Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,802,435 T→C 17.9% L259P (CTG→CCG) ‡ rbsA‑I → ribose ABC transporter ‑ ATP‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,802,4350TC17.9% 52.7 / 5.6 28L259P (CTG→CCG) ‡rbsA‑Iribose ABC transporter ‑ ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (9/14);  new base C (2/3);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

GCATCGCCTGGAAGAGTTGCAGCGCGTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGC  >  NC_002947/2802286‑2802583
                                                                                                                                                        |                                                                                                                                                    
gCATCGCCTGGAAGAGTTGCAGCGCGTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCt                                                                                                                                                      >  1:581201/1‑150 (MQ=255)
gCATCGCCTGCAAGAGTTGCATCGCGTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCt                                                                                                                                                      >  2:35995/1‑150 (MQ=255)
  aTCGCCTGGAAGAGTTGCAGCGCGTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTgg                                                                                                                                                    <  1:759775/150‑1 (MQ=255)
          gAAGAGTTGCAGCGCGTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCCAGGTCGatat                                                                                                                                            <  1:451819/150‑4 (MQ=255)
            agagTTGCAGCGCGTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCa                                                                                                                                          <  2:581201/150‑1 (MQ=255)
               gTTGCAGCGCGTGGCCCAACGCACCGTTGTGGCGCGCAATCAGACCAGC‑GTTG‑GCGCTTAGCGCATCCGGCTCTACAGCAGCGCCGAGCTGGTCAACCTGACGGTCGGCCGCGAGCTAAG‑CGAGATTTTCTACCTGGTTTGTCGGCAGtt                                                                                                                                       <  2:401822/150‑1 (MQ=255)
                ttGCAGCGCGTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTg                                                                                                                                      >  2:413681/1‑150 (MQ=255)
                 tGCAGCGCGTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGTCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTgg                                                                                                                                     <  1:50769/150‑1 (MQ=255)
                         gTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCg                                                                                                                             >  1:163371/1‑150 (MQ=255)
                         gTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCCAGGTCGatat                                                                                                                                            <  1:199452/135‑4 (MQ=255)
                         gTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCCAGGTCGatat                                                                                                                                            >  2:199452/1‑132 (MQ=255)
                                       cGTTGGGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGGCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGa                                                                                                               <  2:597155/150‑1 (MQ=255)
                                           gtgCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACaaa                                                                                                           <  1:95106/150‑1 (MQ=255)
                                                           cAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGa                                                                                             <  1:413681/150‑1 (MQ=255)
                                                            aaGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCCAGGTCGatat                                                                                                                                            <  1:463003/102‑4 (MQ=255)
                                                            aaGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCCAGGTCGatat                                                                                                                                            >  2:463003/1‑99 (MQ=255)
                                                               cTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAg                                                                                         <  1:35995/150‑1 (MQ=255)
                                                               cTGGTGTGCGACAAGCCGAGCTAGGTCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCG‑GCGCTGGGCCGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAg                                                                                         <  1:270596/150‑1 (MQ=255)
                                                                          cgaGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGgt                                                                              <  2:163371/150‑1 (MQ=255)
                                                                              ccGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCg                                                                          <  1:29367/150‑1 (MQ=255)
                                                                                       cgcTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTc                                                                 <  1:465535/150‑1 (MQ=255)
                                                                                          tACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGt                                                                                                  <  2:136505/114‑1 (MQ=255)
                                                                                          tACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGt                                                                                                  >  1:136505/1‑114 (MQ=255)
                                                                                          aacggcagcGCCGAGCTGGTCAACATGATGGTCGGCCGCGAGCTGGC‑CGAACAAAACGACCTGGGTCGACGGCAGTTGGGTGCACAGCTGCTCAAGGTCGACAAACTGTGCCGTGTCGACAAGGTGCGCGAGGGGTCGTTCGAAGTcgga                                                              >  2:300260/5‑147 (MQ=255)
                                                                                               cagcGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTcagggcagg                                                         <  1:242746/150‑1 (MQ=255)
                                                                                                        gCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGAtttt                                                >  1:778356/1‑150 (MQ=255)
                                                                                                                aCCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCAtct                                        >  2:748346/1‑150 (MQ=255)
                                                                                                                      tGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGcc                                  >  1:222229/1‑150 (MQ=255)
                                                                                                                                                       cTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTgcgc  >  1:653242/1‑150 (MQ=255)
                                                                                                                                                       cTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTgcgc  >  1:439259/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                    
GCATCGCCTGGAAGAGTTGCAGCGCGTGGCCCAACGCATCGTTGTGCTGCGCGAT‑‑GGCAAGCTGGTGTGCGACGAGCCGATCCAGCGCTACAGCAGCGCCGAGCTGGTCAACCTGATGGTCGGCCGCGAGCTGGG‑CGAGCATATCGACCTGGGTCGACGGCAGTTGGGTGCACCGCTGCTCAAGGTCGACAAACTGTGCCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGC  >  NC_002947/2802286‑2802583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑