| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,871,819 | C→T | 11.8% | S185L (TCG→TTG) | PP_2524 → | HD domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,871,819 | 0 | C | T | 11.8% | 83.2 / 3.9 | 34 | S185L (TCG→TTG) | PP_2524 | HD domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (15/15); new base T (2/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
ATTGCCCGGTGTTTTTACGTCGCCGGGCGAATGGGGTCGGCGCTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCGGGGCAGGTTAGTCATGCCGGGCTTGTACCGTCGTGCTTGAGC > NC_002947/2871680‑2871953 | aTTGCCCGGTGTTTTTACGTCGCCGGGCGAATGGGGTCGGCGCTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTaaa > 1:42741/1‑150 (MQ=255) ttGCCCGGTGTTTTTACGTCGCCGGGCGAATGGGGTCGGCGCTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTaaaa > 2:610556/1‑150 (MQ=255) cGGTGTTTTTACGTCGCCGGGCGAATGGGGTCGGCGCTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCaa > 1:455906/1‑150 (MQ=255) ggTGTTTTTACGTCGCCGGGCGAATGGGGTCGGCGCTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACGAGGACCGGAACTATTGCAGTGGGCGCTTCTAGACCAAACTGCTCAAGCTCGCCTTGGGCTTTAAAACCCaag > 1:317034/1‑150 (MQ=255) gTCGCCGGGCGAATGGGGTCGGCGCTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGgcgcgc > 2:420032/1‑150 (MQ=255) aaTGGGGTCGGCGCTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGa < 2:455906/150‑1 (MQ=255) ggCGCTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATAc > 1:187613/1‑150 (MQ=255) gcTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTTGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCg > 1:219990/1‑150 (MQ=255) aTGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAggg > 2:115469/1‑116 (MQ=255) aTGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAggg < 1:115469/116‑1 (MQ=255) aCTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGgc > 1:367176/1‑115 (MQ=255) aCTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGgc < 2:367176/115‑1 (MQ=255) aCTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGaaa < 1:610556/150‑1 (MQ=255) aCTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGaaa < 1:420032/150‑1 (MQ=255) tCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTTGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCCGCTTGAAACCt < 2:317034/150‑1 (MQ=255) cGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCtt < 2:257380/150‑1 (MQ=255) gaATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCt < 2:780227/150‑1 (MQ=255) tCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGCCCCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCttcc < 2:760965/150‑3 (MQ=255) ccTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCCAACTGCTCAAGCTCGCCTCGGGCTTTaaaa < 1:23142/91‑1 (MQ=255) ccTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTaaaa > 2:23142/1‑91 (MQ=255) tttCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCa > 2:124704/1‑150 (MQ=255) gggCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACgaga < 1:124704/150‑1 (MQ=255) ccAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCgg < 2:42741/150‑1 (MQ=255) aGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCGGGGCAGGTTAGTCATGCCGGGCtt < 1:411927/150‑1 (MQ=255) ttCCAGACCAAACTGCTCAAGCTCGCCTTGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCGGGGCAGGTTAGTCATGCCGGGCTTGTAcc < 2:219990/150‑1 (MQ=255) gACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGAc < 2:36857/106‑1 (MQ=255) gACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGAc > 1:36857/1‑106 (MQ=255) gACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCGGGGCAGGTTAGTCATGCCGGGCTTGTACcgtcgt > 1:376452/1‑150 (MQ=255) cAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCGGGGCAGGTTAGTCATGCCGGGCTTGTACCGTCGTGCt > 1:76169/1‑150 (MQ=255) tGCTCAAGCTCGCCTCGGGCGTTAAAACTCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCGGGGCAGGTTAGTCATGCCGGGCTTGGACCGTCGTGCTTGAGc > 1:285950/1‑150 (MQ=255) gCTCAAGCTAGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCGGGGCAGGTTAGTCATGCCGGGCTTg > 2:36316/1‑132 (MQ=255) gCTCAAGCTAGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCGGGGCAGGTTAGTCATGCCGGGCTTg < 1:36316/132‑1 (MQ=255) tCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACga < 2:839257/91‑1 (MQ=255) tCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACga > 1:839257/1‑91 (MQ=255) | ATTGCCCGGTGTTTTTACGTCGCCGGGCGAATGGGGTCGGCGCTCTATGACTTCGAGAATCCTTTCGCGCAGCAGAGGGCGTACCAGGACGGGGCGTATGCCATCGAGCACTTCCAGACCAAACTGCTCAAGCTCGCCTCGGGCTTTAAAACCCAAGAAGGGGCGCGCCTTGCTGCCGAGCGTCATACCCGGCTTGAAACCTTTCTTACCCAGTTCATGGACGAGATTACCGGGGCAGGTTAGTCATGCCGGGCTTGTACCGTCGTGCTTGAGC > NC_002947/2871680‑2871953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |