Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,711,029 T→G 8.6% intergenic (+289/+25) PP_2373 → / ← PP_2374 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,711,0290TG 8.6% 146.1 / 5.8 58intergenic (+289/+25)PP_2373/PP_2374hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/30);  new base G (3/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTCGCGTGCGCGG  >  NC_002947/2710883‑2711177
                                                                                                                                                  |                                                                                                                                                    
ccGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCAc                                                                                                                                                   >  2:90732/1‑150 (MQ=255)
ccGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCAc                                                                                                                                                   <  1:108509/150‑1 (MQ=255)
ccGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCAc                                                                                                                                                   >  1:764580/1‑150 (MQ=255)
  gcgAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCAccc                                                                                                                                                 <  1:638748/150‑1 (MQ=255)
       aGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGaa                                                                                                                                            <  1:90732/150‑1 (MQ=255)
       aGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGaa                                                                                                                                            <  1:377886/150‑1 (MQ=255)
         ggccgggccTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACACCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAaga                                                                                                                                          >  2:260712/1‑150 (MQ=255)
           ccgggccTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAgg                                                                                                                                        >  2:832997/1‑150 (MQ=255)
              ggccTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCg                                                                                                                                     <  1:499743/150‑1 (MQ=255)
               gccTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCGACCGTATTCGCGGGTAAAGCCGCTCCTACGCCTGAAAGGGGGTGGGCACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCgg                                                                                                                                    >  1:505621/1‑150 (MQ=18)
                  tgctgGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGcac                                                                                                                                 >  2:589667/1‑150 (MQ=255)
                    ctgGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAg                                                                                                                               <  1:832997/150‑1 (MQ=255)
                       gCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGtt                                                                                                                            <  1:456039/150‑1 (MQ=255)
                         cAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAg                                                                                                                          >  2:178236/1‑150 (MQ=255)
                                cACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCa                                                                                                                   <  2:764580/150‑1 (MQ=255)
                                    tGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTg                                                                                                               >  1:505152/1‑150 (MQ=255)
                                       cGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAg                                                                                                            <  1:178236/150‑1 (MQ=255)
                                        gTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAgg                                                                                                           <  2:817947/150‑1 (MQ=255)
                                           cACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGcc                                                                                                        >  2:229508/1‑150 (MQ=255)
                                                   ccgccgCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCAc                                                                                                <  2:505152/150‑1 (MQ=255)
                                                       cgcTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGc                                                                                            <  1:229508/150‑1 (MQ=255)
                                                         cTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGccc                                                                                          <  2:850906/150‑1 (MQ=255)
                                                              gTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCa                                                                                     >  2:365101/1‑150 (MQ=255)
                                                                   cgcgGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTCAGAGGGTCAGGTGCAGGGCCCACTGCACCAGCCCGATCAATAcc                                                                                <  1:790270/150‑1 (MQ=255)
                                                                    gcgGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGc                                                                                                                         <  1:779263/108‑1 (MQ=255)
                                                                    gcgGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGc                                                                                                                         >  2:779263/1‑108 (MQ=255)
                                                                    gcgGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGAGAGGGTTCACCCGCGAGAGGGGGGGTACGAGTTATCGTACCAGGTGCCTGTCAGACTGCACCAGCCCGATCAATACCg                                                                               >  2:188089/1‑150 (MQ=17)
                                                                        gTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAAt                                                                           <  1:269169/150‑1 (MQ=255)
                                                                             cccGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTGAACCcgcgccta                                                                                                                                          <  1:816659/82‑5 (MQ=25)
                                                                             cccGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTGAACCcgcgccta                                                                                                                                          >  2:816659/1‑78 (MQ=37)
                                                                              ccGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAGCTGCACCa                                                                                              >  2:763772/1‑125 (MQ=255)
                                                                              ccGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCa                                                                                              <  1:763772/125‑1 (MQ=255)
                                                                                  tCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAAt                                                                                   >  1:439285/1‑132 (MQ=255)
                                                                                  tCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAAt                                                                                   <  2:439285/132‑1 (MQ=255)
                                                                                   ccTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAgg                                                                                                           >  2:483079/1‑107 (MQ=255)
                                                                                   ccTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAgg                                                                                                           <  1:483079/107‑1 (MQ=255)
                                                                                    cTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGt                                                               <  2:529571/150‑1 (MQ=255)
                                                                                     tACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGACAAGTCAAGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGTTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTg                                                              >  1:581832/1‑150 (MQ=255)
                                                                                             aaCGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGc                                                                                                                         <  2:306850/83‑1 (MQ=255)
                                                                                             aaCGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGc                                                                                                                         >  1:306850/1‑83 (MQ=255)
                                                                                             aaCGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAg                                                                                                            >  1:477548/1‑96 (MQ=255)
                                                                                             aaCGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAg                                                                                                            <  2:477548/96‑1 (MQ=255)
                                                                                                  gggTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAg                                                                                                            <  2:431881/91‑1 (MQ=255)
                                                                                                  gggTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAg                                                                                                            >  1:431881/1‑91 (MQ=255)
                                                                                                              gTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGt                                     <  2:505621/150‑1 (MQ=255)
                                                                                                                        ggACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCtt                           >  2:59086/1‑150 (MQ=255)
                                                                                                                        ggACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCtt                           <  1:188089/150‑1 (MQ=255)
                                                                                                                            acaGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAg                                      >  2:737796/1‑135 (MQ=255)
                                                                                                                            acaGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAg                                      <  1:737796/135‑1 (MQ=255)
                                                                                                                              aGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAACTGGCTGGCCTTGCCGTg                     <  1:589667/150‑1 (MQ=255)
                                                                                                                               ggCCGCTGTAGGTTCGGGGTTTCCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTgg                    <  2:581832/150‑1 (MQ=255)
                                                                                                                               ggCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTgg                    >  2:568986/1‑150 (MQ=255)
                                                                                                                                    cTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGaa               <  2:527207/150‑1 (MQ=255)
                                                                                                                                     tGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAg              >  1:325873/1‑150 (MQ=255)
                                                                                                                                       tAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTc            <  1:59086/150‑1 (MQ=255)
                                                                                                                                         ttcgcgGGTGAACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGa                                                                                        >  2:73474/3‑72 (MQ=25)
                                                                                                                                         ttcgcgGGTGAACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGa                                                                                        <  1:73474/70‑1 (MQ=25)
                                                                                                                                         ttcgcgGGTGAACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTcgc          >  2:380778/3‑150 (MQ=255)
                                                                                                                                                 ttCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTCGCGTGCGCgg  >  2:36506/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGCGAAAGGGCCGGGCCTGCTGGCCAATTTCCACCTGCCGTTCACCGTTGCCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACGCCTGAACGAGGGTGGACACAGGTCGGGGGTTGGACACAGGCCGCTGTAGGCGCGGGTTCACCCGCGAAGAGGCCGGCACAGGTTAGCGGGTCAGGTGCAGGGCCAACTGCACCAGCCCGATCAATACCGCAATGAACACCAGGGTGAACAGCGCCCCCAGCGCAATGAAGTGGCTGGCCTTGCCGTGGGTGAAGTCGCGTGCGCGG  >  NC_002947/2710883‑2711177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑