| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,277,210 | G→C | 11.1% | A52G (GCC→GGC) | PP_2006 ← | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,277,210 | 0 | G | C | 11.1% | 104.8 / 5.6 | 36 | A52G (GCC→GGC) | PP_2006 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (19/13); new base C (2/2); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
CGACCCCGGCGTCGATGTTGGCGGGGTAGGTTTTTTCCAGGGCGATGCGCTGGTTGAGCGCAGAGACCCGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCGGACAGCATGACCTTGCTTTCATT > NC_002947/2277064‑2277358 | cGACCCCGGCGTCGATGTTGGCGGGGTAGGTTTTTTCCAGGGCGATGCGCTGGTTGAGCGCAGAGACCCGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTgcgcggcgc < 1:486437/150‑1 (MQ=255) cGGCGTCGATGTTGGCGGGGTAGGTTTTTTCCAGGGCGATGCGCTGGTTGAGCGCAGAGACCCGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAgg < 1:536907/150‑1 (MQ=255) tCGATGTTGGCGGGGTAGGTTTTTTCCAGGGCGATGCGCTGGTTGAGCGCAGAGACCCGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCt > 2:448690/1‑150 (MQ=255) ggCGGGGTAGGTTTTTTCCAGGGCGATGCGCTGGTTGAGCGCAGAGACCCGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACt < 2:733734/150‑1 (MQ=255) gTTTTTTCCAGGGCGATGCGCTGGTTGAGCGCAGAGACCCGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAAc > 2:661048/1‑150 (MQ=255) aGGGCGATGCGCTGGTTGAGCGCAGAGACCCGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTg > 1:750478/1‑150 (MQ=255) aCCCGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTc > 1:701596/1‑150 (MQ=255) ccGGCAGATGCTCACGGTATCCCAGCGTTCCGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTg > 1:157361/1‑150 (MQ=255) ccGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTg > 2:686504/1‑150 (MQ=255) ccGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTg > 1:410251/1‑150 (MQ=255) cGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTgg > 2:123462/1‑150 (MQ=255) tCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGttt > 2:104361/1‑150 (MQ=255) ggTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCAtt < 1:448690/150‑1 (MQ=255) ggTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCAtt < 2:701596/150‑1 (MQ=255) tATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGTGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAggg > 2:430547/1‑114 (MQ=255) tATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGTGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAggg < 1:430547/114‑1 (MQ=255) tCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAgg > 2:30372/1‑150 (MQ=255) aGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACt > 1:471847/1‑79 (MQ=255) aGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACt < 2:471847/79‑1 (MQ=255) gCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATc < 1:453042/150‑1 (MQ=255) gCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATc < 1:661048/150‑1 (MQ=255) gTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGc < 1:104361/150‑1 (MQ=255) gTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATg < 2:439218/150‑1 (MQ=255) ggCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTg > 2:193273/1‑150 (MQ=255) ccAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTggg > 1:339173/1‑150 (MQ=255) cAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGc > 1:103109/1‑150 (MQ=255) gtTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAAc < 2:189385/150‑1 (MQ=255) ccGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCg < 2:157361/150‑1 (MQ=255) cGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCgg > 2:720700/1‑150 (MQ=255) ggCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCGGa > 1:114952/1‑150 (MQ=255) gTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCGGACAGCATg > 1:90704/1‑150 (MQ=255) gTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCGGACAGc > 2:271707/1‑143 (MQ=255) gTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCGGACAGc < 1:271707/143‑1 (MQ=255) accttGCGCGCCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTggg > 1:63216/5‑128 (MQ=255) accttGCGCGCCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTggg < 2:63216/124‑1 (MQ=255) cttGCGCGCCGCGCAAGGTGTCTTCGGAACTGTGTACAAAc < 1:489574/39‑1 (MQ=255) cttGCGCGCCGCGCAAGGTGTCTTCGGAACTGTGTACAAAc > 2:489574/3‑41 (MQ=255) gcggcgcgCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCGGACAGCATGACCTTGCTTTCa < 1:193273/150‑1 (MQ=255) ggcgcgCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCGGACAGCATGACCTTGCTTTCAtt < 2:103109/150‑1 (MQ=255) | CGACCCCGGCGTCGATGTTGGCGGGGTAGGTTTTTTCCAGGGCGATGCGCTGGTTGAGCGCAGAGACCCGGCAGATGCTCACGGTATCCCAGCCATTGGTCGTGACCGTACTGGCCAGTGTTCCGGCATTGGTCAGTAGCTGCGCGGCGCGCAAGGTGTCTTCGGAACTGTGTACAAACGCCGCGTTGCGGTACAGGGGCGATTCGCGCAGGGTCTGGCGCGCGGTTTCATTGAGGTGATCGAGGCGCAGCGAGATGGGTTGGGCAAACCCGGACAGCATGACCTTGCTTTCATT > NC_002947/2277064‑2277358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |