| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,650,131 | C→A | 19.3% | intergenic (+27/+107) | PP_2320 → / ← oprI | hypothetical protein/major outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,650,131 | 0 | C | A | 19.3% | 70.7 / 8.5 | 31 | intergenic (+27/+107) | PP_2320/oprI | hypothetical protein/major outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (12/13); new base A (2/4); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
ACTGGGACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAG > NC_002947/2649983‑2650270 | aatGGGACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACAGATGGTGACGAGCATCCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGccc < 2:359274/148‑1 (MQ=255) tGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAg < 2:318632/150‑1 (MQ=255) gTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGCGAACGCCGTCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGc < 2:543357/150‑1 (MQ=255) gTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGc < 1:332704/150‑1 (MQ=255) gCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCACTAAGGGCAGGCCt < 2:643871/150‑1 (MQ=255) gTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCACTAAGGGCAGGCCtgg < 1:458759/150‑3 (MQ=255) tatcttggtggCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCtttt < 1:264669/145‑1 (MQ=255) gACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTc < 1:717881/150‑1 (MQ=255) ggtggCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGGAATTTGGGCGGACCCAGGACTGCCCTTAGTGCAGGCCTTTTcagatg > 2:264669/1‑145 (MQ=255) cAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGAcc > 2:123857/1‑150 (MQ=255) ggTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGc > 1:598052/1‑150 (MQ=255) ggTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGc < 2:598052/150‑1 (MQ=255) gTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGcc < 1:123857/150‑1 (MQ=255) gTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGa > 1:148150/1‑150 (MQ=255) aCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACaa < 2:509738/150‑1 (MQ=255) aGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCcacttgtg > 2:151081/1‑145 (MQ=255) gggCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGt < 1:151081/150‑1 (MQ=255) aCCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCg > 2:262149/1‑105 (MQ=255) aCCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCg < 1:262149/105‑1 (MQ=255) ccGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACctgct > 2:12612/1‑90 (MQ=255) ccGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACctgct < 1:12612/90‑1 (MQ=255) cGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTGCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttctt > 1:108457/1‑150 (MQ=255) gAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGt < 2:148150/150‑1 (MQ=255) ccGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGAttatta > 1:79690/1‑150 (MQ=255) gTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGCTCACGAACG‑ACTAAGAAAACCAATCCACTAGTGGGTACGCTTCTCAATAGCGTGGGATGATTATTACTTGCggctg > 1:233015/1‑150 (MQ=255) tAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGt > 1:617092/1‑123 (MQ=255) tAATTTCGGCGTACCCACGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGt < 2:617092/123‑1 (MQ=255) ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg > 2:585316/1‑150 (MQ=255) aaaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCttt < 2:49828/133‑1 (MQ=255) aaaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCttt > 1:49828/3‑135 (MQ=255) aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGc < 2:179779/72‑1 (MQ=37) aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGc > 1:179779/2‑73 (MQ=37) | ACTGGGACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGG‑TTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAG > NC_002947/2649983‑2650270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |