Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,553,252 A→C 15.0% E84A (GAG→GCG)  PP_1363 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,553,2520AC15.0% 78.4 / 11.9 40E84A (GAG→GCG) PP_1363hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (20/14);  new base C (3/3);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.25e-01

CATGGACCACCATCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTGG  >  NC_002947/1553103‑1553398
                                                                                                                                                     |                                                                                                                                                  
cATGGACCACCATCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCCGGTTGATCGCTCCAGCCGTCGCCGGCGTGAGATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGa                                                                                                                                                    <  1:445096/150‑1 (MQ=255)
        accaTCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAgg                                                                                                                                            >  1:561692/1‑150 (MQ=255)
            tCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGtt                                                                                                                                        >  2:241923/1‑150 (MQ=255)
              tGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTCCAGGcac                                                                                                                                         >  2:670843/1‑145 (MQ=255)
              tGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTCCAGGcac                                                                                                                                         <  1:670843/147‑3 (MQ=255)
              tGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGt                                                                                                                                      >  1:675389/1‑150 (MQ=255)
                      gCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGAc                                                                                                                              <  2:198317/150‑1 (MQ=255)
                        cTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGATCGCCAGGCGTTGTACGTGGAcgc                                                                                                                            >  2:263368/1‑150 (MQ=255)
                         tACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGAcgcg                                                                                                                           >  2:832012/1‑150 (MQ=255)
                           ccGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACgcggc                                                                                                                         <  1:471318/150‑1 (MQ=255)
                              cgcCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTCCAGGcactg                                                                                                                                       <  2:844378/133‑5 (MQ=255)
                              cgcCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTCCAGGcactg                                                                                                                                       >  1:844378/1‑129 (MQ=255)
                                           ggcgATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTAc                                                                                                         >  2:660114/1‑150 (MQ=255)
                                               aTTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGGGAGGCGTTGTACGTGGACGCGGCGCAAACGTATCAGTACCttg                                                                                                     >  1:398858/1‑149 (MQ=255)
                                                    gcACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcag                                                                                                <  1:707024/150‑1 (MQ=255)
                                                    gcACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcag                                                                                                <  2:675389/150‑1 (MQ=255)
                                                      aCGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcagca                                                                                              >  2:804523/1‑150 (MQ=255)
                                                                cTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGc                                                                                    >  2:818930/1‑150 (MQ=255)
                                                                 tCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCa                                                                                   >  2:799152/1‑150 (MQ=255)
                                                                       tGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGC‑GCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTAt                                                                                                               <  1:785989/115‑1 (MQ=255)
                                                                       tGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGC‑GCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTAt                                                                                                               >  2:785989/1‑115 (MQ=255)
                                                                         tCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAatat                                                                           <  1:799152/150‑1 (MQ=255)
                                                                           gCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGc                                                                         >  1:430945/1‑150 (MQ=255)
                                                                               gTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGTGGCGCAAACCTATCa                                                                                                             >  1:837514/1‑110 (MQ=255)
                                                                               gTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGTGGCGCAAACCTATCa                                                                                                             <  2:837514/110‑1 (MQ=255)
                                                                                  gATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGgcgc                                                                                                                       <  1:429925/97‑1 (MQ=255)
                                                                                  gATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGgcgc                                                                                                                       >  2:429925/1‑97 (MQ=255)
                                                                                       cACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCg                                                             <  2:117295/150‑1 (MQ=255)
                                                                                         ccAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCggg                                                           >  2:13454/1‑150 (MQ=255)
                                                                                                         gTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCt                                           <  1:13454/150‑1 (MQ=255)
                                                                                                                ccccgcGAAGTGTCACTGCTGCGCGGCCAGGGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGaaaaa                                    <  2:405612/145‑1 (MQ=255)
                                                                                                                 aCCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTGTCGGGCCCGGGCGCGCCTGCAGACCCTGGAAacac                                   >  1:728653/1‑150 (MQ=255)
                                                                                                                   cccGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcag                                                                                                >  2:282141/1‑87 (MQ=255)
                                                                                                                   cccGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcag                                                                                                <  1:282141/87‑1 (MQ=255)
                                                                                                                   cccGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTc                                 >  1:466971/1‑150 (MQ=255)
                                                                                                                      gAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCg                                >  2:48512/1‑148 (MQ=255)
                                                                                                                      gAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCg                                <  1:48512/148‑1 (MQ=255)
                                                                                                                                     cgcgGCCCGTGCCTGGAGCGCGAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGCCCCTGGAAAAACTCGGTCATTTGCGCGGTGCa               <  2:151377/150‑1 (MQ=255)
                                                                                                                                            aacgCCTGGCGCTCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTAACCGGGCAACGAat                                                                             >  2:42964/4‑81 (MQ=255)
                                                                                                                                            aacgCCTGGCGCTCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTAACCGGGCAACGAat                                                                             <  1:42964/78‑1 (MQ=255)
                                                                                                                                                  tGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTgg  >  1:625448/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CATGGACCACCATCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTGG  >  NC_002947/1553103‑1553398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑