| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 141,137 | T→A | 10.2% | Q237L (CAG→CTG) | algB ← | alginate biosynthesis transcriptional regulator AlgB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 141,137 | 0 | T | A | 10.2% | 114.1 / 4.6 | 39 | Q237L (CAG→CTG) | algB | alginate biosynthesis transcriptional regulator AlgB |
| Reads supporting (aligned to +/- strand): ref base T (18/17); new base A (2/2); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
TGGCAGCCAGGATACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTCCAACCATGGAT > NC_002947/140988‑141285 | ttgCAGCCAGGATACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCt < 1:441417/148‑1 (MQ=255) cAGGATACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCAGGCTGAc > 1:592930/1‑150 (MQ=255) gATACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCAGGCTGAcggg < 2:592930/150‑4 (MQ=255) aTACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGAcccgc < 2:158270/150‑1 (MQ=255) gTACATCGGCACGCCGGGTAATGGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCAGGCTGAcggg < 1:429899/145‑4 (MQ=255) gTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACATTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGa > 2:348780/1‑150 (MQ=255) gTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCAGGCTGAcggg > 2:429899/1‑142 (MQ=255) aCGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATATAGCGGAAGAATGTCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTc > 2:663237/1‑150 (MQ=255) cGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTTCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGt > 2:355643/1‑150 (MQ=255) cGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGt < 1:710895/150‑1 (MQ=255) ggTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGa > 1:305525/1‑150 (MQ=255) cGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGgcgc < 1:513919/150‑1 (MQ=255) cGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGCACTCTGGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGgcgc < 2:292725/150‑1 (MQ=255) gACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGcgcc > 2:530010/1‑150 (MQ=255) gTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCggg > 2:678577/1‑150 (MQ=255) ttCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCgggg > 2:836911/1‑149 (MQ=255) ttCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGAcc < 1:111216/150‑1 (MQ=255) tGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATa < 2:485923/150‑1 (MQ=255) gTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAg < 1:678577/150‑1 (MQ=255) tGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCTTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCg < 1:530010/150‑1 (MQ=255) aaCGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCa > 2:308108/1‑150 (MQ=255) aCGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGa < 1:680903/80‑1 (MQ=255) aCGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGa > 2:680903/1‑80 (MQ=255) gCAACATTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCa < 1:348780/150‑1 (MQ=255) aaCAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAAt < 2:304823/132‑1 (MQ=255) aaCAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAAt > 1:304823/1‑132 (MQ=255) gTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCgg > 1:441976/1‑150 (MQ=255) tGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTACATCAGCTCGGCGTTAAGc > 1:44124/1‑150 (MQ=255) cGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGtt > 1:250150/1‑150 (MQ=255) gATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCACGCa > 1:399524/1‑150 (MQ=255) tcgAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTg > 2:560468/1‑150 (MQ=255) cgAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGc < 2:305525/150‑1 (MQ=255) gTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCa > 2:145190/1‑127 (MQ=255) gTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCa < 1:145190/127‑1 (MQ=255) tGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCAc > 2:514943/1‑127 (MQ=255) tGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCAc < 1:514943/127‑1 (MQ=255) ccccGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTcc > 2:810519/1‑150 (MQ=255) ccccGTCAGCCTGGCTGACCCGACCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTcc < 2:218373/150‑1 (MQ=255) ccTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTCCAACCatgga < 2:441976/150‑1 (MQ=255) cTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTCCAACCatggat < 1:308108/150‑1 (MQ=255) | TGGCAGCCAGGATACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCGGCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTCCAACCATGGAT > NC_002947/140988‑141285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |