Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,404,288 T→C 12.0% L291P (CTG→CCG)  PP_2106 → ammonium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,404,2880TC12.0% 120.9 / 6.9 52L291P (CTG→CCG) PP_2106ammonium transporter
Reads supporting (aligned to +/- strand):  ref base T (26/17);  new base C (3/3);  total (31/21)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

GTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTGGCGTGTTCGGCCAGGTGGCGTT  >  NC_002947/2404139‑2404432
                                                                                                                                                      |                                                                                                                                                
gTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCTGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCt                                                                                                                                                  <  1:814097/150‑1 (MQ=255)
gTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCt                                                                                                                                                  >  1:91955/1‑150 (MQ=255)
    aTGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCCCGCCACC‑GGCCtggtg                                                                                                                                              <  1:156013/150‑1 (MQ=255)
        ccATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCgg                                                                                                                                          >  1:213754/1‑150 (MQ=255)
         cATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGGGGGTGCGCTCGCCACC‑GGCCTGGTGGCggg                                                                                                                                         >  1:71841/1‑150 (MQ=255)
              tgggtggCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATACCGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑AGGCCGGTGGCga                                                                                                                                          >  1:19607/1‑143 (MQ=255)
              tgggtggCACCCTCTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑AGGCCGGTGGCga                                                                                                                                          <  2:19607/144‑2 (MQ=255)
                 gtggCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGt                                                                                                                                 >  2:785959/1‑150 (MQ=255)
                    gCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTg                                                                                                                              >  2:459650/1‑150 (MQ=255)
                        ccTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTg                                                                                                                          >  1:834644/1‑150 (MQ=255)
                          tGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGa                                                                                                                        <  2:346593/150‑1 (MQ=255)
                          tGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGa                                                                                                                        <  2:91955/150‑1 (MQ=255)
                           gTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATACCGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCTGGTGGGTGCGCTCGCCGCC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGag                                                                                                                       >  1:476455/1‑149 (MQ=255)
                            tCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCCTAACGGCGCGCTGGGCGGGCTGGTGGCGGTTTGCGCAGGTTCCGATCTGGTGCACACGGTGGGTGCGCTCGCCACA‑GGCCTGGTGGCGGGGGCGCGGTTTGTGTGGAcc                                                                                                                      >  2:241094/1‑150 (MQ=255)
                              aGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCtt                                                                                                                    <  2:38958/150‑1 (MQ=255)
                                cGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCt                                                                                                                           >  2:785434/1‑141 (MQ=255)
                                cGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCt                                                                                                                           <  1:785434/141‑1 (MQ=255)
                                             gcgcAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGttt                                                                                                                               <  2:728658/124‑1 (MQ=255)
                                             gcgcAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGttt                                                                                                                               >  1:728658/1‑124 (MQ=255)
                                                  aCGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCt                                                                                                <  1:824428/150‑1 (MQ=255)
                                                  aCGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCt                                                                                                <  1:672768/150‑1 (MQ=255)
                                                  aCGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCt                                                                                                <  1:459650/150‑1 (MQ=255)
                                                       ccGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGaaa                                                                                           >  1:848722/1‑150 (MQ=255)
                                                                 tGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGacgacg                                                                                 >  1:629297/1‑150 (MQ=255)
                                                                  gCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACCAGGCC‑GGTGGCga                                                                                                                                          >  2:59863/1‑91 (MQ=255)
                                                                  gCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑AGGCCGGTGGCga                                                                                                                                          <  1:59863/92‑2 (MQ=255)
                                                                   cATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTc                                                                               <  1:785959/150‑1 (MQ=255)
                                                                     tAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCt                                                                             >  1:133354/1‑150 (MQ=255)
                                                                       aCGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTgg                                                                           >  1:313107/1‑150 (MQ=255)
                                                                          gCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCg                                                                        >  2:546207/1‑150 (MQ=255)
                                                                                    cGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCCCGCCACCAGGCC‑GGTGGCgagc                                                                                                                                        >  1:50554/1‑73 (MQ=37)
                                                                                    cGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCCCGCCACCAGGCC‑GGTGGCgagc                                                                                                                                        <  2:50554/76‑4 (MQ=37)
                                                                                         tggtggCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATg                                                         <  2:568320/150‑1 (MQ=255)
                                                                                                  tttGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCtgtgtg                                                >  2:459925/1‑150 (MQ=255)
                                                                                                           gTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTggg                                       <  2:834644/150‑1 (MQ=255)
                                                                                                           gTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTggg                                       <  2:758073/150‑1 (MQ=255)
                                                                                                              ccGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTg                                    <  1:459925/150‑1 (MQ=255)
                                                                                                                gATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGc                                  >  2:376102/1‑150 (MQ=255)
                                                                                                                  tCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCAt                                >  1:658064/1‑150 (MQ=255)
                                                                                                                   cTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTc                                                      >  1:337581/1‑127 (MQ=255)
                                                                                                                   cTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTc                                                      <  2:337581/127‑1 (MQ=255)
                                                                                                                    tGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCg                              >  1:135962/1‑150 (MQ=255)
                                                                                                                          aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTggg                                                                          >  2:227610/1‑100 (MQ=255)
                                                                                                                          aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTggg                                                                          <  1:227610/100‑1 (MQ=255)
                                                                                                                          aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCtgtgtg                                                <  2:173361/126‑1 (MQ=255)
                                                                                                                          aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCtgtgtg                                                >  1:173361/1‑126 (MQ=255)
                                                                                                                          aCCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCtgtg                        >  2:199802/1‑150 (MQ=255)
                                                                                                                           cccGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTgg                       >  2:196886/1‑150 (MQ=255)
                                                                                                                            ccGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGTAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTCTGTGGCGTGTGGGGTGGCATCGCCTGTGGc                      <  1:371220/150‑1 (MQ=255)
                                                                                                                                       gcTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTGGCGTGTTCGGCCa           >  1:557652/1‑150 (MQ=255)
                                                                                                                                       ccccGCCACC‑AGGCCGGTGGCGAGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTGGCGTGTTCGGCCa           >  1:153007/4‑150 (MQ=255)
                                                                                                                                         ccGCCACC‑AGGCCGGTGGCGAGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATc                               >  1:822635/2‑128 (MQ=255)
                                                                                                                                         ccGCCACC‑AGGCCGGTGGCGAGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATc                               <  2:822635/127‑1 (MQ=255)
                                                                                                                                            ccACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTATGGCGTGTTCGGCCAGGTgg      >  1:514093/1‑150 (MQ=255)
                                                                                                                                                c‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCGCCGCCGCGCAAAATCGCTGGGAAATCGACGACGTCCTGGGCGTCTGGCCGTTGCATGGTCTGTGTGGCGGGTGGGGTGCCACGGCGTGTGGCGTGTTGGTCGAGGTGGCGtt  >  2:351066/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
GTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACC‑GGCCTGGTGGCGGGGGCGCTGTTTGTCTGGACCTTCACCGCCGCGCAAAATCGCTGGAAAATCGACGACGTCCTGGGCGTATGGCCGTTGCATGGTCTGTGTGGCGTGTGGGGTGGCATCGCCTGTGGCGTGTTCGGCCAGGTGGCGTT  >  NC_002947/2404139‑2404432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑