Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,438,447 A→G 8.7% K442E (AAG→GAG)  fadB → enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,438,4470AG 8.7% 129.3 / 2.4 46K442E (AAG→GAG) fadBenoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (20/22);  new base G (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CAATGTCGACATCGTCGTCGAGGCTGTGGTCGAGAACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCAAGAAAATGGGCAAGAACCCGA  >  NC_002947/2438299‑2438591
                                                                                                                                                    |                                                                                                                                                
cAATGTCGACATCGTCGTCGAGGCTGTGGTCGAGAACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCaa                                                                                                                                                 >  1:572307/1‑150 (MQ=255)
    gTCGACATCGTCGTCGAGGCTGTGGTCGAGAACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAgcgc                                                                                                                                             >  1:432361/1‑150 (MQ=255)
     tCGACATCGTCGTCGAGGCTGTGGTCGAGAACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGcc                                                                                                                                            <  2:572307/150‑1 (MQ=255)
                 tcgAGGCTGTGGTCGAGAACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTtcg                                                                                                                                >  1:313255/1‑150 (MQ=255)
                             tCGAGAACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGcc                                                                                                                                            <  2:838121/126‑1 (MQ=255)
                             tCGAGAACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGcc                                                                                                                                            >  1:838121/1‑126 (MQ=255)
                             tCGAGAACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCAct                                                                                                                    >  2:331301/1‑150 (MQ=255)
                               gagaACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCActtc                                                                                                                  >  1:684194/1‑150 (MQ=255)
                                       gAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAAccc                                                                                                          >  1:181804/1‑150 (MQ=255)
                                                gcaagcGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTTGAGCGccttg                                                                                                                                         <  2:761906/110‑4 (MQ=255)
                                                gcaagcGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTTGAGCGccttg                                                                                                                                         <  2:761907/110‑4 (MQ=255)
                                                gcaagcGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTTGAGCGccttg                                                                                                                                         >  1:761907/1‑107 (MQ=255)
                                                gcaagcGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTTGAGCGccttg                                                                                                                                         >  1:761906/1‑107 (MQ=255)
                                                gcaagcGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACat                                                                                                 >  1:440702/1‑150 (MQ=255)
                                                    gcGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACatgatg                                                                                             >  1:532941/1‑150 (MQ=255)
                                                           tGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTgg                                                                                      >  1:486055/1‑150 (MQ=255)
                                                               ggAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGa                                                                                  >  2:530123/1‑150 (MQ=255)
                                                                gAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAGACTGGTGCACATGATGCCGCTGGTTGaa                                                                                 <  1:331310/150‑1 (MQ=255)
                                                                      gAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATc                                                                           >  1:642479/1‑150 (MQ=255)
                                                                                 gAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGaa                                                                <  1:547138/150‑1 (MQ=255)
                                                                                    ggACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTc                                                             <  1:331301/150‑1 (MQ=255)
                                                                                             gATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGGGAAGTCCAGTGACGt                                                    >  1:686943/1‑150 (MQ=255)
                                                                                               tCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTgg                                                  <  1:530123/150‑1 (MQ=255)
                                                                                                    gCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTc                                             <  1:88391/150‑1 (MQ=255)
                                                                                                             acCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGca                                                                                                    >  1:801176/1‑86 (MQ=255)
                                                                                                             acCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGca                                                                                                    <  2:801176/86‑1 (MQ=255)
                                                                                                              ccTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTAGGCATGCACTTACTCACTCCTGTGCATATTATGCCGCTGGTTCGTGTGAGCCGTGTCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCg                                   <  2:313255/150‑1 (MQ=255)
                                                                                                               ctctACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGcca                                         >  2:589036/1‑143 (MQ=255)
                                                                                                               ctctACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGcca                                         <  1:589036/143‑1 (MQ=255)
                                                                                                               ctctACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGt                                  >  1:221739/1‑150 (MQ=255)
                                                                                                               ctctACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGACCGccacgtcact                                  <  2:486055/150‑7 (MQ=255)
                                                                                                                  tACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCAGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGc                               <  2:532941/150‑1 (MQ=255)
                                                                                                                       tctcCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACg                          <  1:425584/150‑1 (MQ=255)
                                                                                                                        ctcAATCTACCTCCTTGCCAATGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGc                         <  2:280317/150‑1 (MQ=255)
                                                                                                                           cATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCaa                      >  2:518805/1‑150 (MQ=255)
                                                                                                                           cATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTACTAAAACCCGGTGCACTGCATGCTTGTTGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCaa                      >  1:104708/1‑150 (MQ=255)
                                                                                                                            aTCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCAAg                     >  1:411859/1‑150 (MQ=255)
                                                                                                                               aaCCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAg                                                                                <  2:264975/88‑1 (MQ=255)
                                                                                                                               aaCCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAg                                                                                >  1:264975/1‑88 (MQ=255)
                                                                                                                                 ccTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCAAGAAAAt                <  2:181804/150‑1 (MQ=255)
                                                                                                                                    gctgGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCAAGAAAATggg             <  2:432361/150‑1 (MQ=255)
                                                                                                                                    gctgGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCAAGAAAATggg             <  1:518805/150‑1 (MQ=255)
                                                                                                                                        gCCAAGGCGCTCAAGCGCCCGAAAAACCTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCAAGAAAATGGGCAAg         <  1:357653/150‑1 (MQ=255)
                                                                                                                                          cAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAg                                                                                <  2:590978/77‑1 (MQ=255)
                                                                                                                                          cAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAg                                                                                >  1:590978/1‑77 (MQ=255)
                                                                                                                                            aGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCAAGAAAATGGGCAAGAAcc     <  2:684194/150‑1 (MQ=255)
                                                                                                                                               cgcTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCAAGAAAATGGGCAAGAACCCGa  <  1:137734/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAATGTCGACATCGTCGTCGAGGCTGTGGTCGAGAACCCGAAGGTCAAGCAAGCGGTACTGGCGGAAGTGGAAGGCCAGGTGAAGGACGATGCGATCCTCGCTTCCAACACCTCTACCATCTCCATCAACCTGCTGGCCAAGGCGCTCAAGCGCCCGGAAAACTTCGTCGGCATGCACTTCTTCAACCCGGTGCACATGATGCCGCTGGTTGAAGTGATCCGTGGCGAGAAGTCCAGTGACGTGGCGGTCGCCACCACCGTGGCCTACGCCAAGAAAATGGGCAAGAACCCGA  >  NC_002947/2438299‑2438591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑