Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 202,062 T→A 20.8% L2523Q (CTG→CAG)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947202,0620TA20.8% 78.7 / 14.5 38L2523Q (CTG→CAG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (16/14);  new base A (3/5);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTA  >  NC_002947/201913‑202204
                                                                                                                                                     |                                                                                                                                              
ggTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCt                                                                                                                                                <  2:762516/150‑1 (MQ=255)
ggTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCt                                                                                                                                                <  1:507336/150‑1 (MQ=255)
        cTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGTGa                                                                                                                                        <  2:486408/150‑1 (MQ=255)
              tCACTGACGAGCCAGTCACTCCAGGCAACGAAGGCGACATCGTCAAGATCACCATCACGGCCGACCAGACTTCGGTAGCCGATAACGTCAAGCCACCCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGTGagccagc                                                                                                                                  <  2:741940/150‑7 (MQ=14)
                        gCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGTGagccagc                                                                                                                                  <  2:272241/140‑7 (MQ=255)
                        gCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGTGAgccagc                                                                                                                                  >  1:272241/1‑137 (MQ=255)
                                   cAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCACCCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTCTTCGTCACCCTGAGCAACAACGCTCAGGTccccc                                                                                                             >  2:311894/1‑149 (MQ=14)
                                    aGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACCACGCTCAGGTCAccct                                                                                                            >  2:172652/1‑148 (MQ=255)
                                    aGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGTGAgcca                                                                                                                                    >  1:152636/1‑125 (MQ=255)
                                    aGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGTGAgcca                                                                                                                                    <  2:152636/126‑2 (MQ=255)
                                               gCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTg                                                                                                 >  2:590615/1‑150 (MQ=255)
                                                cGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTga                                                                                                <  1:796822/150‑1 (MQ=255)
                                                 gACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTgag                                                                                               >  1:746738/1‑150 (MQ=255)
                                                    aTCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGAcc                                                                                            >  1:740870/1‑150 (MQ=255)
                                                     gcGTCAAGGTCACCATCACGGCCGACAAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCa                                                                                           <  2:280492/149‑1 (MQ=255)
                                                       gTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCaa                                                                                                         >  2:88732/1‑134 (MQ=40)
                                                       gTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCaa                                                                                                         <  1:88732/134‑1 (MQ=255)
                                                       gTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGt                                                                                         >  2:198688/1‑150 (MQ=255)
                                                                aCCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTac                                                                                >  1:296280/1‑150 (MQ=255)
                                                                 ccATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTaca                                                                               <  1:719242/150‑1 (MQ=255)
                                                                               cAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCaa                                                                 <  1:845796/150‑1 (MQ=255)
                                                                                  aCTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGc                                                              <  2:628739/150‑1 (MQ=255)
                                                                                       ggTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGa                                                         >  2:712054/1‑150 (MQ=255)
                                                                                                    gTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGACCGGTGAGACCAGTGCGCCGTACACCCACGACACGCAAGGCGATGACGTCTACCAGGAc                                            >  2:245178/1‑150 (MQ=255)
                                                                                                          ccAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGc                                      <  1:198688/150‑1 (MQ=255)
                                                                                                          ccAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGc                                      <  2:740870/150‑1 (MQ=255)
                                                                                                            taCCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCa                                    >  2:124528/2‑150 (MQ=255)
                                                                                                               cTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTg                                        <  1:686865/143‑1 (MQ=255)
                                                                                                               cTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTg                                        >  2:686865/1‑143 (MQ=255)
                                                                                                                    ccGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGcc                            >  1:332236/1‑150 (MQ=255)
                                                                                                                      gTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCCGCCTg                          <  1:124528/150‑1 (MQ=255)
                                                                                                                      gTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTg                          <  1:712054/150‑1 (MQ=255)
                                                                                                                                 ccAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTc               >  1:841703/1‑150 (MQ=255)
                                                                                                                                 ccAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTc               >  2:738291/1‑150 (MQ=255)
                                                                                                                                       tcagggtCACGACCAGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCtgt         >  1:778116/7‑150 (MQ=255)
                                                                                                                                       gCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCtgt         <  1:311894/150‑1 (MQ=255)
                                                                                                                                          ggCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGa      <  2:811968/150‑1 (MQ=255)
                                                                                                                                              cACGACCAGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTa  <  2:778116/150‑1 (MQ=255)
                                                                                                                                               aCGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTcc              >  2:71441/1‑137 (MQ=255)
                                                                                                                                               aCGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTcc              <  1:71441/137‑1 (MQ=255)
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GGTCAGCACTACCGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTA  >  NC_002947/201913‑202204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑