| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,334,712 | G→A | 16.8% | R596R (CGC→CGT) | PP_2052 ← | bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,334,712 | 0 | G | A | 16.8% | 37.8 / 3.8 | 24 | R596R (CGC→CGT) | PP_2052 | bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (10/10); new base A (2/2); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01 | |||||||||||
TTGCTGTTGCTGCAGTTCAGGGCGTGGTGAATGGCGAGCCCGGCGCCAAACGCCAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTC > NC_002947/2334572‑2334861 | ttgctgttgctgCACATCAGGGCGAGGTGAATGGCGAGCCCGGCGCCCCACGCCAGGGCCGGGGCTGCAATACCGTGCTTGCGGGCAACGTCGATGCGGCTGAGGATGCGTTCGTTGCGCTGCACCTTGCGCCAGGGGTCGCGGCCTAcc > 2:275536/1‑150 (MQ=255) tgttgctgCAGTTCAGGGCGTGGTGAATGGCGAGCCCGGCGCCAAACGCCAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCggg > 1:88010/1‑150 (MQ=255) tgcAGTTCAGGGCGTGGTGAATGGCGAGCCCGGCGCCAAACGCCAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAgg > 2:183415/1‑150 (MQ=255) gcAGTTCAGGGCGTGGTGAATGGCGAGCCCGGCGCCAAACGCCAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAggg > 1:174766/1‑150 (MQ=255) gtggtgAATGGCGAGCCCGGCGCCAAACGCCAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGTGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGa < 1:134250/150‑1 (MQ=255) aaTGGCGAGCCCGGCGCCAAACGCCAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGtttt > 2:68369/1‑150 (MQ=255) ggCGAGCCCGGCGCCAAACGCCAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCTTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCa < 1:349614/150‑1 (MQ=255) gAGCCCGGCGCCAAACGCCAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAgcg < 1:68369/150‑1 (MQ=255) cAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGc > 2:431696/1‑150 (MQ=255) cAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCACGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGc > 1:583945/1‑150 (MQ=255) cAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCACGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGc > 1:583981/1‑150 (MQ=255) gggTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGc < 1:151060/150‑1 (MQ=255) aCCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTc > 1:36282/1‑150 (MQ=255) ttGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATc > 2:528077/1‑150 (MQ=255) cGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCtgt < 1:183415/150‑1 (MQ=255) gAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGc < 2:1587/150‑1 (MQ=255) gAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCACGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGc < 2:583981/150‑1 (MQ=255) gAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCACCGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGc < 2:583945/150‑1 (MQ=255) ggATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCgg < 1:301427/150‑1 (MQ=255) gttcgttGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCttt < 1:679526/58‑1 (MQ=255) gttcgttGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCttt > 2:679526/1‑58 (MQ=255) gcgttgcAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTg < 2:88010/150‑1 (MQ=255) ttGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAgg < 2:597230/150‑1 (MQ=255) gcAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCt > 2:190807/1‑150 (MQ=255) gcgGCCTACCCGGGCGCAGGGGTCTTCGGCCGTGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAGGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTtc > 1:663432/1‑150 (MQ=255) | TTGCTGTTGCTGCAGTTCAGGGCGTGGTGAATGGCGAGCCCGGCGCCAAACGCCAGGGCCGGGGTTGCAATACCGTGCTTGCGGGCAAGGTCGATGCTGCTGAGGATGCGTTCGTTGCGTTGCAGCTTGCGCAAGGGGTCGCGGCCTACCCGGGCGCAGGGGTCTTTGAACGAGGTTTTGCAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTC > NC_002947/2334572‑2334861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |