Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,077,807 C→T 14.0% A163V (GCG→GTG)  PP_1853 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,077,8070CT14.0% 102.8 / 7.1 42A163V (GCG→GTG) PP_1853LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (23/13);  new base T (3/3);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTTGCGCCGATACCCAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGTTGG  >  NC_002947/2077665‑2077952
                                                                                                                                              |                                                                                                                                                 
ttCTTGCGCCGATACCCAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCgg                                                                                                                                            <  2:271507/150‑1 (MQ=255)
       gcCGATACCCAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGTGCGCCggggtcagc                                                                                                                                     <  1:376900/150‑7 (MQ=255)
               ccAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGgcggcg                                                                                                                             >  2:201619/1‑150 (MQ=255)
                cAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGgcgc                                                                                                                            >  2:106746/1‑150 (MQ=255)
                           ccGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTg                                                                                                                 >  2:67756/1‑150 (MQ=255)
                                   gCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCa                                                                                                         >  1:577816/1‑150 (MQ=255)
                                      tttCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCAcgc                                                                                                      >  1:744928/1‑150 (MQ=255)
                                          gACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCg                                                                                                  >  1:440004/1‑150 (MQ=255)
                                             aaCCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGTGCGCCggggtca                                                                                                                                       >  1:368690/1‑106 (MQ=255)
                                             aaCCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGTGCGCCggggtca                                                                                                                                       <  2:368690/110‑5 (MQ=255)
                                                 gCCAGGTCGACTTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCt                                                                                                                      >  2:514500/1‑123 (MQ=255)
                                                 gCCAGGTCGACTTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCt                                                                                                                      <  1:514500/123‑1 (MQ=255)
                                                      gTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCg                                                                                      <  1:201619/150‑1 (MQ=255)
                                                              gATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCt                                                                              <  2:328509/150‑1 (MQ=255)
                                                              gATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCATATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCt                                                                              >  1:512091/1‑150 (MQ=255)
                                                                   gCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTtggtgg                                                                                                                                  >  2:600512/1‑93 (MQ=255)
                                                                   gCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTtggtgg                                                                                                                                  <  1:600512/93‑1 (MQ=255)
                                                                    ccAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTtggt                                                                                                                                    >  1:827451/1‑90 (MQ=255)
                                                                    ccAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTtggt                                                                                                                                    <  2:827451/90‑1 (MQ=255)
                                                                           ttCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGCTAGAAGACCGCCAGGCGCTGCAACGGCATGaa                                                                 >  1:402978/1‑149 (MQ=255)
                                                                           ttCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGAc                                                                 <  1:515393/150‑1 (MQ=255)
                                                                           ttCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGAc                                                                 >  2:515393/1‑150 (MQ=255)
                                                                                   atcGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCt                                                         >  1:762778/3‑150 (MQ=255)
                                                                                   aGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCt                                                         >  2:326857/1‑150 (MQ=255)
                                                                                      gATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGt                                                      >  1:125928/1‑150 (MQ=255)
                                                                                      gATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGt                                                      <  2:744928/150‑1 (MQ=255)
                                                                                       aTTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTg                                                     <  2:762778/150‑1 (MQ=255)
                                                                                           gCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGt                                                 >  2:446934/1‑150 (MQ=255)
                                                                                                   ttCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAg                                         >  2:405690/1‑150 (MQ=255)
                                                                                                       aaCTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACcg                                     >  1:421185/1‑150 (MQ=255)
                                                                                                              gccgGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGt                              <  1:446934/150‑1 (MQ=255)
                                                                                                                      tggtggCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGt                      <  1:609895/150‑1 (MQ=255)
                                                                                                                        gtggCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCa                                                                     >  1:702564/1‑101 (MQ=255)
                                                                                                                        gtggCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCa                                                                     <  2:702564/101‑1 (MQ=255)
                                                                                                                             gcgcAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGtt               <  2:440004/150‑1 (MQ=255)
                                                                                                                                     aacacccGGTGCGCCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGcc       >  2:24185/5‑150 (MQ=255)
                                                                                                                                     aacacccGGTGCGCCGGGTGATGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGCCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTAAGCAGcc       >  2:832529/5‑150 (MQ=255)
                                                                                                                                       aCCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGt     >  1:458935/1‑150 (MQ=255)
                                                                                                                                         cccGGTGCGCCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGTTg   <  1:24185/150‑1 (MQ=255)
                                                                                                                                         cccGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGTTg   >  1:609086/1‑150 (MQ=255)
                                                                                                                                          ccGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGTTgg  >  1:561833/1‑150 (MQ=255)
                                                                                                                                          ccGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGTTgg  >  1:17672/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
TTCTTGCGCCGATACCCAGCGATTACCCCGGATTGGCATTTCGACAACCGCCAGGTCGACCTGATCGGCCAGGGCTTCGATGCAGCGATTGGCGGTGGTTTCGAACTGCCGCCGGGGGTGGTGGCGCGCAAGCTGACCCCGGCGCACCGGGTGTTGGTGGCGGCGCCGGCCTATCTGCAGCGGCACGCGTCGATAGAAGACCCGCAGGCGCTGCAACGCCATGACGGCATCCTGGTGCGGTCACCGCAGACCGGGCGGGTGCGCTCGTGGCCGTTGACCAGCCGTTGG  >  NC_002947/2077665‑2077952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑