| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,666,823 | A→G | 10.2% | intergenic (‑53/‑218) | PP_1460 ← / → ffh | membrane protein/signal recognition particle GTPase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,666,823 | 0 | A | G | 10.2% | 105.3 / 2.8 | 39 | intergenic (‑53/‑218) | PP_1460/ffh | membrane protein/signal recognition particle GTPase subunit |
| Reads supporting (aligned to +/- strand): ref base A (19/16); new base G (2/2); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
TATAGGCCGGCTGCGATCAGGTTGGGGATGAGGCTGGGTGAGGAGAACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCTGCACTTCGCTATAATCGCCGCCTTGCTG > NC_002947/1666679‑1666969 | tataGGCCGGCTGCGATCAGGTTGGGGATGAGGCTGGGTGAGGAGAACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCC > 2:114705‑M1/1‑150 (MQ=255) gCCGGCTGCGATCAGGTTGGGGATGAGGCTGGGTGAGGAGAACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCC > 2:481963‑M1/1‑150 (MQ=255) cTGCGATCAGGTTGGGGATGAGGCTGGGTGAGGAGAACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAG < 1:114705‑M1/150‑1 (MQ=255) aTCAGGTTGGGGATGAGGCTGGGTGAGGAGAACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGatcc > 2:565592‑M1/1‑146 (MQ=255) aTGAGGCTGGGTGAGGAGAACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCGCGCCCGCTCCCACAGGatcctcactacctgca > 1:621383‑M1/1‑134 (MQ=255) gTGAGGAGAACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCA > 2:436958‑M1/1‑120 (MQ=255) gTGAGGAGAACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCA < 1:436958‑M1/120‑1 (MQ=255) gagaACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCTTAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATcc < 1:412269‑M2/150‑3 (MQ=255) aCATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATTAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCGCGCCCGCTCCCACAGGatcctcactacctgcaggcctgcgccatacctgtg < 2:573091‑M1/150‑36 (MQ=255) cATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGatcctcactacctgcaggcctgcgccatacctgtgg < 1:565592‑M1/150‑37 (MQ=255) aaGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATg < 2:625988/115‑1 (MQ=255) aaGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATg > 1:625988/1‑115 (MQ=255) aGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCGCGCCCGCTCCCACAGGatcctcactacctgcaggcctgcgccatacctgtgggagc < 2:621383‑M1/150‑41 (MQ=255) ggTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGgcgc > 2:127140/1‑150 (MQ=255) ttGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGgcgcgc < 1:481963/150‑1 (MQ=255) gAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCcgcg > 2:586482/1‑150 (MQ=255) aaGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCa < 1:127140/150‑1 (MQ=255) gATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTa > 2:270582/1‑150 (MQ=255) cGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTa > 1:73873/1‑145 (MQ=255) cGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTa < 2:73873/145‑1 (MQ=255) gCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTAc > 1:56291/1‑145 (MQ=255) gCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTAc < 2:56291/145‑1 (MQ=255) gCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACA < 1:514713‑M1/60‑1 (MQ=255) gCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACA > 2:514713‑M1/1‑60 (MQ=255) aGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGcgtc > 2:286316/1‑150 (MQ=255) ggAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGTTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATc < 2:374708/133‑1 (MQ=255) ggAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATc > 1:374708/1‑133 (MQ=255) gAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGatcctcactacctgcaggcctgcgccatacctgtgggagcgg < 2:816156‑M1/94‑43 (MQ=255) gAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGatcctcactacctgcaggcctgcgccatacctgtgggagcgg > 1:816156‑M1/1‑52 (MQ=255) aCTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCt < 1:286316/150‑1 (MQ=255) tGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCTGc > 2:465379/1‑150 (MQ=255) aaGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCTGCAc > 1:682550/1‑150 (MQ=255) aaGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCTGCAc > 1:170856/1‑150 (MQ=255) gCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCTGCACTTc > 2:767396/1‑150 (MQ=255) aTTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCTGCACTTCGCTATAATcgccgc < 2:682550/150‑1 (MQ=255) cGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCTGCACTTCGCTATAATCGCCGCCtt < 2:170856/150‑1 (MQ=255) ggCGCGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCTGCACTTCGCTATAATCGCCGCCTTGCtg > 2:267613/1‑150 (MQ=255) ggCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAaga < 2:549994/77‑1 (MQ=255) ggCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAaga > 1:549994/1‑77 (MQ=255) | TATAGGCCGGCTGCGATCAGGTTGGGGATGAGGCTGGGTGAGGAGAACATAAGTCCTGGTTGGCGAGCCTTTAAAGAGACGGAGTTTGGCATAGATTCCGCTGATCAAGGAAGACTGAAGGCTTGTGTCGGGGCATTCGTGGGCACGCCCGCTCCCACAGGTATGGCGCAGGCCTGCAGGTAGTGAGGATCCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCAGCACAGCCACCGAATATCTACCAAGGTGTGCGTCGTCGCTGCACTTCGCTATAATCGCCGCCTTGCTG > NC_002947/1666679‑1666969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |