| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,632,892 | T→A | 10.0% | I535N (ATT→AAT) | lepA → | elongation factor 4 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,632,892 | 0 | T | A | 10.0% | 107.1 / 3.0 | 42 | I535N (ATT→AAT) | lepA | elongation factor 4 |
| Reads supporting (aligned to +/- strand): ref base T (16/20); new base A (2/2); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGAAACGCATGAAACAGGTGGGCAATGTG > NC_002947/1632753‑1633040 | cTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGCGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAg < 1:385169/150‑1 (MQ=255) gATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCCGCCAATGGCTGcctgga < 1:390013/150‑5 (MQ=255) tCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCg > 2:453012/1‑150 (MQ=255) ccTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGgc < 2:168384/150‑1 (MQ=255) aTTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCag > 1:186569/1‑150 (MQ=255) aTTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCag > 2:749283/1‑150 (MQ=255) gATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCCGCCAATGGCTGcct > 1:281116/1‑113 (MQ=255) gATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCCGCCAATGGCTGcct < 2:281116/114‑2 (MQ=255) gTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCCCCAACAGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAAc > 1:480368/1‑150 (MQ=255) aCCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGATCAGAAAGAACGTAc > 1:731803/1‑150 (MQ=255) ccGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACt > 1:34714/1‑150 (MQ=255) ccGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACt < 2:186569/150‑1 (MQ=255) cgcgACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTg < 2:34714/150‑1 (MQ=255) aacgcacgGCCTTTATAGGCC‑‑GCGTCGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGGCGTAGCGATCCAGGCAGCCAATGGCGGCCAGATCATTGGGGGGACAACCGTCAAGGCGCCCAGAAAGAACGTCCTGGCCAAg > 2:411075/7‑150 (MQ=255) aaCGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAg > 2:181022/1‑150 (MQ=255) aCGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGACGAAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGt < 1:649498/150‑1 (MQ=255) cGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGCTCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGcc < 1:295466/150‑1 (MQ=255) cGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGt > 2:238331/1‑138 (MQ=255) cGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGt < 1:238331/138‑1 (MQ=255) gaTGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAgct > 2:481359/1‑150 (MQ=255) tGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCTATCCAGGCAGCCATTggc < 2:737450/56‑1 (MQ=255) tGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTggc > 1:737450/1‑56 (MQ=255) tGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAcctgc < 2:260453/150‑1 (MQ=255) aaGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCAGTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGctgctg > 2:32837/1‑150 (MQ=255) cttaccccTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCAATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTAAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAg < 2:731803/145‑1 (MQ=255) cTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAg < 1:749283/150‑1 (MQ=255) gCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTa < 2:798350/85‑1 (MQ=255) gCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTa > 1:798350/1‑85 (MQ=255) gCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCgg < 1:481359/150‑1 (MQ=255) ttCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACACCCGTCAAGGCGCACAGCACGCACGTACAGGCCAACTGCTACGCCGGTGACGACAGCCCCAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGaaa > 2:312787/1‑150 (MQ=255) gACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGAAACGc < 1:453012/150‑1 (MQ=255) aCGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGAAACGCa > 2:768805/1‑150 (MQ=255) gTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTaagaa > 2:27860/1‑110 (MQ=255) gTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTaagaa < 1:27860/110‑1 (MQ=255) aTCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAgg < 2:243870/49‑1 (MQ=255) aTCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAgg > 1:243870/1‑49 (MQ=255) ccAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGAAACGCATGAAACAGGt > 1:523656/1‑150 (MQ=255) cAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGAAACGCATGAAACAGGTg < 2:549304/150‑1 (MQ=255) aGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTATGGAGGTGACGTCATACGGAAGGATAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGAAACGCATGAAACAGGTgg < 1:531711/150‑1 (MQ=255) aGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTTCTGGAGAAGCAGAAGGCCGGTAAGAAACGCATGAAACAGGTgg < 1:181022/150‑1 (MQ=255) aGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGAAACGCATGAAACAGGTgg > 2:374667/1‑150 (MQ=255) aTTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGAAACGCATGAAACAGGTGGGCAAtgtg < 1:768805/150‑1 (MQ=255) | CTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGGTGACGTCAGCCGTAAGAAGAAGCTGCTGGAGAAGCAGAAGGCCGGTAAGAAACGCATGAAACAGGTGGGCAATGTG > NC_002947/1632753‑1633040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |