Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,807,235 C→G 10.0% A292A (GCC→GCG)  pyrG → CTP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,807,2350CG10.0% 137.1 / 5.1 47A292A (GCC→GCG) pyrGCTP synthase
Reads supporting (aligned to +/- strand):  ref base C (19/23);  new base G (3/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCTCGAAG  >  NC_002947/1807094‑1807383
                                                                                                                                             |                                                                                                                                                    
gATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATg                                                                                                                                                   <  1:472633/145‑1 (MQ=255)
gATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATg                                                                                                                                                   >  2:472633/1‑145 (MQ=255)
 aTCCCGGGCGTGCTGCATCCTCAGGTCCTGGACGACTTCGTCGTCGAGCGCTTCTGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGATCACGAAGTGTCCATCGCCATGGTCAGc                                                                                                                                             >  2:348625/1‑150 (MQ=255)
       ggCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTAc                                                                                                                                       <  1:187772/150‑1 (MQ=255)
         cGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACAt                                                                                                                                     <  2:6531/150‑1 (MQ=255)
         cGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACAt                                                                                                                                     <  2:257630/150‑1 (MQ=255)
         cGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACAt                                                                                                                                     <  1:313621/150‑1 (MQ=255)
          gTGCTGCATGTTCAGGGCCTTGACGACTTCTTCGTCGATCTCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATg                                                                                                                                    <  2:185209/150‑1 (MQ=255)
            tctgcATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGa                                                                                                                                  <  1:345624/149‑1 (MQ=255)
                     tCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATGGCGATGGTcac                                                                                                                                              >  1:659399/1‑127 (MQ=255)
                     tCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATGGCGATGGTcac                                                                                                                                              <  2:659399/129‑3 (MQ=255)
                     tCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATGGCGATGGTcac                                                                                                                                              <  2:236015/129‑3 (MQ=255)
                     tCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATGGCGATGGTcac                                                                                                                                              >  1:236015/1‑127 (MQ=255)
                        gggCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGc                                                                                                                      <  2:843992/150‑1 (MQ=255)
                          gCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGCGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGt                                                                                                                    >  2:500214/1‑150 (MQ=255)
                           catGGACGACTTCGTCGTCGACAGCTTAGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGTAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTa                                                                                                                   <  2:817631/148‑1 (MQ=255)
                                   acTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGc                                                                                                           <  1:357685/150‑1 (MQ=255)
                                                gcgcTTCGGCCTGCAGTGCAATGGCGCCGACCTGTGCGAGTTGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGAt                                                                                              <  1:646100/150‑1 (MQ=255)
                                                gcgcTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGAt                                                                                              >  1:849571/1‑150 (MQ=255)
                                                gcgcTTCGGCCTGCACTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACCAAGTGACCATCGCCATGGCCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGAt                                                                                              <  1:646318/150‑1 (MQ=255)
                                                   cTTAGGCCTGCATTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAg                                                                                           >  2:33189/1‑150 (MQ=255)
                                                    ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCaa                                                                                                                                           <  2:187595/101‑1 (MQ=255)
                                                    ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCaa                                                                                                                                           >  1:187595/1‑101 (MQ=255)
                                                    ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGc                                                                                          <  1:37959/150‑1 (MQ=255)
                                                    ttCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGc                                                                                          >  2:434498/1‑150 (MQ=255)
                                                           tGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGAGCGCGGGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGTGCTGCTGGATGCGTACAAGTCGCTGAGCGAAGCGATGAGCCACGCCg                                                                                   >  2:547072/1‑150 (MQ=255)
                                                            gCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCgg                                                                                  >  2:241941/1‑150 (MQ=255)
                                                                        cgcCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCg                                                                      <  1:33189/150‑1 (MQ=255)
                                                                              ccTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCaa                                                                >  2:499785/1‑150 (MQ=255)
                                                                               cTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAg                                                               >  1:35626/1‑150 (MQ=255)
                                                                                    cGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGa                                                                                                                         >  1:455097/1‑87 (MQ=255)
                                                                                    cGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGa                                                                                                                         <  2:455097/87‑1 (MQ=255)
                                                                                      aGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAAcc                                                        >  1:216268/1‑150 (MQ=255)
                                                                                            aCAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCa                                                                                                                                                     >  1:178348/1‑51 (MQ=255)
                                                                                            aCAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCa                                                                                                                                                     <  2:178348/51‑1 (MQ=255)
                                                                                                ggtggtCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACa                                               <  2:195426/149‑1 (MQ=255)
                                                                                                ggtggtCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACa                                               >  1:195426/1‑149 (MQ=255)
                                                                                                                cTCAACCCGGAGCACGAAGTGATCATCGCCATGGTCGGCAAGTACAGGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATc                              <  1:348625/150‑1 (MQ=255)
                                                                                                                   aaCCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCgag                           <  2:216268/150‑1 (MQ=255)
                                                                                                                             aCGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCa                 >  2:507042/1‑150 (MQ=255)
                                                                                                                               gAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCAcc               >  1:126002/1‑150 (MQ=255)
                                                                                                                                   tGACCTTCGCGCTTGTCGGCGAGTTCATCGAGCTGGTGGATGCGTACAAGTCGCTGATTGATACGATGAGCCGCGCCGGCATCACCCACCGGACCCAGGCCAACCTGAGCTACATGCATTCGGAAGGCGCCGAGCACCGGGGCAGAAGcc           >  2:64397/1‑150 (MQ=255)
                                                                                                                                       cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCAc                                                                            <  1:742677/81‑1 (MQ=255)
                                                                                                                                       cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCAc                                                                            <  1:742382/81‑1 (MQ=255)
                                                                                                                                       cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCAc                                                                            >  2:742382/1‑81 (MQ=255)
                                                                                                                                       cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCAc                                                                            >  2:742677/1‑81 (MQ=255)
                                                                                                                                       cATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCt       <  1:500214/150‑1 (MQ=255)
                                                                                                                                            ccATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCTCGAAg  <  2:849571/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GATCCCGGGCGTGCTGCATGCTCAGGGCCTGGACGACTTCGTCGTCGAGCGCTTCGGCCTGCAGTGCAATGGCGCCGACCTGTCCGAGTGGGACAAGGTGGTCGATGCCAAGCTCAACCCGGAGCACGAAGTGACCATCGCCATGGTCGGCAAGTACATGGAGCTGCTGGATGCGTACAAGTCGCTGATCGAAGCGATGAGCCACGCCGGCATCACCAACCGGACCAAGGTCAACCTGCGCTACATCGATTCGGAAGACATCGAGAACCAGGGCACCAGCCTGCTCGAAG  >  NC_002947/1807094‑1807383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑