| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,703,290 | A→G | 9.4% | intergenic (+97/‑70) | lysS → / → PP_1497 | lysine‑‑tRNA ligase/TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,703,290 | 0 | A | G | 9.4% | 136.5 / 2.0 | 51 | intergenic (+97/‑70) | lysS/PP_1497 | lysine‑‑tRNA ligase/TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (23/23); new base G (2/3); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAC > NC_002947/1703141‑1703428 | cGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACaaa < 1:420083/150‑1 (MQ=255) gCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAg > 1:43449/1‑150 (MQ=255) ccGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAgg < 2:43449/150‑1 (MQ=255) gATCCGCGAGGTCATCCTGTTCGCGCACATGCGTCCGGAGGCGTGAGTGAGCTGAACGAGCCGCGTTTGGAGGCGGCTGTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTg < 2:130302/150‑1 (MQ=38) tCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCC‑tttgta > 2:6084/1‑146 (MQ=255) cgcgACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGCCT‑TTGTAgg < 2:762591/150‑1 (MQ=255) gACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGc < 2:679171/150‑1 (MQ=255) cGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTACAAGGCCT‑TTGTAGGAGCgg < 2:306415/150‑1 (MQ=255) gTCATCCTGTTCCCGCACATGCGTCCGTAGGCCTGAGTGAGCTGAACGAGCTGTCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCggg < 1:64621/150‑1 (MQ=255) gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCggg > 2:691051/1‑150 (MQ=255) gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCggg < 1:691051/150‑1 (MQ=255) gTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCTTTGTAGGAGCggg < 1:553942/150‑1 (MQ=255) tCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGt > 1:105613/1‑150 (MQ=255) tCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGt > 1:547382/1‑150 (MQ=255) aTCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGttt < 2:149233/150‑1 (MQ=255) ccTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCTTTGTAGGAGCGGGTTTAc < 2:556459/150‑1 (MQ=255) cTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCTTTGTAGGAGCgg < 1:131789/143‑1 (MQ=38) cTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCCCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCTTTGTAGGAGCgg > 2:131789/1‑143 (MQ=38) caTGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGc < 1:6084/150‑1 (MQ=255) tGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCa < 2:105613/150‑1 (MQ=255) cAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGGGTTATGTCGGCAGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGGTTACCCGCGAAGGGGCCAGCACGGAc > 2:207792/1‑150 (MQ=37) gCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCggg < 2:329349/120‑1 (MQ=255) gCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCggg > 1:329349/1‑120 (MQ=255) ccTGAGTTAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGaaa < 2:766949/150‑1 (MQ=255) gcggcTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCg > 1:330917/1‑150 (MQ=255) cggcTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGAC‑TCATAGGGTCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGc > 2:669716/1‑150 (MQ=38) tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATg > 2:486352/1‑150 (MQ=255) ttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCt < 2:547382/150‑1 (MQ=255) ttCTTGCCTGAAGCTTTATGGTGTCAGTACCGCCCTTTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCTGGTTTACCCCCGAAGGCGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCt < 2:311640/150‑1 (MQ=38) gAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAg > 1:100478/1‑114 (MQ=255) gAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAg < 2:100478/114‑1 (MQ=255) tgttgtCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAg > 2:129984/1‑55 (MQ=255) tgttgtCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAg < 1:129984/55‑1 (MQ=255) tCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGc > 1:764828/1‑150 (MQ=255) cGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCAccgcc > 2:356538/1‑150 (MQ=255) gCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGt > 1:477893/1‑150 (MQ=255) ttCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGGCACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCg > 1:511214/1‑150 (MQ=255) gcgGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGaaa < 2:477893/150‑1 (MQ=255) gggTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGc < 1:356538/150‑1 (MQ=255) gTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGt < 2:764828/150‑1 (MQ=255) tAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTg > 1:418850/1‑150 (MQ=255) aaCCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGGCCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGcc > 1:477155/1‑149 (MQ=255) aaCCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCa < 2:330917/150‑1 (MQ=255) ccGCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGc > 2:31877/1‑98 (MQ=25) ccGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATa > 1:796502/1‑150 (MQ=255) ccGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCATGACCAAAGCGCAGCAGGCACCGGCACCGCCGGCGCCGAAAGCGTGCAATa > 2:224720/1‑150 (MQ=39) ccGCTCCTAC‑AAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGc < 1:31877/98‑1 (MQ=25) cGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAc > 2:491515/1‑150 (MQ=255) cTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCAccgcc > 2:427981/1‑127 (MQ=255) cTCCTAC‑AAGGCCTTTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCAccgcc < 1:427981/127‑1 (MQ=255) aCAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGc < 1:179122/90‑1 (MQ=255) aCAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGc > 2:179122/1‑90 (MQ=255) | CGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGAGTGAGCTGAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAC > NC_002947/1703141‑1703428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |