Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,668,400 G→A 8.0% G454S (GGC→AGC)  ffh → signal recognition particle GTPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,668,4000GA 8.0% 139.6 / 2.2 50G454S (GGC→AGC) ffhsignal recognition particle GTPase subunit
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base A (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGACGGCAGGGCGAGGCCTTGCCGAAAA  >  NC_002947/1668252‑1668543
                                                                                                                                                    |                                                                                                                                               
tATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTggcgg                                                                                                                                                <  1:378079/150‑1 (MQ=255)
 aTTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTggcggc                                                                                                                                               >  2:770898/1‑150 (MQ=255)
     cGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGc                                                                                                                                           <  2:665298/150‑1 (MQ=255)
         gggTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtc                                                                                                                                            >  1:201829/1‑144 (MQ=255)
         gggTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtc                                                                                                                                            <  2:201829/145‑2 (MQ=255)
           gTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAGGGCGCCAGGGCCAAAATGATGCGCGGCCCCGGGGGGATGCTGCCAGGTGGGGGCATGCCAACGc                                                                                                                                     >  1:556609/1‑150 (MQ=255)
           gTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGCTGCGCGGCCTCGGTGGTATGCTGCCAGGTGGCGGCATGCCAAAGc                                                                                                                                     >  2:733738/1‑150 (MQ=255)
                 gTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAc                                                                                                                               <  1:541427/150‑1 (MQ=255)
                     ccAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATg                                                                                                                                            >  2:59121/1‑133 (MQ=255)
                     ccAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATg                                                                                                                                            <  1:59121/133‑1 (MQ=255)
                       aGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGa                                                                                                                         <  1:770898/150‑1 (MQ=255)
                             aGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCggg                                                                                                                   <  2:581533/150‑1 (MQ=255)
                             aGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAACATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCggg                                                                                                                   >  2:142456/1‑150 (MQ=255)
                              ggACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGt                                                                                                                  <  2:482560/150‑1 (MQ=255)
                               gACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAccc                                                                                                                             >  1:395948/1‑138 (MQ=255)
                               gACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAccc                                                                                                                             <  2:395948/138‑1 (MQ=255)
                                         ggcTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCtttt                                                                                                       >  2:239214/1‑150 (MQ=255)
                                                  agcagcACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTg                                                                                                                          <  2:367773/122‑1 (MQ=255)
                                                  agcagcACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTg                                                                                                                          >  1:367773/1‑122 (MQ=255)
                                                      gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTaa                                                                                                                                <  1:356006/112‑1 (MQ=255)
                                                      gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTaa                                                                                                                                >  2:356006/1‑112 (MQ=255)
                                                      gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGgcgc                                                                                          >  2:378125/1‑150 (MQ=255)
                                                      gcaCAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGgcgc                                                                                          >  1:631948/1‑150 (MQ=255)
                                                        acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTa                                                                                                                                 >  2:327633/1‑109 (MQ=255)
                                                        acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTa                                                                                                                                 <  1:327633/109‑1 (MQ=255)
                                                        acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGccc                                                                                        >  2:254362/1‑150 (MQ=255)
                                                        acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtcc                                                                                                                                           <  1:208710/99‑3 (MQ=38)
                                                        acaAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGCATGCCGCCACCTGGCAGCAtcc                                                                                                                                           >  2:208710/1‑97 (MQ=38)
                                                          aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTgcctgcct                                                                                                          >  1:106462/1‑130 (MQ=255)
                                                          aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTgcctgcct                                                                                                          <  2:106462/130‑1 (MQ=255)
                                                          aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCtttt                                                                                                       >  1:389282/1‑133 (MQ=255)
                                                          aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCtttt                                                                                                       <  2:389282/133‑1 (MQ=255)
                                                          aaGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAg                                                                                      >  1:462874/1‑150 (MQ=255)
                                                                     gaagaTGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTg                                                                           <  1:103150/150‑1 (MQ=255)
                                                                     gaagaTGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTg                                                                           >  2:595373/1‑150 (MQ=255)
                                                                       agaTGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTa                                                                         >  1:370317/1‑150 (MQ=255)
                                                                        gatgatGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAcccc                                                                                                                            >  1:9248/1‑98 (MQ=255)
                                                                        gatgatGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAAcccc                                                                                                                            <  2:9248/98‑1 (MQ=255)
                                                                             tgaAGACGTTTTCCGCCAAAGGGGACGGGTTTAAGTGGATGCTCGGCCTCGGTGGGATGCTGCCAGTTGGCTTCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTACAGCgggg                                                                   <  1:232816/150‑1 (MQ=255)
                                                                                          tGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGaa                                                      <  1:239214/150‑1 (MQ=255)
                                                                                           gCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAAt                                                     >  2:536005/1‑150 (MQ=255)
                                                                                            ccAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAAcccc                                                                                >  2:6891/1‑122 (MQ=255)
                                                                                            ccAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAAcccc                                                                                <  1:6891/122‑1 (MQ=255)
                                                                                                 ggcggcATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTcgcg                                               <  2:556609/150‑1 (MQ=255)
                                                                                                   cggcATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCgcggc                                             <  1:378125/150‑1 (MQ=255)
                                                                                                    ggcATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAAATTCgcggct                                            <  2:631948/150‑2 (MQ=255)
                                                                                                        tGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAAc                                        <  2:370317/150‑1 (MQ=255)
                                                                                                                   atgCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTg                             <  1:254362/150‑1 (MQ=255)
                                                                                                                     gcgcGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGa                           <  1:142456/150‑1 (MQ=255)
                                                                                                                                    gATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCgg                                     <  2:654641/125‑1 (MQ=255)
                                                                                                                                    gATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCgg                                     >  1:654641/1‑125 (MQ=255)
                                                                                                                                              ggTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGACGGCAGGGCGAGGCCTTGCCGaaaa  >  2:374172/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TATTGCGTTGGGTTCCGGTACCCAGGTGCAGGACATCGGCCGGCTGATCAAGCAGCACAAGCAGATGCAGAAGATGATGAAGAAGTTTTCTGCCAAAGGCGGCATGGCCAAGATGATGCGCGGCCTCGGTGGGATGCTGCCAGGTGGCGGCATGCCAAAGCTGTAACCCCTGATCCGGGTGCCTGCCTTTTGTCGGGCAGGCGCCCAGAACCCCCGCTGTAGCGGGGCAACAGCCGCGAATTTCGCGGCGCAACCGGCAAATCTGGACGGCAGGGCGAGGCCTTGCCGAAAA  >  NC_002947/1668252‑1668543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑