| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,852,440 | T→A | 11.8% | Q312L (CAG→CTG) | PP_2505 ← | GAF domain/GGDEF domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,852,440 | 0 | T | A | 11.8% | 93.5 / 4.4 | 34 | Q312L (CAG→CTG) | PP_2505 | GAF domain/GGDEF domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (15/15); new base A (2/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
AATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAG > NC_002947/2852295‑2852573 | aaTGTGATGCGTACCCTCCCGGAGCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGAAAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGt < 2:286304/150‑1 (MQ=255) aTGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTg < 1:224746/150‑1 (MQ=255) gtgATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAg < 1:10949/150‑1 (MQ=255) aTGCGTACCCTCCCGGATCCGGAGCTCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAg > 1:467933/1‑150 (MQ=255) aTGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAg < 1:609818/150‑1 (MQ=255) cGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTCCTCTGCCTGGGTGAGCAGGGCGCACCCAATg > 1:217491/1‑150 (MQ=255) tCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAACCTGCTCACCCAGGGTGAGCAgattgat > 1:231145/1‑133 (MQ=255) tCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAACCTGCTCACCCAGGGTGAGCAgattgat < 2:231145/139‑7 (MQ=255) cTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAggg > 1:622731/1‑126 (MQ=255) cTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAggg < 2:622731/126‑1 (MQ=255) ttAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCAt > 2:292358/1‑141 (MQ=255) ttAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCAt < 1:292358/141‑1 (MQ=255) gATAACACTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGccgccg < 2:155713/150‑1 (MQ=255) gACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGgcgc > 1:121661/1‑150 (MQ=255) ttGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATg < 2:669284/106‑1 (MQ=255) ttGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATg > 1:669284/1‑106 (MQ=255) tCACGTCCATCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATCGTCATCGTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGAtt < 2:110804/150‑1 (MQ=255) tCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGAtt < 2:121661/150‑1 (MQ=255) gTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGAtt < 1:306061/146‑1 (MQ=255) gTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGAtt > 2:306061/1‑146 (MQ=255) gTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATtgacc > 2:133432/1‑149 (MQ=255) caccaTGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATtgactgac < 2:467933/150‑1 (MQ=255) caTGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCa > 2:312761/1‑150 (MQ=255) caTGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCa > 2:490510/1‑150 (MQ=255) aCCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgcgc > 1:679698/1‑150 (MQ=255) gcgcgcTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTgg < 1:312761/150‑1 (MQ=255) ttGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGGACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTaa > 2:495515/1‑150 (MQ=255) atcatcAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGgcc < 1:543621/66‑1 (MQ=255) atcatcAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGgcc > 2:543621/1‑66 (MQ=255) atcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCTGACGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAg > 1:416043/1‑150 (MQ=255) atcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCaa > 1:718197/1‑105 (MQ=255) atcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCaa < 2:718197/105‑1 (MQ=255) cTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTAt > 1:403737/1‑131 (MQ=255) cTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTAt < 2:403737/131‑1 (MQ=255) | AATGTGATGCGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAG > NC_002947/2852295‑2852573 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |