| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 132,467 | G→C | 11.1% | intergenic (+41/‑111) | cc → / → dsbA | cytochrome c4/thiol:disulfide interchange protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 132,467 | 0 | G | C | 11.1% | 104.0 / 5.6 | 36 | intergenic (+41/‑111) | cc/dsbA | cytochrome c4/thiol:disulfide interchange protein |
| Reads supporting (aligned to +/- strand): ref base G (16/16); new base C (2/2); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01 | |||||||||||
CCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTGGT > NC_002947/132324‑132609 | ccGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGc < 2:754761/150‑1 (MQ=255) cGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTACTGTTGGAGGTAAGGGATGAAAGGGCGGCCGGGCTGCCCTTTTTtc > 1:405009/1‑150 (MQ=255) gtGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCGACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccg > 2:585843/2‑150 (MQ=255) ccATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGcc < 2:759926/150‑1 (MQ=255) cATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACt > 2:616895/1‑150 (MQ=255) aTCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTc < 1:616895/150‑1 (MQ=255) gCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAg > 1:272132/1‑150 (MQ=255) gCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGc > 1:654434/1‑102 (MQ=255) gCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGc < 2:654434/102‑1 (MQ=255) aGCAACAATAACATCGAAGCGTTGCCCTGCTACATCTAGTGGCTGTATTAACCTTCGCTTCATGATGTAGGTCAATGATCAAAGGGCGGCCGGGCTGCCCTTTTTTACGTTCGCATCCTTTACACTTCAGAACTCCACCCCTTCCCGGcc < 2:266987/150‑1 (MQ=255) cATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGc < 2:191908/150‑1 (MQ=255) gTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTACCGGCCTGTCTCAGTGCAGGTCGcgt < 2:684206/150‑1 (MQ=255) cTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGAc > 2:453588/1‑150 (MQ=255) tCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCTTTTTTTCGGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGgagagcagta > 2:265458/1‑144 (MQ=255) tCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATg > 2:772479/1‑150 (MQ=255) ccAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGa < 2:405009/150‑1 (MQ=255) aGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCAGCCCGGCCGCCCttt > 2:791998/1‑66 (MQ=37) aGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCAGCCCGGCCGCCCttt < 1:791998/66‑1 (MQ=37) cTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGa < 2:569465/143‑1 (MQ=255) cTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGa > 1:569465/1‑143 (MQ=255) tttCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTac < 2:388521/71‑1 (MQ=255) tttCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTac > 1:388521/1‑71 (MQ=255) tttCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGc > 1:24671/1‑150 (MQ=255) tCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCAt < 1:453588/150‑1 (MQ=255) cGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATg < 1:772479/150‑1 (MQ=255) gTTAATGTGGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCg > 2:224158/1‑150 (MQ=255) gttgtAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTg < 2:178814/125‑1 (MQ=255) gttgtAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTg > 1:178814/1‑125 (MQ=255) ttgtAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAATGCAGGTCGCGTCGTGGCGACCCATGACTACTCAGGAGTAAAGCATGCGTAAACTg > 2:600823/1‑150 (MQ=255) cAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAg < 2:272132/150‑1 (MQ=255) aaTGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAgc > 2:576678/1‑150 (MQ=255) gaAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGc > 1:265824/1‑127 (MQ=255) gaAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGc < 2:265824/127‑1 (MQ=255) aaaaGGGCAGCCCGGCCGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCa < 2:174035/112‑1 (MQ=255) aaaaGGGCAGCCCGGCCGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCa > 1:174035/2‑113 (MQ=255) ggcggcCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTGGt < 1:485268/150‑1 (MQ=255) | CCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTGGT > NC_002947/132324‑132609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |