Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,909,328 G→A 8.1% Q385* (CAG→TAG) ‡ aprDA ← alkaline protease secretion ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,909,3280GA 8.1% 136.6 / 2.9 49Q385* (CAG→TAG) ‡aprDAalkaline protease secretion ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

CTGTCCACCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGTGCTGCTGCCTGGGGCGCCGGCGAT  >  NC_002947/2909180‑2909472
                                                                                                                                                    |                                                                                                                                                
cTGTCAACCGCGGAAAAGCGGGCGATGTTTTCGGCGATGGTGCCTGCCAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTgg                                                                                                                                                 <  2:25156/150‑1 (MQ=255)
 tGTCCACCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTggg                                                                                                                                                >  2:127379/1‑150 (MQ=255)
     cACCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCCAggccgg                                                                                                                                            >  1:204017/1‑150 (MQ=255)
      aCCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGACCCGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGggccggc                                                                                                                                           <  2:672614/150‑1 (MQ=255)
        cTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCca                                                                                                                                         >  2:852872/1‑150 (MQ=255)
         tCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCCAggccggcaag                                                                                                                                        <  2:204017/150‑4 (MQ=255)
                     gCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAAc                                                                                                                            <  2:372726/150‑1 (MQ=255)
                       gATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTAc                                                                                                                                >  1:343459/1‑144 (MQ=255)
                       gATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTAc                                                                                                                                <  2:343459/144‑1 (MQ=255)
                           ttttCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAggcgg                                                                                                                      >  2:851479/1‑150 (MQ=255)
                            tttCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAAc                                                                                                                            <  2:626455/143‑1 (MQ=255)
                            tttCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAAc                                                                                                                            >  1:626455/1‑143 (MQ=255)
                                 gCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGTACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAgg                                                                                                                <  2:223172/150‑1 (MQ=255)
                                        tGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAAc                                                                                                                            >  1:314412/1‑131 (MQ=255)
                                        tGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAAc                                                                                                                            <  2:314412/131‑1 (MQ=255)
                                                      tCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCCGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAAc                                                                                                                            >  2:634316/1‑117 (MQ=255)
                                                      tCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAAc                                                                                                                            <  1:634316/117‑1 (MQ=255)
                                                      tCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCccgaggccgag                                                                                           >  1:308527/1‑150 (MQ=255)
                                                        cACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAggg                                                                                         <  2:15778/150‑1 (MQ=255)
                                                             ccTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGa                                                                                    >  2:710550/1‑150 (MQ=255)
                                                                   gCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGacac                                                                              >  2:255904/1‑150 (MQ=255)
                                                                        tAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCggg                                                                         <  1:851479/150‑1 (MQ=255)
                                                                            ccAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAg                                                                                                                          <  2:176151/97‑1 (MQ=255)
                                                                            ccAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAg                                                                                                                          >  1:176151/1‑97 (MQ=255)
                                                                            ccAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAAGCCGAGGGGCCGATGACACCCAGGCTGt                                                                     >  2:90215/1‑150 (MQ=255)
                                                                                     ggCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCgggg                                                            <  1:255904/150‑1 (MQ=255)
                                                                                      gCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGc                                                           <  2:278825/150‑1 (MQ=255)
                                                                                       cccAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGcc                                                          >  1:209284/1‑150 (MQ=255)
                                                                                           gTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGc                                                      <  1:68297/150‑1 (MQ=255)
                                                                                                  cTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCt                                               >  1:553120/1‑150 (MQ=255)
                                                                                                  cTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCt                                               >  1:353432/1‑150 (MQ=255)
                                                                                                      ttCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCAcc                                           >  1:274236/1‑150 (MQ=255)
                                                                                                      ttCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCAcc                                           >  2:595891/1‑150 (MQ=255)
                                                                                                      ttCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCAcc                                           >  2:361017/1‑150 (MQ=255)
                                                                                                        ccAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCAcccc                                         <  2:274236/150‑1 (MQ=255)
                                                                                                          aGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCcgc                                       <  2:553120/150‑1 (MQ=255)
                                                                                                           ggTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCcgcg                                      >  2:805063/1‑150 (MQ=255)
                                                                                                            gTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGt                                     >  1:468512/1‑150 (MQ=255)
                                                                                                              gAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTAc                                   <  2:353171/150‑1 (MQ=255)
                                                                                                                  atatCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGGAGACTTGCCCGAGGCCGGGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCCGGCTGAAGCTCACCCCGCGTACGAtc                               >  1:313871/1‑149 (MQ=255)
                                                                                                                     tCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTg                            <  1:361017/150‑1 (MQ=255)
                                                                                                                       ggCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGt                          >  1:214916/1‑150 (MQ=255)
                                                                                                                        gcgcCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAgg                                                                         <  1:924/102‑1 (MQ=255)
                                                                                                                        gcgcCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAgg                                                                         >  2:924/1‑102 (MQ=255)
                                                                                                                            cGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGtgctgc                     <  2:308527/150‑1 (MQ=255)
                                                                                                                             gTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGtgctgct                    <  2:209284/150‑1 (MQ=255)
                                                                                                                                  ggCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGTGCTGCTGCCTg               <  1:382339/150‑1 (MQ=255)
                                                                                                                                   gcgcACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGTGCTGCTGCCTgg              <  2:468512/150‑1 (MQ=255)
                                                                                                                                            gCCGGCCCAGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCccgaggccgagg                                                                                          <  2:387119/65‑1 (MQ=255)
                                                                                                                                            gCCGGCCCAGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCccgaggccgagg                                                                                          >  1:387119/1‑65 (MQ=255)
                                                                                                                                               ggCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGTGCTGCTGCCTGGGGCGCCGGCGAt  <  1:595891/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CTGTCCACCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACCTTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGTGCTGCTGCCTGGGGCGCCGGCGAT  >  NC_002947/2909180‑2909472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑