Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,442,716 A→C 16.7% K604T (AAG→ACG)  topA → DNA topoisomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,442,7160AC16.7% 77.8 / 11.6 36K604T (AAG→ACG) topADNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base A (19/11);  new base C (4/2);  total (23/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAGGGC  >  NC_002947/2442567‑2442861
                                                                                                                                                     |                                                                                                                                                  
gaACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCACCACGCCCACCATGTCCACCTTTCCGTGCaa                                                                                                                                                    <  2:692347/150‑1 (MQ=255)
gaACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCaa                                                                                                                                                    <  1:179052/150‑1 (MQ=255)
    cTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGaa                                                                                                                                                <  2:54659/150‑1 (MQ=255)
    cTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGaa                                                                                                                                                <  1:335453/150‑1 (MQ=255)
    cTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGaa                                                                                                                                                <  2:227863/150‑1 (MQ=255)
            cgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑Tgg                                                                                                                                          <  1:243697/147‑1 (MQ=255)
            cgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑Tgg                                                                                                                                          >  2:243697/1‑147 (MQ=255)
                gCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCaa                                                                                                                                                    <  2:427453/134‑1 (MQ=255)
                gCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCaa                                                                                                                                                    >  1:427453/1‑134 (MQ=255)
                             gTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCa                                                                                                                      >  2:726702/1‑150 (MQ=255)
                                aCTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCg                                                                                                                   >  2:850979/1‑150 (MQ=255)
                                 cTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGAAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGc                                                                                                                  <  1:402097/150‑1 (MQ=255)
                                    gaagaaCGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTc                                                                                                               >  1:307468/1‑150 (MQ=255)
                                           gTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgt                                                                                                        <  1:301238/150‑1 (MQ=255)
                                                 gacgaGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTc                                                                                                  >  1:112871/1‑150 (MQ=255)
                                                             ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACg                                                                                                           <  2:623041/129‑1 (MQ=255)
                                                             ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACg                                                                                                           >  1:623041/1‑129 (MQ=255)
                                                             ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGccct                                                                                      <  1:850979/150‑1 (MQ=255)
                                                                       gCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGcc                                                                            >  2:743999/1‑150 (MQ=255)
                                                                         aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCTTGCACGGAAtg‑ttg                                                                                                                                          <  1:235459/86‑3 (MQ=255)
                                                                         aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCTTGCACGGAAtg‑ttg                                                                                                                                          >  2:235459/1‑84 (MQ=255)
                                                                         aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAAtg‑tg                                                                                                                                           <  2:470603/85‑1 (MQ=255)
                                                                         aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAAtg‑tg                                                                                                                                           >  1:470603/1‑85 (MQ=255)
                                                                              gCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTgccgcc                                                                     <  2:112871/150‑1 (MQ=255)
                                                                                   aGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGGGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGGAag                                                                >  1:104044/1‑150 (MQ=255)
                                                                                       tGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatga                                                                                                                               >  1:547307/1‑83 (MQ=255)
                                                                                       tGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatga                                                                                                                               <  2:547307/83‑1 (MQ=255)
                                                                                       tGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTACTCTGGCTACAGCCTGCCGCCGAAAGAgcg                                                            >  1:293117/1‑150 (MQ=255)
                                                                                         ccGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATTATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCt                                                          >  1:477238/1‑150 (MQ=255)
                                                                                           gAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGc                                                        >  1:113860/1‑150 (MQ=255)
                                                                                             gTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACAAACATTACGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTACTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCaa                                                      >  2:399187/1‑150 (MQ=255)
                                                                                                cAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGc                                                   >  2:710346/1‑150 (MQ=255)
                                                                                                                  tGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGcc                                 >  1:724964/1‑150 (MQ=255)
                                                                                                                                      accaCATTCCTTGCACGGAATGTTGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCt              >  2:259170/4‑150 (MQ=255)
                                                                                                                                       cAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTga            >  2:111784/1‑150 (MQ=255)
                                                                                                                                        caCATTCCTTGCGCGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAg                                                     <  2:526061/107‑1 (MQ=255)
                                                                                                                                        caCATTCCTTGCGCGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAg                                                     >  1:526061/2‑108 (MQ=255)
                                                                                                                                        caCATTCCTTGCACGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTgac           >  1:217935/2‑150 (MQ=255)
                                                                                                                                                gTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAggg   >  2:211359/1‑150 (MQ=255)
                                                                                                                                                 tGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAGGGc  >  1:451813/1‑150 (MQ=255)
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GAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAGGGC  >  NC_002947/2442567‑2442861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑