Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,899,850 A→T 16.7% K120M (AAG→ATG) ‡ PP_1703 → assimilatory nitrate reductase/sulfite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,899,8500AT16.7% 88.6 / 11.1 36K120M (AAG→ATG) ‡PP_1703assimilatory nitrate reductase/sulfite reductase
Reads supporting (aligned to +/- strand):  ref base A (11/19);  new base T (3/3);  total (14/22)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CCAGCCGCCAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTATTTCTG  >  NC_002947/1899704‑1899998
                                                                                                                                                  |                                                                                                                                                    
ccagccGCCAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGtt                                                                                                                                                   <  1:301432/150‑1 (MQ=255)
    ccgccAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGcc                                                                                                                                               <  2:657788/150‑1 (MQ=255)
      gccAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCaa                                                                                                                                             >  1:642684/1‑150 (MQ=255)
        cAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAgg                                                                                                                                           <  2:176891/150‑1 (MQ=255)
        cAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAgg                                                                                                                                           <  1:495247/150‑1 (MQ=255)
              gcACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTa                                                                                                                                     <  1:556329/150‑1 (MQ=255)
               cACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAATTGTCGCTGGAGGCCCTTTACCTGTCCAACAAGTTTGCCAAGGGCTTTAt                                                                                                                                    <  2:737503/150‑1 (MQ=255)
                 ccAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAAGGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATcc                                                                                                                                  <  2:846673/150‑1 (MQ=255)
                              ggCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTCCGCGCCACAt                                                                                                                     >  2:136738/1‑150 (MQ=255)
                                  aTCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAg                                                                                                                 <  2:642684/150‑1 (MQ=255)
                                       tGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCaa                                                                                                            <  2:68383/150‑1 (MQ=255)
                                         ccACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACTTGTTGGCCa                                                                                                                                              >  1:24833/1‑114 (MQ=255)
                                         ccACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACTTGTTGGCCa                                                                                                                                              <  2:24833/114‑1 (MQ=255)
                                           aCCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATcc                                                                                                                                  <  1:345297/124‑1 (MQ=255)
                                           aCCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATcc                                                                                                                                  >  2:345297/1‑124 (MQ=255)
                                              gccgAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCATCTCgcgc                                                                                                     <  1:355358/150‑1 (MQ=255)
                                                           gcgGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgcca                                                                                        <  1:354048/150‑1 (MQ=255)
                                                            cgGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgc                                                                                          >  2:113686/1‑147 (MQ=255)
                                                            cgGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGgc                                                                                          <  1:113686/147‑1 (MQ=255)
                                                                           gCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTa                                                                        <  1:202823/150‑1 (MQ=255)
                                                                                 cccGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCt                                                                  <  2:415393/150‑1 (MQ=255)
                                                                                                       gtgtCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGCTGGCCCa                                            >  1:84270/1‑150 (MQ=255)
                                                                                                          tCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCt                                         >  2:150563/1‑150 (MQ=255)
                                                                                                            cGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTgg                                       >  2:189915/1‑150 (MQ=255)
                                                                                                             gggCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGa                                                                                                                  <  2:775179/74‑1 (MQ=255)
                                                                                                             gggCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGa                                                                                                                  >  1:775179/1‑74 (MQ=255)
                                                                                                                        gCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGa                           >  1:607993/1‑150 (MQ=255)
                                                                                                                                        ttGGCCAACTTGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCtgt                                                                                                 >  1:781718/2‑64 (MQ=38)
                                                                                                                                        ttGGCCAACTTGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCtgt                                                                                                 <  2:781718/63‑1 (MQ=38)
                                                                                                                                        ttGGCCAACTTGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCa                                     >  2:333495/2‑124 (MQ=255)
                                                                                                                                        ttGGCCAACTTGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCa                                     <  1:333495/123‑1 (MQ=255)
                                                                                                                                        cTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCtt                                                           >  1:218301/1‑102 (MQ=255)
                                                                                                                                        cTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCtt                                                           <  2:218301/102‑1 (MQ=255)
                                                                                                                                            ccAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGt                                                                <  1:749762/93‑1 (MQ=255)
                                                                                                                                            ccAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGt                                                                >  2:749762/1‑93 (MQ=255)
                                                                                                                                            ccAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTAt       <  2:764201/150‑1 (MQ=255)
                                                                                                                                              aacaaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCa                                     <  1:84516/118‑1 (MQ=255)
                                                                                                                                              aacaaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCa                                     >  2:84516/1‑118 (MQ=255)
                                                                                                                                                 aaGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCCCCAGCAGTGGCTACAAGCTGTCGCTTTGCTCCGATGGCCCACCTGGCATTTACCAGGACTTCGAGCACGCCGACGTATTTCTg  <  1:154313/150‑1 (MQ=255)
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CCAGCCGCCAGTGCGCACCAGCATGGACCAGGCCATCGCTGCCACCGCCGAGCGTCTGCGCGGCATCATCGACCAGCACGGCCCGGATGCCGTGGCGCTGTACGTGTCCGGGCAAATGTCGCTGGAGGCCCAGTACCTGGCCAACAAGTTGGCCAAGGGCTTTATCCGTACGCGCCACATCGAGTCCAACTCGCGCCTGTGCATGGCCAGCGCCAGCAGTGGCTACAAGCTGTCGCTTGGCGCCGATGGCCCACCTGGCAGTTACCAGGACTTCGAGCACGCCGACGTATTTCTG  >  NC_002947/1899704‑1899998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑