Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,356,411 G→C 24.3% G94A (GGC→GCC)  PP_1181 → two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,356,4110GC24.3% 56.6 / 19.0 33G94A (GGC→GCC) PP_1181two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (16/9);  new base C (4/4);  total (20/13)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

CTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTAT  >  NC_002947/1356284‑1356549
                                                                                                                               |                                                                                                                                          
cTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCTCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCgacgac                                                                                                                      <  1:332281/150‑1 (MQ=255)
             aCCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAAc                                                                                                         >  2:128666/1‑150 (MQ=255)
                  aTGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTg                                                                                                    >  2:364853/1‑150 (MQ=255)
                    gCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTgg                                                                                                                                 <  1:197102/119‑1 (MQ=255)
                    gCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTgg                                                                                                                                 >  2:197102/1‑119 (MQ=255)
                             cGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCtt                                                                                         <  1:451876/150‑1 (MQ=255)
                                       cTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCa                                                                               >  2:154981/1‑150 (MQ=255)
                                              tCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATcc                                                                        >  2:41979/1‑150 (MQ=255)
                                                   cgccgGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGcc                                                                   >  2:499004/1‑150 (MQ=255)
                                                     ccgGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCct                                                                 >  1:102835/1‑150 (MQ=255)
                                                          tGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCtgctgc                                                            <  2:816207/150‑1 (MQ=255)
                                                                               cGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACa                                       <  1:499004/150‑1 (MQ=255)
                                                                               cGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACa                                       >  1:99792/1‑150 (MQ=255)
                                                                                          cTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAg                            <  2:99792/150‑1 (MQ=255)
                                                                                          cTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTACAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCCCCAg                            >  2:453056/1‑150 (MQ=255)
                                                                                             aCCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCATCGAAACCAGGGACAGCAAGCGCCAGGAc                         >  2:45005/1‑150 (MQ=255)
                                                                                             aCCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGAc                         >  1:550760/1‑150 (MQ=255)
                                                                                                 cgcgcAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTgg                     <  1:364853/150‑1 (MQ=255)
                                                                                                 cgcgcAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTgg                     <  1:41979/150‑1 (MQ=255)
                                                                                                          atgaACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCg               >  2:330118/1‑147 (MQ=255)
                                                                                                          atgaACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCg               <  1:330118/147‑1 (MQ=255)
                                                                                                          atgaACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTg            >  1:559183/1‑150 (MQ=255)
                                                                                                              aCTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCt        >  2:541121/1‑150 (MQ=255)
                                                                                                                  gACCAGTTCCCAGGCCTTGACTTGGCCTGTGCCGACTACTTCTGCAACCGCGTGCGCCCGCGCTTGCTGCCGGCACACATCCCGGCGCTGCTGCCCCGACGCGCAAACGTGGCCGGCAAGCGCCAGGACCTGGCCGTCGGTGCGCtaggt    >  2:100233/1‑146 (MQ=255)
                                                                                                                    ccAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTAt  >  2:727922/1‑150 (MQ=255)
                                                                                                                    ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTgcgc                                                          <  2:443355/94‑1 (MQ=255)
                                                                                                                    ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTgcgc                                                          >  1:443355/1‑94 (MQ=255)
                                                                                                                    ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCa                                                      <  1:204538/98‑1 (MQ=255)
                                                                                                                    ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCa                                                      >  2:204538/1‑98 (MQ=255)
                                                                                                                    ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTg                                           <  1:459810/109‑1 (MQ=255)
                                                                                                                    ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTg                                           >  2:459810/1‑109 (MQ=255)
                                                                                                                     cAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCAc                                                                              <  2:341561/73‑1 (MQ=255)
                                                                                                                     cAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCAc                                                                              >  1:341561/1‑73 (MQ=255)
                                                                                                                               |                                                                                                                                          
CTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTAT  >  NC_002947/1356284‑1356549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑