| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,504,077 | T→A | 20.0% | intergenic (+35/‑228) | rpsI → / → petA | 30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,504,077 | 0 | T | A | 20.0% | 78.8 / 17.5 | 40 | intergenic (+35/‑228) | rpsI/petA | 30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit |
| Reads supporting (aligned to +/- strand): ref base T (13/19); new base A (3/5); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.20e-01 | |||||||||||
CCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTA > NC_002947/1503928‑1504217 | ccTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCtt > 2:645874/1‑150 (MQ=255) cTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTg < 1:241794/150‑1 (MQ=255) ggCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGATAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCg < 2:627325/150‑1 (MQ=255) gcgcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCgg < 1:361819/150‑1 (MQ=255) gcgcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCgg < 1:522483/150‑1 (MQ=255) gcgcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGCAGTGGAAAGCCCGGTTCCAAGGAACCgg < 1:542876/150‑1 (MQ=255) ggCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCtt < 1:84147/150‑1 (MQ=255) gCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑ttttttt > 2:381151/1‑141 (MQ=255) gCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑ttttttt < 1:381151/141‑1 (MQ=255) gCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTg > 2:751352/1‑150 (MQ=255) ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTg > 2:828366/1‑150 (MQ=255) ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTg > 2:145730/1‑150 (MQ=255) tCGTTAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGcagtc < 1:145730/150‑1 (MQ=255) gTGAAGTTGAGCGTACGAAAGGGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAACACCGCTTGGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGcagtcag < 1:683307/150‑1 (MQ=255) gTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGcc < 1:751352/150‑1 (MQ=255) gTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGcc < 2:214624/150‑1 (MQ=255) gTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTc < 1:774291/150‑1 (MQ=255) tAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCg > 2:619501/1‑150 (MQ=255) aaaGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATa < 2:508817/150‑1 (MQ=255) gTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAg < 2:307409/150‑1 (MQ=255) aaGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTgg < 1:414507/72‑1 (MQ=255) aaGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTgg > 2:414507/1‑72 (MQ=255) cgTAAGCATCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGACCAAGCAAAGCGt < 2:491388/150‑1 (MQ=255) ctCCTCAGTCCTCCAAGAGTTAATACCGCTGTGGCAGTCGAAAAAAGCCCGGTTCCTTGGATCCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGt < 1:540524/149‑1 (MQ=255) tCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCggg < 2:821000/66‑1 (MQ=37) tCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCggg > 1:821000/1‑66 (MQ=37) cTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGc > 2:795282/1‑150 (MQ=255) tCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCAGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCg > 2:328897/1‑150 (MQ=255) ttAATACCACTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATc < 1:328897/150‑1 (MQ=255) ccGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTg > 2:731958/1‑150 (MQ=255) ccGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTg > 2:281762/1‑150 (MQ=255) ccggacgcGAAAAAAGCCCGGTTCCTTTGACCCGGGCTTTTTTTTTTAGCCTTGAACTATCCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAgt < 1:279958/143‑1 (MQ=255) gggaGTCGAAAAAAGCCCGGTTCCTTGGAACCGCGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTACCGCGGTAATCACCTTGTCAgtgt < 1:161442/147‑1 (MQ=255) tCGAAAACAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGc < 2:151777/116‑1 (MQ=255) tCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGc > 1:151777/1‑116 (MQ=255) aaaGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGtt > 1:365393/1‑150 (MQ=255) cccGGTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAg > 1:666154/1‑88 (MQ=255) cccGGTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAg < 2:666154/88‑1 (MQ=255) cccGGTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCtt > 2:70834/1‑150 (MQ=255) ccGGTTCCTTGGAACCGGGC‑TTTTTTTTTATCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTa > 1:157654/1‑150 (MQ=255) ccGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTa < 1:731958/150‑1 (MQ=255) | CCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTA > NC_002947/1503928‑1504217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |