| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,515,933 | C→A | 8.1% | P276T (CCG→ACG) | ftsI → | peptidoglycan DD transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,515,933 | 0 | C | A | 8.1% | 150.8 / 2.6 | 49 | P276T (CCG→ACG) | ftsI | peptidoglycan DD transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (23/22); new base A (2/2); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
CTGGCTACCCGCGAGTTGCGCAACGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAGGTGTACCCTGGCAGCCTG > NC_002947/1515789‑1516070 | cTGGCTACCCGCGAGTTGCGCAACGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCcggcg > 1:526162/1‑150 (MQ=255) tACCCGCGAGTTGCGCAACGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCAt < 1:14383/150‑1 (MQ=255) cccGCGAGTTGCGCAACGCCATTGCCGAACAGGACGCCTGATCTGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGc < 1:437975/150‑1 (MQ=255) cccGCGAGTTGCGCAACGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGc < 1:67307/150‑1 (MQ=255) ccGCGAGTTGCGCAACGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCg > 2:354254/1‑150 (MQ=255) ccGCGAGTTGCGCAACGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCg > 1:210011/1‑150 (MQ=255) gcgAGTTGCGCAACGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAAACGACTGACCAGGTTCCCCGCGGCCATGCGTa > 2:323444/1‑150 (MQ=255) gcgcAACGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGc < 1:354254/150‑1 (MQ=255) aaCGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGc > 2:349640/1‑136 (MQ=255) aaCGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGc < 1:349640/136‑1 (MQ=255) ccATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCg > 1:848854/1‑150 (MQ=255) aTTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGAc < 2:436367/150‑1 (MQ=255) gagcAGGGCGCCAAGGGCGGCAGGCGGGTGATTATGGATGTCAAGCGCGGTGAGGTACTGGCGATGGTCAAGCAAGCGACCTACAACCCCCACGACCGACGTAGCATGTTCCCGGCGGCCCTGCGTAACCGGGCGATCATCGACGTGTTc < 2:137319/147‑1 (MQ=255) gAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTc > 1:333348/1‑150 (MQ=255) aaCATGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCggg < 2:112646/132‑1 (MQ=255) aaCATGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCggg > 1:112646/1‑132 (MQ=255) aGGACGCCAAGGCAAGCAGTCCGAAGCTAATAAATGTAAAGAACGGGTATGTCCTGGCCATTTTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGc > 2:650313/1‑150 (MQ=255) cGCCAATGCCGGCAGCCTGTTGATTATGGATGTCAAGACCGGTGAGGTCCTGGCCAGGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCg‑gg < 2:280931/150‑1 (MQ=255) tGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCATCAGCCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGTTCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGa < 1:323444/150‑1 (MQ=255) aTGGATGTCAAGACCGGTGGGGTCCTGGCGATGGTCAACCAACCGACCTACAACCCCAACAACCGACATAGCATGTTACCGGCGGCCATGCGTAACCGAGCGATCATCGACGTGTTCGAGCCG‑TGCTGCACGGTCAAGCCGATCTCCATg > 1:298717/1‑150 (MQ=255) aTGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATg > 2:737675/1‑150 (MQ=255) tGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGa < 1:632853/150‑1 (MQ=255) tGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGa > 1:406967/1‑150 (MQ=255) tGGATCTCAAGCCCGCTCAAGTCCTGGCAAAGGTGCAACAAGCGAGCTATATCCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGa < 2:225429/150‑1 (MQ=255) aaGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTC‑ACCGGTGGCTCCACGGTCAAGCCGATCTCCATGAGTgcggcg > 1:437637/1‑150 (MQ=255) gTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGc < 1:480876/75‑1 (MQ=255) gTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGc > 2:480876/1‑75 (MQ=255) tGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCagag > 2:305275/1‑150 (MQ=255) tGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTAGAGCCG‑GACTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCagca > 1:91459/1‑148 (MQ=255) gTCAACCAACCGATCTATAACCCCCACCACCGCGGTAGTATGTTCCCGGCGGCCACGCGTAACCGGGCGATCATGCACGTCGGCGAGCCG‑GGCTCTGGGGTCCACCCGTTCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGccg < 2:210011/150‑1 (MQ=255) aaccaaccGACCTACAACCCCAACAACCGACGTAGCATGTTCACGGCGGCCATGc < 1:471878/55‑1 (MQ=255) aaccaaccGACCTACAACCCCAACAACCGACGTAGCATGTTCACGGCGGCCATGc > 2:471690/1‑55 (MQ=255) aaccaaccGACCTACAACCCCAACAACCGACGTAGCATGTTCACGGCGGCCATGc > 2:471878/1‑55 (MQ=255) aaccaaccGACCTACAACCCCAACAACCGACGTAGCATGTTCACGGCGGCCATGc < 1:471690/55‑1 (MQ=255) accaaccGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGccgaccg > 1:340538/1‑150 (MQ=255) ccaaccGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGccgaccga > 1:833046/1‑150 (MQ=255) accGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACaa > 1:283075/1‑150 (MQ=255) gACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGt > 1:292226/1‑150 (MQ=255) cTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGa < 2:283075/150‑1 (MQ=255) tACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAg < 2:848854/150‑1 (MQ=255) cAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAGgt < 2:406967/150‑1 (MQ=255) caacCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGGGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAGGTGTACCCTgg < 2:333348/150‑1 (MQ=255) aacCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGaa < 2:257623/119‑1 (MQ=255) aacCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGaa > 1:257623/1‑119 (MQ=255) cGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAGGTGTACCCTGGCAGc > 1:547207/1‑150 (MQ=255) cGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAGGTGTACCCTGGCAGc < 1:305275/150‑1 (MQ=255) cGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAGGTGTACCCTGGCAGc > 1:139217/1‑150 (MQ=255) cGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTAGCGCGC‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAGGTGTACCCTGGCAGc < 1:650313/150‑1 (MQ=255) cGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAGGTGTACCCTGGCAGCCTg < 1:737675/150‑1 (MQ=255) | CTGGCTACCCGCGAGTTGCGCAACGCCATTGCCGAACAGGACGCCAAGGCCGGCAGCCTGGTGATCATGGATGTCAAGACCGGTGAGGTCCTGGCCATGGTCAACCAACCGACCTACAACCCCAACAACCGACGTAGCATGTTCCCGGCGGCCATGCGTAACCGGGCGATCATCGACGTGTTCGAGCCG‑GGCTCCACGGTCAAGCCGATCTCCATGAGTGCGGCGCTGCAGAGTGGCCGCTGGAAGCCGACCGACAAGGTCGAGGTGTACCCTGGCAGCCTG > NC_002947/1515789‑1516070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |