| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,713,709 | C→T | 11.1% | G480S (GGC→AGC) | metH ← | cobalamin‑dependent methionine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,713,709 | 0 | C | T | 11.1% | 90.7 / 3.5 | 36 | G480S (GGC→AGC) | metH | cobalamin‑dependent methionine synthase |
| Reads supporting (aligned to +/- strand): ref base C (16/16); new base T (2/2); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
AAGATGATGTCTTCTGGCGGGAAGCCCACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCGATCACTTCCCACTTGGACGAGTCGATCATGATCGGTACGCGC > NC_002947/2713576‑2713857 | aaGATGATGTCTTCTGGCGGGAAGCCCACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAgg > 1:485110/1‑150 (MQ=255) atgTCTTCTGGCGGGAAGCCCACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAggcgggcg > 1:379168/1‑150 (MQ=255) gTCTTCTGGCGGGAAGCCCACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCgtg < 1:354279/150‑1 (MQ=255) cttctGGCGGGAAGCCCACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCgtggt > 1:823471/1‑150 (MQ=255) cccACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCg > 2:633815/1‑150 (MQ=255) ccACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGa > 2:848389/1‑150 (MQ=255) ccACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGa < 2:485110/150‑1 (MQ=255) cACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGAc < 2:555854/150‑1 (MQ=255) cACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGAc > 1:795559/1‑150 (MQ=255) aGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGCCTTCGTATCACTCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATg < 1:574566/150‑1 (MQ=255) gATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATa < 2:758076/96‑1 (MQ=255) gATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATa > 1:758076/1‑96 (MQ=255) aTAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGACGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGa > 2:254637/1‑150 (MQ=255) aTAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGa < 1:184594/150‑1 (MQ=255) aTAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGa > 2:664430/1‑150 (MQ=255) gCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGtt < 2:386308/150‑1 (MQ=255) gcTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGa < 2:379168/150‑1 (MQ=255) gCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGAtgcc > 1:716405/1‑150 (MQ=255) gCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCGCGACGCCTTCCTTCATGGGAATGGAGTTGACGAtgcc > 2:327096/1‑150 (MQ=255) cAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGAtgccc < 2:823471/150‑1 (MQ=255) ccTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGAtgcccttgccct < 2:716405/150‑1 (MQ=255) gCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTACCCGGGATGCACTTGAGGACGGCTTca > 2:102744/1‑149 (MQ=255) ccGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCGATCACt < 1:633815/150‑1 (MQ=255) cGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCGATCACtt < 1:664430/150‑1 (MQ=255) gACTTCGTCGAAGGCCATGTGCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGa < 1:224334/131‑1 (MQ=255) gACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCACTGGATGCACTTGa > 2:224334/1‑131 (MQ=255) ttCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGAc < 2:377473/75‑1 (MQ=255) ttCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGAc > 1:377473/1‑75 (MQ=255) aaGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCGATCACTTCCCACTTGGAc > 2:312147/1‑150 (MQ=255) ccATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCGATCACTTCCCACTTGGACGAGt < 1:848389/150‑1 (MQ=255) tacggatgcgcCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCGATCACTTCCCACTTGGACGAGTCGATCATGAt < 1:102744/143‑1 (MQ=255) acGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCGATCACTTCCCACTTGGACGAGTCGATCATGATc > 1:435314/1‑150 (MQ=255) caaGCGCTATGGCGC‑TGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCg < 1:395340/111‑1 (MQ=255) caaGCGCTATGGCGC‑TGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCg > 2:395340/3‑113 (MQ=255) aGCGCTATGGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGAt < 2:745875/78‑1 (MQ=255) aGCGCTATGGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGAt > 1:745875/1‑78 (MQ=255) ccATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCGATCACTTCCCACTTGGACGAGTCGATCATGATCGGTAcgcgc > 1:455743/1‑150 (MQ=255) | AAGATGATGTCTTCTGGCGGGAAGCCCACTTCGTTGACCAGGATGTCATAGCTGCGCTGGCAGATTTCCTTCTTGCGCGCGGCTGTGTCGGCCTGGCCGACTTCGTCGAAGGCCATGACCACCACGGCAGCGCCATAGCGCTTGCACAGGCGGGCGTGGTGCTTGAACTGCTCGACGCCTTCCTTCATGGAAATGGAGTTGACGATGCCCTTGCCCTGGATGCACTTGAGGCCGGCTTCGATCACTTCCCACTTGGACGAGTCGATCATGATCGGTACGCGC > NC_002947/2713576‑2713857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |