| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,629,498 | A→C | 14.0% | *322Y (TAA→TAC) | mucB → | sigma factor AlgU regulator MucB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,629,498 | 0 | A | C | 14.0% | 115.9 / 13.4 | 50 | *322Y (TAA→TAC) | mucB | sigma factor AlgU regulator MucB |
| Reads supporting (aligned to +/- strand): ref base A (20/23); new base C (3/4); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
ACCCAGCTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGG > NC_002947/1629349‑1629637 | aCCCAGCTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTaa < 2:332860/150‑1 (MQ=255) ccAGCTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATg > 2:247932/1‑150 (MQ=255) gCTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGgcg > 1:336656/1‑150 (MQ=255) cTAGGCCTGCCCTCGGCAGTTTCGCGTCGCCTGAATACACCCACGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACTCTTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCATTACTGgcgc < 2:166731/150‑1 (MQ=255) cTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGgcgc > 2:624379/1‑150 (MQ=255) cTAGGCACGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGgcgc < 1:36357/150‑1 (MQ=255) ccGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtttt > 2:81533/1‑150 (MQ=255) ccGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtttt > 2:195154/1‑150 (MQ=255) ccGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtttt > 1:41865/1‑150 (MQ=255) tCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGc > 2:82006/1‑150 (MQ=255) tCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGGACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGc > 1:743495/1‑150 (MQ=255) ctcGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCaa > 2:361191/1‑150 (MQ=255) tcGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAg > 2:686089/1‑150 (MQ=255) cgcgTCGCCTGAATACACCCAGGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAgg < 1:361191/150‑1 (MQ=255) cgcgTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTATGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAgg < 1:106306/150‑1 (MQ=255) gcgTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGc > 2:7386/1‑150 (MQ=255) gTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAg < 2:336656/150‑1 (MQ=255) gTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAg < 2:470580/150‑1 (MQ=255) gCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTg < 2:533822/150‑1 (MQ=255) ccTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTgg < 1:7386/150‑1 (MQ=255) tGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGc < 2:41865/150‑1 (MQ=255) aaTACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCAt > 2:21811/1‑150 (MQ=255) cacCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGc < 1:21811/150‑1 (MQ=255) ccAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGACCGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCtg > 1:105819/1‑150 (MQ=255) ccAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCATTACTGgcgcgcct > 2:167192/1‑112 (MQ=255) ccAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCATTACTGgcgcgcct < 1:167192/114‑3 (MQ=255) aaGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaa < 2:630555/150‑1 (MQ=255) cAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaaatgaa < 1:82006/150‑1 (MQ=255) cAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaaatgaa < 1:195154/150‑1 (MQ=255) ggTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCa > 1:712633/1‑150 (MQ=255) gTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTg < 1:81533/150‑1 (MQ=255) aTTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATc < 1:379918/150‑1 (MQ=255) aTTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATc > 1:657875/1‑150 (MQ=255) aTTCCGCTGGGTACGGCAGAACGCGTCGCCCTGGCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTTGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATc < 1:603809/150‑1 (MQ=255) gggTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAAtt > 2:148734/1‑150 (MQ=255) ggCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCta > 1:384435/1‑149 (MQ=255) ggCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCta < 2:384435/149‑1 (MQ=255) cAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAg < 1:247932/150‑1 (MQ=255) cgTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGc < 1:624379/150‑1 (MQ=255) cGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCttt < 1:148734/150‑1 (MQ=255) cccTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGa > 2:504774/1‑150 (MQ=255) ccTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCAGCGGCAAATTTTCTATAGGTCAGGCTTTGaa > 1:329312/1‑150 (MQ=255) ggATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGc < 1:686089/150‑1 (MQ=255) gcaaaagcgcgcCATTACTGGCGCGCCTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCtttt > 2:286965/7‑142 (MQ=255) gcaaaagcgcgcCATTACTGGCGCGCCTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCtttt < 1:286965/136‑1 (MQ=255) gCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTg < 2:6232/135‑1 (MQ=255) gCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTg > 1:6232/1‑135 (MQ=255) ggCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAg < 2:743495/150‑1 (MQ=255) agcgcgcCATTACTGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCt > 2:639312/2‑129 (MQ=255) agcgcgcCATTACTGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCt < 1:639312/128‑1 (MQ=255) cgcgcCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAggg < 2:657875/150‑1 (MQ=255) | ACCCAGCTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGG > NC_002947/1629349‑1629637 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |