| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,128,306 | C→A | 12.4% | L1212M (CTG→ATG) | PP_1887 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,128,306 | 0 | C | A | 12.4% | 107.7 / 4.3 | 40 | L1212M (CTG→ATG) | PP_1887 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (19/16); new base A (3/2); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGTATGAC > NC_002947/2128161‑2128446 | cAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGga < 2:618699/150‑1 (MQ=255) ggCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGgaga < 2:322209/150‑1 (MQ=255) cTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTg > 1:282518/1‑150 (MQ=255) caacaTCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTggagga < 1:438920/150‑1 (MQ=255) aTCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATGTGGCACAAATGAGCCGGATCATTGCGCCTGCTGCGCTACTGGAGACCTATGACGTCGATAACGACCTGGAGGAgttg > 2:308506/1‑150 (MQ=255) aTCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAgttg > 2:640887/1‑150 (MQ=255) tcgatcGGTGATCAACGACAGACCGGTGGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAgttgtt < 2:689502/146‑1 (MQ=255) ccTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAgttgt < 2:635059/149‑1 (MQ=255) ccTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAggtgt > 1:635059/1‑149 (MQ=255) tctcGGTGATCACCTACAGACCGGACGGGTCGTGGCACCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGCGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCa > 1:689502/1‑150 (MQ=255) gTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATc < 2:282518/150‑1 (MQ=255) tGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCg > 2:764940/1‑150 (MQ=255) aCCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGAc < 1:219748/111‑1 (MQ=255) aCCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGAc > 2:219748/1‑111 (MQ=255) aGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGAt > 2:790190/1‑150 (MQ=255) aGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTgga > 2:310436/1‑103 (MQ=255) aGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTgga < 1:310436/103‑1 (MQ=255) tGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTAATGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTacg > 2:37281/1‑150 (MQ=255) tGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTAATGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTacg > 2:37406/1‑150 (MQ=255) tACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACATGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTacgac > 2:754824/1‑150 (MQ=255) gAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGc > 2:414729/1‑150 (MQ=255) gCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGa < 1:640887/150‑1 (MQ=255) cGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGAt < 1:60812/150‑1 (MQ=255) aTCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTAATGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCa < 1:37406/150‑1 (MQ=255) aTCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTAATGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCa < 1:37281/150‑1 (MQ=255) cGGCACAAATGAGCCGGATCATTCCGCCTGATGCACTAAAGGCGACCTCTTCATACCAGTACGACCTGGAGGATTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGtg > 1:16108/1‑150 (MQ=255) ggCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATc < 1:284548/98‑1 (MQ=255) ggCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATc > 2:284548/1‑98 (MQ=255) ggCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGtga > 1:355713/1‑150 (MQ=255) gCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCaa > 2:826770/1‑137 (MQ=255) gCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCaa < 1:826770/137‑1 (MQ=255) aTGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAg < 1:764940/150‑1 (MQ=255) tGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATTACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAgg < 1:308506/150‑1 (MQ=255) ccGGATCATTCCGCCTGATGCGCTACTGGAGAGCTATGACGTCAATAGCGCCCTTTGGGCGAGGTGCAGGTTTGTCGAATACGATCCGCAGGGGGGTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCg > 2:737250/1‑150 (MQ=255) ccGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCg > 2:695524/1‑150 (MQ=255) cATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACt > 2:414740/1‑150 (MQ=255) cGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGt > 1:49171/1‑150 (MQ=255) ccTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCg > 2:386281/1‑75 (MQ=255) ccTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCg < 1:386281/75‑1 (MQ=255) gATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGTATGAc < 1:414740/150‑1 (MQ=255) | CAGGCAGATATTCGGCTTCGATGGCCTGGACAACATCCTCTCGGTGATCACCTACAGACCGGACGGGTCGTGGCAGCGGACCTTGTACGAATTCAAGAATAGCGATCCGGCACAAATGAGCCGGATCATTCCGCCTGATGCGCTACTGGAGACCTATGACGTCGATCACGACCTGGAGGAGTTGTTCAAGATCGTCAAATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGTATGAC > NC_002947/2128161‑2128446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |