | CCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGC > NC_002947/1293129‑1293413 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |