| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,680,476 | A→T | 12.5% | A274A (GCT→GCA) ‡ | PP_2350 ← | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,680,476 | 0 | A | T | 12.5% | 83.0 / 5.0 | 32 | A274A (GCT→GCA) ‡ | PP_2350 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (11/17); new base T (2/2); total (13/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
GGTGGGGGTGATCACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATA > NC_002947/2680361‑2680610 | ggtggGGGTGATCACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGtt < 1:349647/150‑1 (MQ=255) ggggTGATCACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAg < 2:827820/150‑1 (MQ=255) ccAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCTGGTAGCTGCCGgcgc > 2:636329/1‑116 (MQ=255) ccAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCTGGTAGCTGCCGgcgc > 2:636324/1‑116 (MQ=255) ccAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCTGGTAGCTGCCGgcgc < 1:636329/116‑1 (MQ=255) ccAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCTGGTAGCTGCCGgcgc < 1:636324/116‑1 (MQ=255) ccAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGt > 1:112011/1‑150 (MQ=255) ccAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGt > 1:75041/1‑150 (MQ=255) ccAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGt < 1:161574/150‑1 (MQ=255) cAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTACACGAGAGTCGAACTTGCCGGCATCTACCAGCAGGAGAAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTc > 1:444710/1‑150 (MQ=255) gaccgaccTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTcgc < 1:464462/150‑1 (MQ=255) accTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTg > 2:218044/1‑150 (MQ=255) tGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGt > 1:722878/1‑150 (MQ=255) tGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGt < 2:722878/150‑1 (MQ=255) tGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGACAGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGt < 1:262288/150‑1 (MQ=255) aaGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGaa > 2:642544/1‑150 (MQ=255) tCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCTTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTCTTCCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCg < 2:151613/150‑1 (MQ=255) tCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCg < 1:642544/150‑1 (MQ=255) cAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCgg < 1:218044/150‑1 (MQ=255) aGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCggg < 2:112011/150‑1 (MQ=255) tCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTg < 2:444710/150‑1 (MQ=255) tCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGGACACCAGGAAGGTCGGGTAGGTg > 1:284254/1‑150 (MQ=255) tCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGt < 2:75041/150‑1 (MQ=255) gAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGc < 2:589205/150‑1 (MQ=255) ttCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAg < 2:97180/85‑1 (MQ=255) ttCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAg > 1:97180/1‑85 (MQ=255) ntCAGATAACCGGGTCCCAGCGTTGTGANCAGTCGCTGGCGCCGGCANCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCg > 1:109654/2‑150 (MQ=255) aGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAg > 1:190736/1‑128 (MQ=255) aGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAg < 2:190736/128‑1 (MQ=255) ccAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCa > 2:48717/1‑68 (MQ=255) ccAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCa < 1:48717/68‑1 (MQ=255) atCGCTGGCGCCGGCAGCTAACAGCCGGAGCAGGATCCCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATa < 1:393800/149‑1 (MQ=255) | GGTGGGGGTGATCACCCAGACCGACCTGATCGCCGGCCTCAAGCGTCAGTTGCTCAGCAAGATCGAGGCTTCAGATAACCGGGTCCCAGCGTTGTGACCAGTCGCTGGCGCCGGCAGCTACCAGCCGGCGCAGGATCGCGAAGGCCTGTTGCAGGGCTTCGCTGTCGCGTTTGCGTGGGTACACCAGGAAGGTCGGGTAGGTGAACTCCGGTGCCTGCGGTACCCGCTCGAACACGCCGCTGTCCAGATA > NC_002947/2680361‑2680610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |