Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,731,871 G→T 14.2% A389S (GCG→TCG)  polB → DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,731,8710GT14.2% 75.1 / 3.5 28A389S (GCG→TCG) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (12/12);  new base T (2/2);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CACGCGCATTTTCGCCCATACCCGGCTGCTCGACTTCCTGCTCGAACGTGCCTCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGAGCCTGTACCCGTCGATCATCCGGACCTTTCTCATCGACCCGGTCGGG  >  NC_002947/2731726‑2732020
                                                                                                                                                 |                                                                                                                                                     
cACGCGCATTTTCGCCCATACCCGGCTGCTCGACTTCCTGCTCGAACGTGCCTCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGcc                                                                                                                                                   <  2:257888/150‑1 (MQ=255)
     gcATTTTCGCCCATACCCGGCTGCTCGACTTCCTGCTCGAACGTGCCTCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGcc                                                                                                                                              <  1:328240/150‑1 (MQ=255)
                     ccGGCTGCTCGACTTCCTGCTCGAACGTGCCTCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGa                                                                                                                              >  1:301848/1‑150 (MQ=255)
                                  ttCCTGCTCGAACGTGCCTCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGcccc                                                                                                                 <  2:729752/150‑1 (MQ=255)
                                       gCTCGAACGTGCCTCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCcggcgg                                                                                                            >  1:366395/1‑150 (MQ=255)
                                        cTCGAACGTGCCTCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCggg                                                                                                           >  2:305477/1‑150 (MQ=255)
                                           gAACGTGCCTCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGttt                                                                                                        <  2:341094/150‑1 (MQ=255)
                                                    tCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGAt                                                                                               >  2:251828/1‑150 (MQ=255)
                                                       gTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTcg                                                                                            >  1:699149/1‑150 (MQ=255)
                                                         gACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTcgcg                                                                                          <  2:301848/150‑1 (MQ=255)
                                                                   gCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGc                                                                                                                                         <  2:766322/93‑1 (MQ=255)
                                                                   gCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGc                                                                                                                                         >  1:766322/1‑93 (MQ=255)
                                                                   gCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCtt                                                                                <  2:251418/150‑1 (MQ=255)
                                                                                      ggTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCggtgctggtgc                                                             <  1:681656/150‑1 (MQ=255)
                                                                                          ggTAGCCGCGTTCTGTCACCTGTACATGCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGa                                                         <  2:274156/150‑1 (MQ=255)
                                                                                                                    ttCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGAGCCTGTACCCGTCGatca                               <  2:699149/150‑1 (MQ=255)
                                                                                                                       cacaGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGAGCCTGTACCCGTCGATCATcc                            >  2:15350/1‑150 (MQ=255)
                                                                                                                        acaGATGCACCGCCTTGGCTTTGTCTCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCg                                                                       <  1:624/106‑1 (MQ=255)
                                                                                                                        acaGATGCACCGCCTTGGCTTTGTCTCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCg                                                                       <  1:567/106‑1 (MQ=255)
                                                                                                                        acaGATGCACCGCCTTGGCTTTGTCTCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCg                                                                       >  2:624/1‑106 (MQ=255)
                                                                                                                        acaGATGCACCGCCTTGGCTTTGTCTCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCg                                                                       >  2:567/1‑106 (MQ=255)
                                                                                                                        acaGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGGCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGAGCCTGTACCCGTCGATCATCCg                           >  2:154961/1‑150 (MQ=255)
                                                                                                                                  cGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGAGCCTGTACCCGTCGATCATCCGGACCTTtctc                 >  1:528960/1‑150 (MQ=255)
                                                                                                                                   gCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTc                                                                        <  1:559628/94‑1 (MQ=255)
                                                                                                                                   gCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTc                                                                        >  2:559628/1‑94 (MQ=255)
                                                                                                                                    ccTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGAGCCTGTACCCGTCGATCATCCGGACCTTTCTCAt               >  1:549544/1‑150 (MQ=255)
                                                                                                                                        ggCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGgtgctggtgctg                                                           >  2:617214/1‑102 (MQ=255)
                                                                                                                                        ggCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGgtgctggtgctg                                                           <  1:617214/102‑1 (MQ=255)
                                                                                                                                                 gcgcCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCTGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGATCCTGTACCCGTCGATCATCCGGACCTTTCTCATCGACCCGGTCggg  >  1:18860/1‑150 (MQ=255)
                                                                                                                                                 gcgcCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGAGCCTGTACCCGTCGATCATCCGGACCTTTCTCATCGACCCGGTCggg  <  2:366395/150‑1 (MQ=255)
                                                                                                                                                 gcgcCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGAGCCTGTACCCGTCGATCATCCGGACCTTTCTCATCGACCCGGTCggg  >  2:230032/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CACGCGCATTTTCGCCCATACCCGGCTGCTCGACTTCCTGCTCGAACGTGCCTCGGTGACGGGATTGGCCGTGGACCGCAGCGGTGGGTCGGTAGCCGCGTTCTGTCACCTGTACATTCCACAGATGCACCGCCTTGGCTTTGTCGCGCCCAGCCTTGGCAGCCGGCCGGACGAAGCCAGCCCCGGCGGGTTTGTCATGGATTCGCGGCCAGGGCTTTACGACTCGGTGCTGGTGCTGGACTACAAGAGCCTGTACCCGTCGATCATCCGGACCTTTCTCATCGACCCGGTCGGG  >  NC_002947/2731726‑2732020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑