Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,404,284 G→A 10.7% G290S (GGC→AGC) ‡ PP_2106 → ammonium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,404,2840GA10.7% 138.0 / 4.9 55G290S (GGC→AGC) ‡PP_2106ammonium transporter
Reads supporting (aligned to +/- strand):  ref base G (29/20);  new base A (3/3);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTGGCGTGTTCGGCCAGGTGGCGTT  >  NC_002947/2404135‑2404432
                                                                                                                                                      |                                                                                                                                                    
aCTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑g                                                                                                                                                      >  1:38958/1‑150 (MQ=255)
    gTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCTGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCt                                                                                                                                                  <  1:814097/150‑1 (MQ=255)
    gTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCt                                                                                                                                                  >  1:91955/1‑150 (MQ=255)
        aTGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCCCGCCACC‑GGCCtggtg                                                                                                                                              <  1:156013/150‑1 (MQ=255)
            ccATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCgg                                                                                                                                          >  1:213754/1‑150 (MQ=255)
             cATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGGGGGTGCGCTCGCCACC‑GGCCTGGTGGCggg                                                                                                                                         >  1:71841/1‑150 (MQ=255)
                  tgggtggCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATACCGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑AGGCCGGTGGCga                                                                                                                                          >  1:19607/1‑143 (MQ=255)
                  tgggtggCACCCTCTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑AGGCCGGTGGCga                                                                                                                                          <  2:19607/144‑2 (MQ=255)
                     gtggCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGt                                                                                                                                 >  2:785959/1‑150 (MQ=255)
                        gCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTg                                                                                                                              >  2:459650/1‑150 (MQ=255)
                            ccTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTg                                                                                                                          >  1:834644/1‑150 (MQ=255)
                              tGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGa                                                                                                                        <  2:346593/150‑1 (MQ=255)
                              tGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGa                                                                                                                        <  2:91955/150‑1 (MQ=255)
                               gTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATACCGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCTGGTGGGTGCGCTCGCCGCC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGag                                                                                                                       >  1:476455/1‑149 (MQ=255)
                                tCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCCTAACGGCGCGCTGGGCGGGCTGGTGGCGGTTTGCGCAGGTTCCGATCTGGTGCACACGGTGGGTGCGCTCGCCACA‑GGCCTGGTGGCGGGGGCGCGGTTTGTGTGGAcc                                                                                                                      >  2:241094/1‑150 (MQ=255)
                                  aGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCtt                                                                                                                    <  2:38958/150‑1 (MQ=255)
                                    cGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCt                                                                                                                           >  2:785434/1‑141 (MQ=255)
                                    cGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCt                                                                                                                           <  1:785434/141‑1 (MQ=255)
                                                 gcgcAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGttt                                                                                                                               <  2:728658/124‑1 (MQ=255)
                                                 gcgcAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGttt                                                                                                                               >  1:728658/1‑124 (MQ=255)
                                                      aCGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCt                                                                                                <  1:824428/150‑1 (MQ=255)
                                                      aCGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCt                                                                                                <  1:672768/150‑1 (MQ=255)
                                                      aCGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCt                                                                                                <  1:459650/150‑1 (MQ=255)
                                                           ccGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGaaa                                                                                           >  1:848722/1‑150 (MQ=255)
                                                                     tGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGacgacg                                                                                 >  1:629297/1‑150 (MQ=255)
                                                                      gCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACCAGGCC‑GGTGGCga                                                                                                                                          >  2:59863/1‑91 (MQ=255)
                                                                      gCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑AGGCCGGTGGCga                                                                                                                                          <  1:59863/92‑2 (MQ=255)
                                                                       cATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTc                                                                               <  1:785959/150‑1 (MQ=255)
                                                                         tAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCt                                                                             >  1:133354/1‑150 (MQ=255)
                                                                           aCGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTgg                                                                           >  1:313107/1‑150 (MQ=255)
                                                                              gCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCg                                                                        >  2:546207/1‑150 (MQ=255)
                                                                                        cGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCCCGCCACCAGGCC‑GGTGGCgagc                                                                                                                                        >  1:50554/1‑73 (MQ=37)
                                                                                        cGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCCCGCCACCAGGCC‑GGTGGCgagc                                                                                                                                        <  2:50554/76‑4 (MQ=37)
                                                                                             tggtggCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATg                                                         <  2:568320/150‑1 (MQ=255)
                                                                                                      tttGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCtgtgtg                                                >  2:459925/1‑150 (MQ=255)
                                                                                                               gTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTggg                                       <  2:834644/150‑1 (MQ=255)
                                                                                                               gTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTggg                                       <  2:758073/150‑1 (MQ=255)
                                                                                                                  ccGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTg                                    <  1:459925/150‑1 (MQ=255)
                                                                                                                    gATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGc                                  >  2:376102/1‑150 (MQ=255)
                                                                                                                      tCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCAt                                >  1:658064/1‑150 (MQ=255)
                                                                                                                       cTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTc                                                      >  1:337581/1‑127 (MQ=255)
                                                                                                                       cTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTc                                                      <  2:337581/127‑1 (MQ=255)
                                                                                                                        tGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCg                              >  1:135962/1‑150 (MQ=255)
                                                                                                                              aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTggg                                                                          >  2:227610/1‑100 (MQ=255)
                                                                                                                              aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTggg                                                                          <  1:227610/100‑1 (MQ=255)
                                                                                                                              aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCtgtgtg                                                <  2:173361/126‑1 (MQ=255)
                                                                                                                              aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCtgtgtg                                                >  1:173361/1‑126 (MQ=255)
                                                                                                                              aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCtgtg                        >  2:199802/1‑150 (MQ=255)
                                                                                                                               cccGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTgg                       >  2:196886/1‑150 (MQ=255)
                                                                                                                                ccGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGTAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTCTGTGGCGTGTGGGGTGGCATCGCCTGTGGc                      <  1:371220/150‑1 (MQ=255)
                                                                                                                                           gcTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTGGCGTGTTCGGCCa           >  1:557652/1‑150 (MQ=255)
                                                                                                                                           ccccGCCACC‑AGGCCGGTGGCGAGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTGGCGTGTTCGGCCa           >  1:153007/4‑150 (MQ=255)
                                                                                                                                             ccGCCACC‑AGGCCGGTGGCGAGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATc                               >  1:822635/2‑128 (MQ=255)
                                                                                                                                             ccGCCACC‑AGGCCGGTGGCGAGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATc                               <  2:822635/127‑1 (MQ=255)
                                                                                                                                                ccACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTATGGCGTGTTCGGCCAGGTgg      >  1:514093/1‑150 (MQ=255)
                                                                                                                                                    c‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCGCCGCCGCGCAAAATCGCTGGGAAATCGACGACGTCCTGGGCGTCTGGCCGTTGCATGGTCTGTGTGGCGGGTGGGGTGCCACGGCGTGTGGCGTGTTGGTCGAGGTGGCGtt  >  2:351066/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
ACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTGGCGTGTTCGGCCAGGTGGCGTT  >  NC_002947/2404135‑2404432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑