Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,807,232 C→G 8.4% I291M (ATC→ATG)  pyrG → CTP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,807,2320CG 8.4% 142.4 / 5.2 48I291M (ATC→ATG) pyrGCTP synthase
Reads supporting (aligned to +/- strand):  ref base C (21/23);  new base G (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

CATCTACAAGATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCT  >  NC_002947/1807085‑1807378
                                                                                                                                                   |                                                                                                                                                  
cATCTACAAGATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGc                                                                                                                                                  >  1:185209/1‑150 (MQ=255)
         gATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATg                                                                                                                                              >  2:472633/1‑145 (MQ=255)
         gATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATg                                                                                                                                              <  1:472633/145‑1 (MQ=255)
          aTCCCGGGCGTGCTGCATCCTCAGGTCCTGGACGACTTCGTCGTCGAGCGCTTCTGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGATCACGAAGTGTCCATCGCCATGGTCAGc                                                                                                                                        >  2:348625/1‑150 (MQ=255)
                ggCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTAc                                                                                                                                  <  1:187772/150‑1 (MQ=255)
                  cGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACAt                                                                                                                                <  2:257630/150‑1 (MQ=255)
                  cGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACAt                                                                                                                                <  1:313621/150‑1 (MQ=255)
                  cGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACAt                                                                                                                                <  2:6531/150‑1 (MQ=255)
                   gTGCTGCATGTTCAGGGCCTTGACGACTTCTTCGTCGATCTCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATg                                                                                                                               <  2:185209/150‑1 (MQ=255)
                     tctgcATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGa                                                                                                                             <  1:345624/149‑1 (MQ=255)
                              tCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATGGCGATGGTcac                                                                                                                                         <  2:236015/129‑3 (MQ=255)
                              tCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATGGCGATGGTcac                                                                                                                                         >  1:236015/1‑127 (MQ=255)
                              tCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATGGCGATGGTcac                                                                                                                                         <  2:659399/129‑3 (MQ=255)
                              tCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATGGCGATGGTcac                                                                                                                                         >  1:659399/1‑127 (MQ=255)
                                 gggCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGc                                                                                                                 <  2:843992/150‑1 (MQ=255)
                                   gCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGCGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGt                                                                                                               >  2:500214/1‑150 (MQ=255)
                                    catGGACGACTTCGTCGTCGACAGCTTAGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGTAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTa                                                                                                              <  2:817631/148‑1 (MQ=255)
                                            acTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGc                                                                                                      <  1:357685/150‑1 (MQ=255)
                                                         gcgcTTCGGCCTGCAGTGCAATGGCGCCGACCTGTGCGAGTTGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGAt                                                                                         <  1:646100/150‑1 (MQ=255)
                                                         gcgcTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGAt                                                                                         >  1:849571/1‑150 (MQ=255)
                                                         gcgcTTCGGCCTGCACTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACCAAGTGACCATCGCCATGGCCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGAt                                                                                         <  1:646318/150‑1 (MQ=255)
                                                            cTTAGGCCTGCATTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAg                                                                                      >  2:33189/1‑150 (MQ=255)
                                                             ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCaa                                                                                                                                      >  1:187595/1‑101 (MQ=255)
                                                             ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCaa                                                                                                                                      <  2:187595/101‑1 (MQ=255)
                                                             ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGc                                                                                     <  1:37959/150‑1 (MQ=255)
                                                             ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGc                                                                                     >  2:434498/1‑150 (MQ=255)
                                                                    tGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGAGCGCGGGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGTGCTGCTGGATGCGTACAAGTCGCTGAGCGAAGCGATGAGCCACGCCg                                                                              >  2:547072/1‑150 (MQ=255)
                                                                     gCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCgg                                                                             >  2:241941/1‑150 (MQ=255)
                                                                                 cgcCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCg                                                                 <  1:33189/150‑1 (MQ=255)
                                                                                       ccTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCaa                                                           >  2:499785/1‑150 (MQ=255)
                                                                                        cTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAg                                                          >  1:35626/1‑150 (MQ=255)
                                                                                             cGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGa                                                                                                                    <  2:455097/87‑1 (MQ=255)
                                                                                             cGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGa                                                                                                                    >  1:455097/1‑87 (MQ=255)
                                                                                               aGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAAcc                                                   >  1:216268/1‑150 (MQ=255)
                                                                                                     aCAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCa                                                                                                                                                <  2:178348/51‑1 (MQ=255)
                                                                                                     aCAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCa                                                                                                                                                >  1:178348/1‑51 (MQ=255)
                                                                                                         ggtggtCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACa                                          <  2:195426/149‑1 (MQ=255)
                                                                                                         ggtggtCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACa                                          >  1:195426/1‑149 (MQ=255)
                                                                                                                         cTCAACCCGGAGCACGAAGTGATCATCGCCATGGTCGGCAAGTACAGGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATc                         <  1:348625/150‑1 (MQ=255)
                                                                                                                            aaCCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCgag                      <  2:216268/150‑1 (MQ=255)
                                                                                                                                      aCGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCa            >  2:507042/1‑150 (MQ=255)
                                                                                                                                        gAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCAcc          >  1:126002/1‑150 (MQ=255)
                                                                                                                                            tGACCTTCGCGCTTGTCGGCGAGTTCATCGAGCTGGTGGATGCGTACAAGTCGCTGATTGATACGATGAGCCGCGCCGGCATCACCCACCGGACCCAGGCCAACCTGAGCTACATGCATTCGGAAGGCGCCGAGCACCGGGGCAGAAGcc      >  2:64397/1‑150 (MQ=255)
                                                                                                                                                cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCAc                                                                       <  1:742677/81‑1 (MQ=255)
                                                                                                                                                cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCAc                                                                       <  1:742382/81‑1 (MQ=255)
                                                                                                                                                cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCAc                                                                       >  2:742382/1‑81 (MQ=255)
                                                                                                                                                cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCAc                                                                       >  2:742677/1‑81 (MQ=255)
                                                                                                                                                cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCt  <  1:500214/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATCTACAAGATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCT  >  NC_002947/1807085‑1807378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑