Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,185,374 C→A 17.2% A760S (GCA→TCA)  PP_1936 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,185,3740CA17.2% 49.4 / 3.2 24A760S (GCA→TCA) PP_1936membrane protein
Reads supporting (aligned to +/- strand):  ref base C (11/8);  new base A (2/2);  total (13/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

TTTCATGAGTAAGTAAGAGTCCAGTAGTGTCAAAGTTTTCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGTTGTCCTTTACGATCAGCCGAACATTGGAGTCGTAGAAGCATTAGATCATTCCAACCCAGCGCACCTC  >  NC_002947/2185227‑2185517
                                                                                                                                                   |                                                                                                                                               
tttCATGAGTAAGTAAGAGTCCAGTAGTGTCGAAGTTTTCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCa                                                                                                                                               >  2:604930/1‑150 (MQ=255)
              aagAGTCCAGTAGTGTCAAAGTTTTCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGACAGTCTGCTta                                                                                                                                      >  2:512466/1‑145 (MQ=255)
              aagAGTCCAGTAGTGTCAAAGTTTTCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGACAGTCTGCTta                                                                                                                                      >  2:512071/1‑145 (MQ=255)
              aagAGTCCAGTAGTGTCAAAGTTTTCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGACAGTCTGCTta                                                                                                                                      <  1:512466/145‑1 (MQ=255)
              aagAGTCCAGTAGTGTCAAAGTTTTCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGACAGTCTGCTta                                                                                                                                      <  1:512071/145‑1 (MQ=255)
                   tCCAGTAGTGTCAAAGTTTTCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGa                                                                                                                            <  1:739147/150‑1 (MQ=255)
                           tgtCAAAGTTTTCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTTCTCGGCTTTGAATTTGGCAAAGACTATTTGGAAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTtgctg                                                                                                                    >  2:164259/1‑150 (MQ=255)
                                     ttCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTg                                                                                                          <  1:604930/150‑1 (MQ=255)
                                                  gcgTCTACAGTGTCATAAAAGTTGATTTTTATATGGCGCGCCTCTTTTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCAGGTGATCGACCACAATg                                                                                             >  2:590582/1‑150 (MQ=255)
                                                        aCAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGcc                                                                                       >  1:455920/1‑150 (MQ=255)
                                                                ctAAAAGTTGATTTTTTTGTCTTTGTGCTGGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAg                                                                               >  2:402416/2‑150 (MQ=255)
                                                                 tAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCgg                                                                                                             >  2:194802/1‑119 (MQ=255)
                                                                 tAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCgg                                                                                                             <  1:194802/119‑1 (MQ=255)
                                                                        tGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCGAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTaaa                                                                       >  1:370073/1‑150 (MQ=255)
                                                                           tttttttGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGtt                                                                    >  1:250716/1‑150 (MQ=255)
                                                                                ttGTCAATGTGCTCGGCTTTGAATTTGGCGAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGTTGTCCt                                                               <  2:370073/150‑1 (MQ=255)
                                                                                               gCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGTTGTCCTTTACGATCAGCCGaa                                                <  2:455920/150‑1 (MQ=255)
                                                                                                         tGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGTTGTCCTTTACGATCAGCCGAACATTGGAGTc                                      <  1:34427/150‑1 (MQ=255)
                                                                                                                 aCTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGTTGTCCTTTACGATCAGCCGAACATTGGAGTCGTAGAAGc                              >  2:349554/1‑150 (MQ=255)
                                                                                                                                   ctcATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTTCATTCGGGTGATCGTCCACAATGAGCGCCTTTTGAAGATGGTAAAGTTGTCTTTTACGATCAGCCGAACATTGGAGTCGTAGAAGCATTAGATCATTccctgac            <  1:590582/150‑6 (MQ=255)
                                                                                                                                    tcATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGTTGTCCTTTACGATCAGCCGAAc                                               <  2:131733/114‑1 (MQ=255)
                                                                                                                                    tcATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGTTGTCCTTTACGATCAGCCGAAc                                               >  1:131733/1‑114 (MQ=255)
                                                                                                                                    tcATGCACATAGTTCTCTGCCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCAAATTCAGCGGTTTTTGAAGATTGTAAAGTTGTCCTTTACGATCAGCCGAACATTGGAGTCGTAGAAGCATTAGATCATTCCAACCCa           <  1:402416/150‑1 (MQ=255)
                                                                                                                                             tAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGTTGTCCTTTACGATCAGCCGAACATTGGAGTCGTAGAAGCATTAGATCATTCCAACCCAGCGCACCTc  >  1:603002/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TTTCATGAGTAAGTAAGAGTCCAGTAGTGTCAAAGTTTTCGTCAGGAATCGCGTCTACAGTGTCATAAAAGTTGATTTTTTTGTCAATGTGCTCGGCTTTGAATTTGGCAAAGACTAGTTGGGAGATTCTTCTCATGCCCATAATTGCCAGTCTGCTTATAACTCCTGAGGTTGCTGCATTCGGGTGATCGACCACAATGAGCGCCTTTTGAAGATTGTAAAGTTGTCCTTTACGATCAGCCGAACATTGGAGTCGTAGAAGCATTAGATCATTCCAACCCAGCGCACCTC  >  NC_002947/2185227‑2185517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑