Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,553,255 G→T 15.0% R85L (CGC→CTC)  PP_1363 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,553,2550GT15.0% 95.6 / 9.3 40R85L (CGC→CTC) PP_1363hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/14);  new base T (3/3);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-01

ACCATCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTGG  >  NC_002947/1553111‑1553398
                                                                                                                                                |                                                                                                                                               
accaTCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAgg                                                                                                                                            >  1:561692/1‑150 (MQ=255)
    tCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGtt                                                                                                                                        >  2:241923/1‑150 (MQ=255)
      tGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTCCAGGcac                                                                                                                                         >  2:670843/1‑145 (MQ=255)
      tGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTCCAGGcac                                                                                                                                         <  1:670843/147‑3 (MQ=255)
      tGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGt                                                                                                                                      >  1:675389/1‑150 (MQ=255)
              gCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGAc                                                                                                                              <  2:198317/150‑1 (MQ=255)
                cTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGATCGCCAGGCGTTGTACGTGGAcgc                                                                                                                            >  2:263368/1‑150 (MQ=255)
                 tACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGAcgcg                                                                                                                           >  2:832012/1‑150 (MQ=255)
                   ccGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACgcggc                                                                                                                         <  1:471318/150‑1 (MQ=255)
                      cgcCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTCCAGGcactg                                                                                                                                       >  1:844378/1‑129 (MQ=255)
                      cgcCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTCCAGGcactg                                                                                                                                       <  2:844378/133‑5 (MQ=255)
                                   ggcgATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTAc                                                                                                         >  2:660114/1‑150 (MQ=255)
                                       aTTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGGGAGGCGTTGTACGTGGACGCGGCGCAAACGTATCAGTACCttg                                                                                                     >  1:398858/1‑149 (MQ=255)
                                            gcACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcag                                                                                                <  1:707024/150‑1 (MQ=255)
                                            gcACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcag                                                                                                <  2:675389/150‑1 (MQ=255)
                                              aCGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcagca                                                                                              >  2:804523/1‑150 (MQ=255)
                                                        cTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGc                                                                                    >  2:818930/1‑150 (MQ=255)
                                                         tCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCa                                                                                   >  2:799152/1‑150 (MQ=255)
                                                               tGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGC‑GCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTAt                                                                                                               >  2:785989/1‑115 (MQ=255)
                                                               tGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGC‑GCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTAt                                                                                                               <  1:785989/115‑1 (MQ=255)
                                                                 tCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAatat                                                                           <  1:799152/150‑1 (MQ=255)
                                                                   gCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGc                                                                         >  1:430945/1‑150 (MQ=255)
                                                                       gTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGTGGCGCAAACCTATCa                                                                                                             >  1:837514/1‑110 (MQ=255)
                                                                       gTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGTGGCGCAAACCTATCa                                                                                                             <  2:837514/110‑1 (MQ=255)
                                                                          gATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGgcgc                                                                                                                       <  1:429925/97‑1 (MQ=255)
                                                                          gATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGgcgc                                                                                                                       >  2:429925/1‑97 (MQ=255)
                                                                               cACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCg                                                             <  2:117295/150‑1 (MQ=255)
                                                                                 ccAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCggg                                                           >  2:13454/1‑150 (MQ=255)
                                                                                                 gTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCt                                           <  1:13454/150‑1 (MQ=255)
                                                                                                        ccccgcGAAGTGTCACTGCTGCGCGGCCAGGGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGaaaaa                                    <  2:405612/145‑1 (MQ=255)
                                                                                                         aCCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTGTCGGGCCCGGGCGCGCCTGCAGACCCTGGAAacac                                   >  1:728653/1‑150 (MQ=255)
                                                                                                           cccGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcag                                                                                                >  2:282141/1‑87 (MQ=255)
                                                                                                           cccGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCcag                                                                                                <  1:282141/87‑1 (MQ=255)
                                                                                                           cccGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTc                                 >  1:466971/1‑150 (MQ=255)
                                                                                                              gAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCg                                >  2:48512/1‑148 (MQ=255)
                                                                                                              gAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCg                                <  1:48512/148‑1 (MQ=255)
                                                                                                                             cgcgGCCCGTGCCTGGAGCGCGAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGCCCCTGGAAAAACTCGGTCATTTGCGCGGTGCa               <  2:151377/150‑1 (MQ=255)
                                                                                                                                    aacgCCTGGCGCTCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTAACCGGGCAACGAat                                                                             >  2:42964/4‑81 (MQ=255)
                                                                                                                                    aacgCCTGGCGCTCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTAACCGGGCAACGAat                                                                             <  1:42964/78‑1 (MQ=255)
                                                                                                                                          tGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTgg  >  1:625448/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ACCATCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTGG  >  NC_002947/1553111‑1553398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑