| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,412,948 | G→C | 10.7% | intergenic (+169/‑252) | rlmM → / → PP_2114 | 23S rRNA (cytidine(2498)‑2'‑O)‑methyltransferase RlmM/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,412,948 | 0 | G | C | 10.7% | 103.2 / 3.5 | 37 | intergenic (+169/‑252) | rlmM/PP_2114 | 23S rRNA (cytidine(2498)‑2'‑O)‑methyltransferase RlmM/transposase |
| Reads supporting (aligned to +/- strand): ref base G (14/19); new base C (2/2); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCT > NC_002947/2412799‑2413097 | ttctgcGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatgg > 1:268738/1‑150 (MQ=255) gcgtctgcgtctGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatggatgg < 2:749822/150‑1 (MQ=255) ctgcttctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAgc < 2:191284/150‑1 (MQ=255) gcttctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAgcgc < 1:307175/150‑1 (MQ=255) ttttGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatggat < 2:465662/128‑1 (MQ=255) ttttGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatggat > 1:465662/1‑128 (MQ=255) ttttGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTccc < 1:618405/150‑1 (MQ=255) ttGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg < 2:80951/150‑1 (MQ=40) ttGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCaa < 2:268738/150‑1 (MQ=35) cTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGcc > 2:592506/1‑150 (MQ=38) tAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg < 2:54583/137‑1 (MQ=34) tAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg > 1:54583/1‑137 (MQ=34) tAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGa > 1:693617/1‑150 (MQ=40) gggcgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGGCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAg < 1:853007/148‑1 (MQ=255) gcgcgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatggat < 2:644769/108‑1 (MQ=33) gcgcgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatggat > 1:644769/1‑108 (MQ=32) gcgcgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGTAGCGCGTACTCCCGGGAGCAAGcc < 1:120019/141‑1 (MQ=21) gcgcgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGTAGCGCGTACTCCCGGGAGCAAGcc > 2:120019/1‑141 (MQ=21) gATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGaa < 1:208331/150‑1 (MQ=39) tAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAAcc > 2:145968/1‑150 (MQ=39) gTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATCCATGGGGCCtcagc < 2:24040/111‑5 (MQ=255) gTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATCCATGGGGCCtcagc > 1:24040/1‑107 (MQ=255) ttCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAg < 2:181973/141‑1 (MQ=38) ttCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAg > 1:181973/1‑141 (MQ=39) cGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGt > 1:595588/1‑150 (MQ=39) ttGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAggg > 2:373566/1‑150 (MQ=38) cGACTTCAGGAGGCCGGACGCAGGCCTTGCGAAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCA‑GGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCg < 1:636784/150‑1 (MQ=11) aCTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCgg < 2:693617/150‑1 (MQ=38) cTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAg < 1:301862/98‑1 (MQ=38) cTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAg > 2:301862/1‑98 (MQ=39) gAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACtttt > 2:690222/1‑150 (MQ=40) gAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACtttt > 2:689872/1‑150 (MQ=40) ccGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTcgc < 1:145968/149‑1 (MQ=255) gCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGttt < 1:373566/150‑1 (MQ=39) aTGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGa < 2:368296/150‑1 (MQ=39) cccGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAg > 1:403608/1‑150 (MQ=40) aGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAg < 1:592506/150‑1 (MQ=40) ggCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGa > 1:140761/1‑65 (MQ=255) ggCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGa < 2:140761/65‑1 (MQ=255) ggCCCCATCCATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCAGGATGATAGGa < 2:132128/92‑1 (MQ=14) ggCCCCATCCATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCAGGATGATAGGa > 1:132128/1‑92 (MQ=14) ccATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGtttttt < 2:595403/102‑1 (MQ=255) ccATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGtttttt > 1:595403/1‑102 (MQ=255) atggatggGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAggg > 2:679917/1‑145 (MQ=40) atggatggGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAggg < 1:679917/145‑1 (MQ=40) gggatggGGCCTGCAGGGCGTAGTCCCGGGAGCAAGCGCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATcc < 2:3477/149‑1 (MQ=255) gatggGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCt < 1:102087/150‑1 (MQ=255) | TTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCT > NC_002947/2412799‑2413097 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |