Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,668,384 G→C 7.8% G448G (GGG→GGC)  ffh → signal recognition particle GTPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,668,3840GC 7.8% 157.0 / 5.0 52G448G (GGG→GGC) ffhsignal recognition particle GTPase subunit
Reads supporting (aligned to +/- strand):  ref base G (21/26);  new base C (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GCCGTATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGA  >  NC_002947/1668248‑1668518
                                                                                                                                        |                                                                                                                                      
gcCGTATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGAtgc                                                                                                                                    <  1:539911/141‑1 (MQ=255)
gcCGTATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGAtgc                                                                                                                                    >  2:539911/1‑141 (MQ=255)
 ccGTATTGTGTTGGGTTGCGGTACCCGGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTGTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTgg                                                                                                                          <  2:130851/150‑1 (MQ=255)
    tATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTggcgg                                                                                                                       <  1:378079/150‑1 (MQ=255)
     aTTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTggcggc                                                                                                                      >  2:770898/1‑150 (MQ=255)
         cGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGc                                                                                                                  <  2:665298/150‑1 (MQ=255)
             gggTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtc                                                                                                                   >  1:201829/1‑144 (MQ=255)
             gggTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtc                                                                                                                   <  2:201829/145‑2 (MQ=255)
               gTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAGGGCGCCAGGGCCAAAATGATGCGCGGCCCCGGGGGGATGCTGCCAGGTGGGGGCATGCCAACGc                                                                                                            >  1:556609/1‑150 (MQ=255)
               gTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGCTGCGCGGCCTCGGTGGTATGCTGCCAGGTGGCGGCATGCCAAAGc                                                                                                            >  2:733738/1‑150 (MQ=255)
                     gTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAc                                                                                                      <  1:541427/150‑1 (MQ=255)
                         ccAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATg                                                                                                                   >  2:59121/1‑133 (MQ=255)
                         ccAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATg                                                                                                                   <  1:59121/133‑1 (MQ=255)
                           aGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGa                                                                                                <  1:770898/150‑1 (MQ=255)
                                 aGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCggg                                                                                          <  2:581533/150‑1 (MQ=255)
                                 aGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAACATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCggg                                                                                          >  2:142456/1‑150 (MQ=255)
                                  ggACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGt                                                                                         <  2:482560/150‑1 (MQ=255)
                                   gACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAccc                                                                                                    <  2:395948/138‑1 (MQ=255)
                                   gACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAccc                                                                                                    >  1:395948/1‑138 (MQ=255)
                                             ggcTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCtttt                                                                              >  2:239214/1‑150 (MQ=255)
                                                      agcagcACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTg                                                                                                 <  2:367773/122‑1 (MQ=255)
                                                      agcagcACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTg                                                                                                 >  1:367773/1‑122 (MQ=255)
                                                          gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTaa                                                                                                       >  2:356006/1‑112 (MQ=255)
                                                          gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTaa                                                                                                       <  1:356006/112‑1 (MQ=255)
                                                          gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGgcgc                                                                 >  2:378125/1‑150 (MQ=255)
                                                          gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGgcgc                                                                 >  1:631948/1‑150 (MQ=255)
                                                            acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTa                                                                                                        >  2:327633/1‑109 (MQ=255)
                                                            acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTa                                                                                                        <  1:327633/109‑1 (MQ=255)
                                                            acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGccc                                                               >  2:254362/1‑150 (MQ=255)
                                                            acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtcc                                                                                                                  <  1:208710/99‑3 (MQ=38)
                                                            acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtcc                                                                                                                  >  2:208710/1‑97 (MQ=38)
                                                              aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTgcctgcct                                                                                 >  1:106462/1‑130 (MQ=255)
                                                              aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTgcctgcct                                                                                 <  2:106462/130‑1 (MQ=255)
                                                              aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCtttt                                                                              <  2:389282/133‑1 (MQ=255)
                                                              aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCtttt                                                                              >  1:389282/1‑133 (MQ=255)
                                                              aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAg                                                             >  1:462874/1‑150 (MQ=255)
                                                                         gaagaTGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTg                                                  <  1:103150/150‑1 (MQ=255)
                                                                         gaagaTGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTg                                                  >  2:595373/1‑150 (MQ=255)
                                                                           agaTGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTa                                                >  1:370317/1‑150 (MQ=255)
                                                                            gatgatGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAcccc                                                                                                   >  1:9248/1‑98 (MQ=255)
                                                                            gatgatGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAcccc                                                                                                   <  2:9248/98‑1 (MQ=255)
                                                                                 tgaAGACGTTTTCCGCCAAAGGGGACGGGTTTAAGTGGATGCTCGGCCTCGGTGGGATGCTGCCAGTTGGCTTCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTACAGCgggg                                          <  1:232816/150‑1 (MQ=255)
                                                                                              tGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGaa                             <  1:239214/150‑1 (MQ=255)
                                                                                               gCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAAt                            >  2:536005/1‑150 (MQ=255)
                                                                                                ccAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAAcccc                                                       >  2:6891/1‑122 (MQ=255)
                                                                                                ccAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAAcccc                                                       <  1:6891/122‑1 (MQ=255)
                                                                                                     ggcggcATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTcgcg                      <  2:556609/150‑1 (MQ=255)
                                                                                                       cggcATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCgcggc                    <  1:378125/150‑1 (MQ=255)
                                                                                                        ggcATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAAATTCgcggct                   <  2:631948/150‑2 (MQ=255)
                                                                                                            tGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAAc               <  2:370317/150‑1 (MQ=255)
                                                                                                                       atgCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTg    <  1:254362/150‑1 (MQ=255)
                                                                                                                         gcgcGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGa  <  1:142456/150‑1 (MQ=255)
                                                                                                                                        gATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCgg            <  2:654641/125‑1 (MQ=255)
                                                                                                                                        gATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCgg            >  1:654641/1‑125 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GCCGTATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGA  >  NC_002947/1668248‑1668518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑