Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,625,250 A→C 10.3% intergenic (+31/+26) PP_1425 → / ← nadB hypothetical protein/L‑aspartate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,625,2500AC10.3% 94.5 / 4.5 39intergenic (+31/+26)PP_1425/nadBhypothetical protein/L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base A (15/20);  new base C (2/2);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

GTTGGCTGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGC  >  NC_002947/1625101‑1625399
                                                                                                                                                     |                                                                                                                                                     
gTTGGCTGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGa                                                                                                                                                       <  2:644262/150‑1 (MQ=255)
    gCTGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGccc                                                                                                                                                   <  2:817624/150‑1 (MQ=255)
         gggAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGGCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGGCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGaa                                                                                                                                              <  1:839240/150‑1 (MQ=255)
            aaaGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCTCGCCCGCGAAgaa                                                                                                                                           <  1:361893/150‑3 (MQ=255)
            aaaGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGc                                                                                                                                           >  2:555348/1‑150 (MQ=255)
                    tgtATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGc                                                                                                                                   <  2:624010/150‑1 (MQ=255)
                           cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGaa                                                                                                                                              >  1:41382/1‑132 (MQ=255)
                           cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGaa                                                                                                                                              <  2:41382/132‑1 (MQ=255)
                           cTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATc                                                                                                                            >  1:676670/1‑150 (MQ=255)
                                     gcTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGtt                                                                                                                  >  2:695379/1‑150 (MQ=255)
                                        aGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTTAGCCGGGCATAGATCGAGTCCGGGTTAGCTTCTTGGGGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAg                                                                                                               <  1:32618/150‑1 (MQ=255)
                                            gctgttgAGCATCGGCGCTTGCGTTTGCGGTTGAAGGTCAGCCGGCGGCGCTGCGCGGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCTGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                           <  1:814369/144‑1 (MQ=255)
                                            gCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                           <  1:133662/150‑1 (MQ=255)
                                            gCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                           <  1:531255/150‑1 (MQ=255)
                                            gCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                           <  1:449399/150‑1 (MQ=255)
                                            gCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                           <  2:789033/150‑1 (MQ=255)
                                             cTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATgg                                                                                                          <  1:734772/150‑1 (MQ=255)
                                                       tCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGAAGTCTATATCAGAGCGGGTTAAGGATGGTGTCCTTGGc                                                                                                <  1:415957/150‑1 (MQ=255)
                                                        cGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGcc                                                                                               <  2:676670/150‑1 (MQ=255)
                                                              ttGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTcc                                                                                         >  2:482584/1‑150 (MQ=255)
                                                                  gggTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGgtgt                                                                                                       <  2:153961/132‑1 (MQ=255)
                                                                  gggTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGgtgt                                                                                                       >  1:153961/1‑132 (MQ=255)
                                                                          ttGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGGTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTggg                                                                             >  2:785850/1‑149 (MQ=255)
                                                                               gTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCGCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGCTTGATGTCCTTGGCCTCGTCCAGCATCCCTGGATAATc                                                                        <  1:285702/150‑1 (MQ=255)
                                                                                ttCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCggg                                                                                                                    >  2:362534/1‑105 (MQ=255)
                                                                                ttCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCggg                                                                                                                    <  1:362534/105‑1 (MQ=255)
                                                                                             gcTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgt                                                                    >  1:522394/1‑140 (MQ=255)
                                                                                             gcTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgt                                                                    <  2:522394/140‑1 (MQ=255)
                                                                                                   cTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTc                                                    >  2:469754/1‑150 (MQ=255)
                                                                                                            gCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                           >  2:365837/1‑86 (MQ=255)
                                                                                                            gCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATg                                                                                                           <  1:365837/86‑1 (MQ=255)
                                                                                                               gggCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgc                                        <  1:555348/150‑1 (MQ=255)
                                                                                                                   aTAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTg                                    >  2:84584/1‑150 (MQ=255)
                                                                                                                     aGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCa                                  >  1:251219/1‑150 (MQ=255)
                                                                                                                              ccGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACa                         <  1:482584/150‑1 (MQ=255)
                                                                                                                                        ttcttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAg               >  2:25080/1‑150 (MQ=255)
                                                                                                                                          cttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCt                             >  1:357782/1‑134 (MQ=255)
                                                                                                                                          cttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCt                             <  2:357782/134‑1 (MQ=255)
                                                                                                                                          cttcGCGGGCTCGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCt             >  1:528887/1‑150 (MQ=255)
                                                                                                                                           ttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTc            >  1:224933/1‑150 (MQ=255)
                                                                                                                                                     aGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTgc  <  1:469754/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGGCTGGGGGAAAGCCAGTGTATACCTCAGGATGCGCTAGGGGCTGACGAGCATCGGCGCTTGCGGGTGCGGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGC  >  NC_002947/1625101‑1625399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑