| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,529,044 | G→C | 11.1% | intergenic (+128/‑279) | PP_2217 → / → PP_2218 | enoyl‑CoA hydratase/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,529,044 | 0 | G | C | 11.1% | 98.1 / 3.7 | 36 | intergenic (+128/‑279) | PP_2217/PP_2218 | enoyl‑CoA hydratase/transposase |
| Reads supporting (aligned to +/- strand): ref base G (17/15); new base C (2/2); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGA > NC_002947/2528896‑2529189 | gCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGa > 2:554797/1‑150 (MQ=255) ttCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATgg > 2:5437/1‑143 (MQ=255) ttCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATgg < 1:5437/143‑1 (MQ=255) ttCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCg > 1:846639/1‑150 (MQ=255) cAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGCCTTGTGTGCACCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTc > 2:239677/1‑150 (MQ=18) tCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGcc > 1:545603/1‑150 (MQ=255) tCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGcc > 2:217468/1‑150 (MQ=255) aCTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATATTTCAGGCGACGCAGATTTCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGATTTCGGGAATGGATGCCCGTCAAGCGCTGAGGCCCCat > 1:262266/1‑150 (MQ=17) ttCGTTGCCGTTGGCGTTGCGGTGGCTTTTGCTTCTAAGTGGGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatggatgg < 2:262266/150‑1 (MQ=255) cgttgccgttgccgttgcCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGGGCCCCAAGCGCTGAGGCCCCATGGATgggg < 1:239677/150‑1 (MQ=255) cgttgccgttgccgttgcCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATgggg < 2:545603/150‑1 (MQ=255) gttgccgttgccgttgccGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGc > 1:769255/1‑150 (MQ=255) tgccgttgccgttgccgtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCt < 1:554797/150‑1 (MQ=255) gccgttgccgtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAgcgc > 1:563954/1‑150 (MQ=255) ccgttgccgtGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGcgcg > 2:566358/1‑150 (MQ=255) tGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg < 1:566358/150‑1 (MQ=40) tGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg < 2:846639/150‑1 (MQ=40) ttCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAgcgc < 2:496139/101‑1 (MQ=38) ttCTAAGTGCGCGATAGTTCAGGCGACGCAGATGGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAgcgc > 1:496139/1‑101 (MQ=25) tGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGa < 2:563954/150‑1 (MQ=40) gcgATAGTTCAGGCGACGCAGATTGCGATTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATCCATGCCCGTCac > 2:438546/1‑86 (MQ=11) gcgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATCCATGCCCGTCac < 1:438546/87‑2 (MQ=255) gATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATggg > 2:710616/1‑107 (MQ=33) gATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATggg < 1:710616/107‑1 (MQ=32) ttCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCg > 1:835991/1‑150 (MQ=39) aGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCa < 2:769255/150‑1 (MQ=37) gCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCat > 1:283636/1‑78 (MQ=35) gCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCat < 2:283636/78‑1 (MQ=35) gaggCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACt > 1:716309/1‑91 (MQ=34) gaggCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACt < 2:716309/91‑1 (MQ=34) gAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCa > 2:55326/1‑150 (MQ=39) tGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGt < 1:55326/150‑1 (MQ=35) gCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGtt < 1:167004/150‑1 (MQ=35) gagggaggTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACt > 1:686088/1‑150 (MQ=33) gACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTcgc > 2:455463/1‑150 (MQ=32) aCGGGCATCCATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGttttt < 2:714116/132‑1 (MQ=16) aCGGGCATCCATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGttttt > 1:714116/1‑132 (MQ=16) cATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGa < 1:705050/150‑1 (MQ=32) | GCACAGTTCAAGGACCGTTGATCAGACTCGGTTCGTTGCCGTTGCCGTTGCCGTGGCTTTTGCTTCTAAGTGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGACCGTAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGA > NC_002947/2528896‑2529189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |