Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,668,389 T→C 7.7% L450P (CTG→CCG)  ffh → signal recognition particle GTPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,668,3890TC 7.7% 148.9 / 2.1 52L450P (CTG→CCG) ffhsignal recognition particle GTPase subunit
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base C (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CCGTATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGA  >  NC_002947/1668249‑1668518
                                                                                                                                            |                                                                                                                                 
ccGTATTGTGTTGGGTTGCGGTACCCGGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTGTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTgg                                                                                                                          <  2:130851/150‑1 (MQ=255)
   tATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTggcgg                                                                                                                       <  1:378079/150‑1 (MQ=255)
    aTTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTggcggc                                                                                                                      >  2:770898/1‑150 (MQ=255)
        cGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGc                                                                                                                  <  2:665298/150‑1 (MQ=255)
            gggTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtc                                                                                                                   >  1:201829/1‑144 (MQ=255)
            gggTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtc                                                                                                                   <  2:201829/145‑2 (MQ=255)
              gTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAGGGCGCCAGGGCCAAAATGATGCGCGGCCCCGGGGGGATGCTGCCAGGTGGGGGCATGCCAACGc                                                                                                            >  1:556609/1‑150 (MQ=255)
              gTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGCTGCGCGGCCTCGGTGGTATGCTGCCAGGTGGCGGCATGCCAAAGc                                                                                                            >  2:733738/1‑150 (MQ=255)
                    gTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAc                                                                                                      <  1:541427/150‑1 (MQ=255)
                        ccAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATg                                                                                                                   >  2:59121/1‑133 (MQ=255)
                        ccAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATg                                                                                                                   <  1:59121/133‑1 (MQ=255)
                          aGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGa                                                                                                <  1:770898/150‑1 (MQ=255)
                                aGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCggg                                                                                          <  2:581533/150‑1 (MQ=255)
                                aGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAACATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCggg                                                                                          >  2:142456/1‑150 (MQ=255)
                                 ggACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGt                                                                                         <  2:482560/150‑1 (MQ=255)
                                  gACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAccc                                                                                                    >  1:395948/1‑138 (MQ=255)
                                  gACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAccc                                                                                                    <  2:395948/138‑1 (MQ=255)
                                            ggcTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCtttt                                                                              >  2:239214/1‑150 (MQ=255)
                                                     agcagcACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTg                                                                                                 <  2:367773/122‑1 (MQ=255)
                                                     agcagcACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTg                                                                                                 >  1:367773/1‑122 (MQ=255)
                                                         gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTaa                                                                                                       >  2:356006/1‑112 (MQ=255)
                                                         gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTaa                                                                                                       <  1:356006/112‑1 (MQ=255)
                                                         gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGgcgc                                                                 >  2:378125/1‑150 (MQ=255)
                                                         gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGgcgc                                                                 >  1:631948/1‑150 (MQ=255)
                                                           acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTa                                                                                                        >  2:327633/1‑109 (MQ=255)
                                                           acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTa                                                                                                        <  1:327633/109‑1 (MQ=255)
                                                           acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGccc                                                               >  2:254362/1‑150 (MQ=255)
                                                           acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtcc                                                                                                                  <  1:208710/99‑3 (MQ=38)
                                                           acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtcc                                                                                                                  >  2:208710/1‑97 (MQ=38)
                                                             aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTgcctgcct                                                                                 >  1:106462/1‑130 (MQ=255)
                                                             aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTgcctgcct                                                                                 <  2:106462/130‑1 (MQ=255)
                                                             aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCtttt                                                                              >  1:389282/1‑133 (MQ=255)
                                                             aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCtttt                                                                              <  2:389282/133‑1 (MQ=255)
                                                             aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAg                                                             >  1:462874/1‑150 (MQ=255)
                                                                        gaagaTGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTg                                                  <  1:103150/150‑1 (MQ=255)
                                                                        gaagaTGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTg                                                  >  2:595373/1‑150 (MQ=255)
                                                                          agaTGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTa                                                >  1:370317/1‑150 (MQ=255)
                                                                           gatgatGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAcccc                                                                                                   >  1:9248/1‑98 (MQ=255)
                                                                           gatgatGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAcccc                                                                                                   <  2:9248/98‑1 (MQ=255)
                                                                                tgaAGACGTTTTCCGCCAAAGGGGACGGGTTTAAGTGGATGCTCGGCCTCGGTGGGATGCTGCCAGTTGGCTTCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTACAGCgggg                                          <  1:232816/150‑1 (MQ=255)
                                                                                             tGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGaa                             <  1:239214/150‑1 (MQ=255)
                                                                                              gCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAAt                            >  2:536005/1‑150 (MQ=255)
                                                                                               ccAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAAcccc                                                       >  2:6891/1‑122 (MQ=255)
                                                                                               ccAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAAcccc                                                       <  1:6891/122‑1 (MQ=255)
                                                                                                    ggcggcATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTcgcg                      <  2:556609/150‑1 (MQ=255)
                                                                                                      cggcATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCgcggc                    <  1:378125/150‑1 (MQ=255)
                                                                                                       ggcATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAAATTCgcggct                   <  2:631948/150‑2 (MQ=255)
                                                                                                           tGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAAc               <  2:370317/150‑1 (MQ=255)
                                                                                                                      atgCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTg    <  1:254362/150‑1 (MQ=255)
                                                                                                                        gcgcGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGa  <  1:142456/150‑1 (MQ=255)
                                                                                                                                       gATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCgg            <  2:654641/125‑1 (MQ=255)
                                                                                                                                       gATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCgg            >  1:654641/1‑125 (MQ=255)
                                                                                                                                            |                                                                                                                                 
CCGTATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGA  >  NC_002947/1668249‑1668518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑